[1]杨丹,姚金保,杨学明,等.北方麦区小麦品种高分子量谷蛋白亚基组成及其与品质性状的关系[J].江苏农业学报,2015,(02):241-246.[doi:10.3969/j.issn.1000-4440.2015.02.003]
 YANG Dan,YAO Jin-bao,YANG Xue-ming,et al.High molecular weight gluten subunit(HMW-GS) composition of wheat cultivars in northern region and its relationship with quality traits[J].,2015,(02):241-246.[doi:10.3969/j.issn.1000-4440.2015.02.003]
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北方麦区小麦品种高分子量谷蛋白亚基组成及其与品质性状的关系()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
241-246
栏目:
遗传育种·生理生化
出版日期:
2015-04-30

文章信息/Info

Title:
High molecular weight gluten subunit(HMW-GS) composition of wheat cultivars in northern region and its relationship with quality traits
作者:
杨丹1姚金保1杨学明1周淼平1马鸿翔1王亚松2
(1.江苏省农业科学院 江苏省农业生物学重点实验室,江苏南京210014;2.江苏红旗种业股份有限公司,江苏泰州225300)
Author(s):
YANG Dan1YAO Jin-bao1YANG Xue-ming1ZHOU Miao-ping1MA Hong-xiang1WANG Ya-song2
(1.Provincial Key Laboratory of Agrobiology / Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Jiansu Hongqi Seed Industry Co.〖KG-*4〗, Ltd.〖KG-*4〗, Taizhou 225300, China)
关键词:
小麦高分子量谷蛋白亚基蛋白质含量沉降值
Keywords:
wheat varietyhigh molecular weight glutenin subunit(HMW-GS)protein contentsedimentation value
分类号:
S634.3
DOI:
10.3969/j.issn.1000-4440.2015.02.003
文献标志码:
A
摘要:
为了解北方麦区小麦品种的遗传多样性,挖掘可供利用的优异高分子量谷蛋白亚基(HMW-GS)类型,为品质改良提供基础材料,利用聚丙烯酰胺凝胶电泳方法(SDS-PAGE),分析了172份北方麦区部分小麦品种的HMW-GS组成,并对其与蛋白质含量和沉降值之间的关系进行了研究。结果表明:Glu-A1、Glu-B1和Glu-D1分别具有3种、6种和3种不同的亚基类型。亚基1、7+9和5+10在各自位点上出现的频率最高,分别达到了54.07%、48.26%和49.42%。3个位点亚基对蛋白质含量的效应可分别表示为:1≥2*>Null,13+16≥14+15≥17+18≥7+8>7+9>6+8,5+10>2+12>4+12;对沉降值的作用可分别表示为:2*≥1>Null,14+15≥17+18>7+8≥13+16>7+9>6+8,5+10>2+12>4+12。亚基组成类型共有24种,在蛋白质含量水平上,亚基组成1/14+15/5+10、1/14+15/2+12、1/7+8/5+10、1/17+18/5+10和1/7+9/5+10相对较高;在沉降值水平上,亚基组成1/14+15/5+10、1/17+18/5+10、1/7+8/5+10、2*/7+9/5+10和1/14+15/2+12相对较高。综上所述,在24种亚基组成类型中没有发现明显优势的组合类型,优质亚基2*、17+18和14+15出现的频率较低。江苏省中筋和弱筋小麦品种选育,应结合蛋白质含量、沉降值和亚基组成进行改良。
Abstract:
To figure out the genetic diversity of wheat cultivar(variety) from the northern region in China and provide the basic materials for wheat quality improvement, 172 accessions of wheat cultivar (variety) were analyzed by SDS-PAGE to reveal the alleles variance of high molecular weight gluten subunits (HWM-GS) and the relationship with protein content and sedimentation value. There were 12 alleles on Glu-1, 3 alleles on Glu-A1, 6 alleles on Glu-B1, and 3 alleles on Glu-D1. The frequencies of 1, 7+9, 5+10 alleles were the highest on Glu-A1,Glu-B1 and Glu-D1, being 54.07%, 48.26%, 49.42%, respectively. The effects of HMW-GS on three loci on protein content were presented as 1≥2*>Null, 13+16≥14+15≥17+18≥7+8>7+9>6+8, 5+10>2+12>4+12, and were listed in the order 2*≥1>Null, 14+15≥17+18>7+8≥13+16>7+9>6+8, 5+10>2+12>4+12 on sedimentation value. The protein contents with the subunit combinations of 1/14+15/5+10, 1/14+15/2+12, 1/7+8/5+10, 1/17+18/5+10 and 1/7+9/5+10 were higher. The sedimentation value were higher in the HMW-GS compositions of 1/14+15/5+10, 1/17+18/5+10, 1/7+8/5+10, 2*/7+9/5+10 and 1/14+15/2+12. No combination outperformed in quality traits among 24 types of subunit compositions, and high quality subunits 2*, 17 + 18 and 14 + 15 were rare in the tested varieties. The quality traits, such as protein content, sedimentation value, and high quality subunits, need to be taken into consideration in middle and weak gluten wheat variety improvement in Jiangsu province.

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备注/Memo

备注/Memo:
收稿日期:2014-12-04 基金项目:农业部小麦现代农业产业技术体系项目(CARS-03);江苏省农业科技自主创新基金项目[CX(12)2026];江苏省科技支撑项目(BE2013439) 作者简介:杨丹( 1980- ),女,黑龙江齐齐哈尔人,博士,副研究员,主要从事小麦品质分析。(Tel)15861815667;(E-mail)yangdan1290@163.com 通讯作者:姚金保,(Tel)025-84390298;(E-mail)yaojb@jaas.ac.cn 收稿日期:2014-08-02 基金项目:棉花生物学国家重点实验室开放课题(CB2014A02);中国博士后科学基金项目(2012M520473);国家自然科学基金项目(31460359);江苏省自然科学基金项目(BK20130429、BK20131204 );江苏省“六大人才高峰”项目(NY-34);公益性事业单位科研资助项目(KY2013056) 作者简介:王为(1980-),男,江苏泗阳人,博士,副研究员,从事棉花分子育种与种质创新研究工作。(E-mail) ww462@126.com。叶泗洪与王为具有同等贡献.。 通讯作者:王长彪,(E-mail)wcbkls@126.com
更新日期/Last Update: 2015-04-30