[1]刘兆明,钟小仙,钱晨,等.苏丹草管状分子体外诱导及木质素合成相关酶活力的变化[J].江苏农业学报,2015,(04):846-854.[doi:10.3969/j.issn.1000-4440.2015.04.022]
　LIU Zhao-ming,ZHONG Xiao-xian,QIAN Chen,et al.In vitro induction of tracheary elements of Sorghum sudanense and changes in lignin synthesis related enzymes activities[J].,2015,(04):846-854.[doi:10.3969/j.issn.1000-4440.2015.04.022]
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苏丹草管状分子体外诱导及木质素合成相关酶活力的变化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
846-854
栏目:
畜牧兽医·水产养殖
出版日期:
2015-08-31

文章信息/Info

Title:
In vitro induction of tracheary elements of Sorghum sudanense and changes in lignin synthesis related enzymes activities
作者:
刘兆明12; 钟小仙2; 钱晨2; 刘大林1
(1.扬州大学动物科学与技术学院，江苏扬州225009；2.江苏省农业科学院畜牧研究所，江苏南京210014)
Author(s):
LIU Zhao-ming12; ZHONG Xiao-xian2; QIAN Chen2; LIU Da-lin1
(1.College of Animal Science and Technology，Yangzhou University，Yangzhou 225009，China；2.Institute of Animal Science，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China) 
关键词:
苏丹草; 管状分子; 体外诱导; 木质素合成
Keywords:
sudangrass(Sorghum sudanense); tracheary element; in vitro induction; lignin synthesis
分类号:
S544+.1
DOI:
10.3969/j.issn.1000-4440.2015.04.022
文献标志码:
A
摘要:
为探索C4作物次生细胞壁合成机制，以苏丹草新品系苏牧3号 的胚性愈伤组织为材料，研究了光照、外源植物激素和活性炭对愈伤组织管状分子分化的影响，并对管状分子诱导过程中木质素含量、可溶性酚醛组分含量及其相关酶活力变化进行了分析。结果表明，在每天光照16 h、不含外源植物激素的基本培养基中添加3 g/L活性炭，诱导培养20 d，管状分子分化率最高，为51.97%，管状分子出现的起始时间为诱导培养第4 d；诱导培养21 d时，木质素含量达到最高值 18.11%，与木质素合成相关的酶PAL、CAD活力也同时达到最高值，分别为 5.915 0×10-3〖KG*3〗U/mg、8.366 7×10-2〖KG*3〗U/mg，此后木质素含量和PAL、CAD酶活力均无显著变化；POD活力诱导培养9 d时达到最高值 132.24 U/mg，显著高于继代培养；可溶性酚醛组分含量先增后减，诱导培养12 d时达到最高值 1.09 μg/mg。
Abstract:
To reveal the secondary cell wall synthesis mechanism of C4 crops, the effect of illumination, exogenous hormone and activated carbon concentration on tracheary elements induction in vitro was studied on sudangrass Sumu No.3. The changes of lignin content, soluble phenolic components contents and related enzymes activities were analyzed in the process of tracheary elements induction as well. The results showed that the differentiation rate of tracheary elements reached the highest point (51.97%) at 20th day induction in 16-h daily illumination when calli were cultured in plant hormone-free MS medium with 3 g/L activated carbon addition. After 21-d induction, ligin content get to the peak value of 18.11%, and so did the activities of enzymes PAL (phenylalanine ammonia lyase and CAD (cinnamyl alcohol dehydrogenase, which were shown to be 5.915 0×10-3 U/mg and 8.366 7×10-2〖KG*3〗U/mg， respectively. The lignin content and enzymatic activities did not changed significantly afterwards. The activity of enzyme POD (peroxidase) came to its maximum value of 132.24 U/mg after 12-d induction. Soluble phenolic components content increased over time and reached the highest of 1.09 μg/mg after 12-d induction 12th day, and then decreased.

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备注/Memo

备注/Memo:
收稿日期：2015-03-26 基金项目：国家牧草产业技术体系综合试验站项目(CARS-35)；江苏省农业科技自主创新基金项目[CX(13)3035];江苏省第四期“333工程”培养资金资助项目 作者简介：刘兆明(1990-)，男，山东潍坊人，硕士研究生，主要从事牧草育种研究。(Email)lzzm2012@163.com 通讯作者：钟小仙，(Tel)025-84390239 ；(E-mail)zhpansy@aliyun.com
更新日期/Last Update: 2015-08-31