[1]陈龙,崔超,孙世君,等.小麦PDAT基因家族的鉴定及其对多重非生物胁迫的响应[J].江苏农业学报,2026,42(03):452-463.[doi:doi:10.3969/j.issn.1000-4440.2026.03.003]
　CHEN Long,CUI Chao,SUN Shijun,et al.Identification of the PDAT gene family in wheat and its response to multiple abiotic stresses[J].,2026,42(03):452-463.[doi:doi:10.3969/j.issn.1000-4440.2026.03.003]
点击复制

小麦PDAT基因家族的鉴定及其对多重非生物胁迫的响应()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
452-463
栏目:
遗传育种·生理生化
出版日期:
2026-03-31

文章信息/Info

Title:
Identification of the PDAT gene family in wheat and its response to multiple abiotic stresses
作者:
陈龙1; 2; 崔超1; 2; 孙世君1; 2; 杨东升1; 2; 王海伟1; 2; 卢倩倩1; 2; 杨秀娟1; 2; 郝水源1; 2
(1.河套学院农学系,内蒙古巴彦淖尔015000;2.河套地区绿色农产品安全生产与预警控制实验室,内蒙古巴彦淖尔015000)
Author(s):
CHEN Long1; 2; CUI Chao1; 2; SUN Shijun1; 2; YANG Dongsheng1; 2; WANG Haiwei1; 2; LU Qianqian1; 2; YANG Xiujuan1; 2; HAO Shuiyuan1; 2
(1.Agriculture Department, Hetao College, Bayannur 015000, China;2.Hetao Area Green Agricultural Product Safety Production and Early Warning Control Laboratory,Bayannur 015000,China)
关键词:
小麦; 磷脂二酰甘油酰基转移酶; 非生物胁迫; 表达模式
Keywords:
Triticum aestivum L.; phospholipid diacylglycerol acyltransferase(PDTA); abiotic stress; expression pattern
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.003
文献标志码:
A
摘要:
磷脂二酰甘油酰基转移酶(PDAT)是植物三酰甘油(TAG)合成的关键酶,参与调控TAG生物合成及非生物胁迫响应。为解析小麦PDAT基因家族的特征及表达规律,本研究依托小麦基因组数据库,针对TaPDAT基因家族成员开展蛋白质理化性质分析、染色体定位、基因结构剖析、系统进化关系构建,同时探究其在不同组织及胁迫条件下的表达特征。本研究通过生物信息学手段完成小麦全基因组范围内的家族成员鉴定,并借助小麦公共表达数据库结合实时荧光定量PCR(RT-qPCR)技术,解析该家族基因在小麦不同组织中及胁迫处理下的表达模式。结果显示,小麦基因组含3个TaPDAT成员(TaPDAT1a、TaPDAT1b和 TaPDAT1c),分别定位于5A、5D、5B染色体,亚细胞定位预测结果显示TaPDAT1a、TaPDAT1b和 TaPDAT1c均定位于线粒体；系统进化分析将PDAT划分为PDAT1、PDAT2 2个亚家族,其中TaPDAT1a、TaPDAT1b和 TaPDAT1c均含5个内含子,鉴定到8对同源基因的共线性事件,且PDAT蛋白保守结构域高度统一,包含 N 端跨膜区与C端卵磷脂胆固醇酰基转移酶(LCAT)催化域(Pfam 登录号:PF02450),并共享8个保守基序。这些特征不仅清晰揭示了家族的进化分化规律,更确证其隶属于LCAT超家族；启动子顺式元件分析结果表明,TaPDAT 受植物激素、逆境应答通路协同调控。基因表达模式分析结果显示,TaPDAT基因家族成员表达具有显著组织特异性和发育阶段动态性,聚类为2组:TaPDAT1a和TaPDAT1c在生殖生长期和营养生长期的穗中表达水平较高，而在生殖生长期根中表达水平较低；TaPDAT1b却在生殖生长期穗和籽粒中表达量较高，在营养生长期的穗中表达量最低。在非生物胁迫响应中,在磷饥饿胁迫下TaPDAT1b的相对表达量在根和叶中轻微下调；TaPDAT1a 在根和叶中的相对表达量基本不变；TaPDAT1c则在根中的相对表达量变化不显著。qPCR检测结果显示,TaPDAT1a在叶与茎中高表达,TaPDAT1b和TaPDAT1c相对表达量分别在茎和叶中最高。干旱胁迫下,TaPDAT1a和TaPDAT1b均在干旱胁迫12 h显著上调,TaPDAT1c初期受抑制,后期表达稳定。磷饥饿胁迫时,TaPDAT1a和TaPDAT1b相对表达量分别在6 h和3 h时达到峰值,TaPDAT1c在初期高表达,后期逐渐降低。本研究解析了小麦TaPDAT基因家族的进化保守性与表达特异性,明确其在油脂合成、逆境响应中的调控潜力,为深入挖掘基因功能及小麦抗逆遗传改良提供了理论支撑。
Abstract:
Phospholipid diacylglycerol acyltransferase (PDAT) is a key enzyme in plant triacylglycerol (TAG) synthesis, which is involved in regulating TAG biosynthesis and responses to abiotic stresses. To clarify the characteristics and expression patterns of the PDAT gene family in wheat, this study relied on wheat genome databases to conduct analyses of the physicochemical properties of proteins, chromosome localization, gene structure, construction of phylogenetic evolution relationships, and investigation of expression characteristics under different tissues and stress conditions for members of the TaPDAT family. Bioinformatics methods were employed to identify TaPDAT family members at the whole-genome level of wheat. Furthermore, combined with wheat public expression databases and real-time fluorescent quantitative PCR (qRT-PCR) technology, the expression patterns of this gene family in different tissue types under stress treatments were analyzed. The results showed that the wheat genome contained three TaPDAT members (TaPDAT1a/1b/1c), which were localized on chromosomes 5A, 5D, and 5B, respectively, with all subcellular localizations in mitochondria. Phylogenetic evolution analysis classified PDAT members into PDAT1 and PDAT2 subfamilies. TaPDAT1a/1b/1c genes contained five introns, with eight pairs of synteny events identified among homologous genes. PDAT proteins shared highly conserved domains—comprising an N-terminal transmembrane region and a C-terminal lecithin-cholesterol acyltransferase (LCAT) catalytic domain (Pfam accession number: PF02450),as well as eight conserved motifs. These features not only clearly revealed the evolutionary differentiation patterns of the family but also confirmed its membership in the LCAT enzyme superfamily. Analysis of promoter cis-acting elements indicated that TaPDAT were coordinately regulated by plant hormone and stress response pathways. Gene expression pattern analysis revealed that the TaPDAT gene family members exhibited significant tissue specificity and developmental stage dynamics, clustering into two groups: TaPDAT1a and TaPDAT1c showed high expression levels in spikes during both reproductive and vegetative stages, but showed low expression levels in roots during the reproductive stage, whereas TaPDAT1b exhibited high expression levels in spikes and grains during the reproductive stage, and the lowest expression level in spikes during the vegetative stage. In response to abiotic stresses, TaPDAT1b was slightly down-regulated in roots and leaves under phosphorus starvation stress; the expression of TaPDAT1a remained basically unchanged in roots and leaves; TaPDAT1c showed no significant variation in expression in roots. qPCR detection results demonstrated that TaPDAT1a was highly expressed in leaves and stems, while TaPDAT1b and TaPDAT1c exhibited the highest expression levels in stems and leaves, respectively. Under drought stress, both TaPDAT1a and TaPDAT1b were significantly up-regulated at 12 h, while TaPDAT1c expression was inhibited in the early stage and maintained stable in the later stage. Under phosphorus starvation stress, TaPDAT1a and TaPDAT1b reached their peak expression levels at six hours and three hours, respectively. TaPDAT1c was highly expressed in the early stage, and its expression gradually decreased thereafter. This study clarified the evolutionary conservation and expression specificity of the wheat TaPDAT gene family, identified its regulatory potential in lipid synthesis and stress responses, and provided theoretical support for in-depth exploration of gene functions and genetic improvement of stress resistance in wheat.

参考文献/References:

[1]KAUP M T, FROESE C D, THOMPSON J E. A role for diacylglycerol acyltransferase during leaf senescence[J]. Plant Physiology,2002,129(4):1616-1626.
[2]THEODOULOU F L, EASTMOND P J. Seed storage oil catabolism:a story of give and take[J]. Current Opinion in Plant Biology,2012,15(3):322-328.
[3]FAN J L, YAN C S, XU C C. Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis[J]. The Plant Journal,2013,76(6):930-942.
[4]SU T, LI X Z, YANG M Y, et al. Autophagy:an intracellular degradation pathway regulating plant survival and stress response[J]. Frontiers in Plant Science,2020,11:164.
[5]KENNEDY E P. Biosynthesis of complex lipids[J]. Federation Proceedings,1961,20:934-940.
[6]DAHLQVIST A, STAHL U, LENMAN M, et al. Phospholipid:diacylglycerol acyltransferase:an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants[J]. PNAS,2000,97(12):6487-6492.
[7]BATES P D, BROWSE J. The significance of different diacylgy-cerol synthesis pathways on plant oil composition and bioengineering[J]. Frontiers in Plant Science,2012,3:147.
[8]STHL U, CARLSSON A S, LENMAN M, et al. Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis[J]. Plant Physiology,2004,135(3):1324-1335.
[9]MHASKE V, BELDJILALI K, OHLROGGE J, et al. Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid:diacylglycerol transacylase gene (At5g13640)[J]. Plant Physiology and Biochemistry,2005,43(4):413-417.
[10]LI R Z, YU K S, HILDEBRAND D F. DGAT1,DGAT2 and PDAT expression in seeds and other tissues of epoxy and hydroxy fatty acid accumulating plants[J]. Lipids,2010,45(2):145-157.
[11]PAN X, SILOTO R M P, WICKRAMARATHNA A D, et al. Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissimum L.) seed catalyzing the selective production of trilinolenin[J]. The Journal of Biological Chemistry,2013,288(33):24173-24188.
[12]VAN ERP H, BATES P D, BURGAL J, et al. Castor phospholi-pid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis[J]. Plant Physiology,2011,155(2):683-693.
[13]张程,董帅飞,朱艺,等. 向日葵PDAT基因家族鉴定及其对油脂积累和非生物胁迫的响应[J]. 植物生理学报,2022,58(5):844-856.
[14]KIM H U, LEE K R, GO Y S, et al. Endoplasmic reticulum-located PDAT1-2 from castor bean enhances hydroxy fatty acid accumulation in transgenic plants[J]. Plant & Cell Physiology,2011,52(6):983-993.
[15]FAN J L, YAN C S, ROSTON R, et al. Arabidopsis lipins,PDAT1 acyltransferase,and SDP1 triacylglycerol lipase synergistically direct fatty acids toward β-oxidation,thereby maintaining membrane lipid homeostasis[J]. The Plant Cell,2014,26(10):4119-4134.
[16]YUAN L X, MAO X, ZHAO K, et al. Characterisation of phospholipid:diacylglycerol acyltransferases (PDAT) from Camelina sativa and their roles in stress responses[J]. Biology Open,2017,6(7):1024-1034.
[17]DEMSKI K, OSIEWSKA A, JASIENIECKA-GAZARKIEWICZ K, et al. Phospholipid:diacylglycerol Acyltransferase1 overexpre-ssion delays senescence and enhances post-heat and cold exposure fitness[J]. Frontiers in Plant Science,2020,11:611897.
[18]徐赫,潘丽娟,陈娜,等. 磷脂二酰甘油酰基转移酶(PDAT)基因的克隆与表达分析[J]. 花生学报,2018,47(4):33-40,54.
[19]ZHANG M, FAN J L, TAYLOR D C, et al. DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development[J]. The Plant Cell,2009,21(12):3885-3901.
[20]蒋赟,王秀东. 我国小麦产业发展现状问题及对策浅析[J]. 南方农业,2020,14(31):31-34,46.
[21]LI-BEISSON Y, SHORROSH B, BEISSON F, et al. Acyl-lipid metabolism[J]. The Arabidopsis Book,2013,11:e0161.
[22]CHEN C J, WU Y, LI J W, et al. TBtools-Ⅱ:a “one for all,all for one” bioinformatics platform for biological big-data mining[J]. Molecular Plant,2023,16(11):1733-1742.
[23]MISTRY J, CHUGURANSKY S, WILLIAMS L, et al. Pfam:the protein families database in 2021[J]. Nucleic Acids Research,2021,49(D1):D412-D419.
[24]LETUNIC I,KHEDKAR S, BORK P. SMART:recent updates,new developments and status in 2020[J]. Nucleic Acids Research,2021,49(D1):D458-D460.
[25]GASTEIGER E. Protein identification and analysis tools on the ExPASy server[M]. Totowa,NJ:Humana Press,2005:571-607.
[26]HORTON P, PARK K J, OBAYASHI T, et al. WoLF PSORT:protein localization predictor[J]. Nucleic Acids Research,2007,35:W585-W587.
[27]LIVAK K J , SCHMITTGEN T D L. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCt method[J]. Methods,2001,25(4):402-408.
[28]LIU X Y, OUYANG L L, ZHOU Z G. Phospholipid:diacylglycerol acyltransferase contributes to the conversion of membrane lipids into triacylglycerol in Myrmecia incisa during the nitrogen starvation stress[J]. Scientific Reports,2016,6:26610.
[29]ZANG X S, GENG X L, MA L, et al. A genome-wide analysis of the phospholipid:diacylglycerol acyltransferase gene family in Gossypium[J]. BMC Genomics,2019,20(1):402.
[30]田海莹,单雷,李新国,等. 花生PDAT基因家族的全基因组生物信息学分析[J]. 花生学报,2018,47(3):1-7,13.
[31]MUELLER S P, UNGER M, GUENDER L, et al. Phospholipid:diacylglycerol acyltransferase-mediated triacylglyerol synthesis augments basal thermotolerance[J]. Plant Physiology,2017,175(1):486-497.
[32]刘慧春,张加强,周江华,等. 牡丹PsGRP基因克隆及转基因拟南芥的耐涝性分析[J]. 植物生理学报,2021,57(2):373-384.
[33]迟凯威,宋扬,李升林,等. 蒺藜苜蓿LIM家族的全基因组鉴定和表达分析[J]. 植物生理学报,2021,57(5):1074-1086.
[34]王馨磊,朱思琪,田晓娜,等. 紫花苜蓿RALF基因家族的全基因组鉴定及盐胁迫下表达分析[J]. 江苏农业学报,2025,41(7):1260-1269.
[35]ATKINSON N J, URWIN P E. The interaction of plant biotic and abiotic stresses:from genes to the field[J]. Journal of Experimental Botany,2012,63(10):3523-3543.
[36]BOUDSOCQ M, SHEEN J. SnRK2 protein kinases: a hub of stress and hormone signaling in plants[J]. Trends in Plant Science,2013,18(6):301-310.
[37]VIRLOUVET L, TACONNAT L, RENOU J P, et al. Dynamic coordination of transcriptional responses during salt stress adaptation in Arabidopsis[J]. The Plant Journal,2011,66(5):853-867.

相似文献/References:

[1]伍 宏,朱昌华,夏 凯,等.叶面喷施激动素对小麦品种济麦22品质的影响[J].江苏农业学报,2016,(02):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
　WU Hong,ZHU Chang-hua,XIA Kai,et al.Effect of foliar application of kinetin on quality of Triticum aestivum L. Jimai 22[J].,2016,(03):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
[2]蒋正宁,别同德,赵仁惠,等.受条锈菌诱导的小麦丝氨酸苏氨酸激酶基因TaS/TK的克隆与表达[J].江苏农业学报,2016,(05):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
　JIANG Zheng-ning,BIE Tong-de,ZHAO Ren-hui,et al.Cloning and expression analysis of a Serine/Threonine protein kinase gene TaS/TK in wheat in response to stripe rust fungal infection[J].,2016,(03):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
[3]丁彬彬,张旭,吴磊,等.小麦3B 短臂染色体抗赤霉病主效 QTL 区域候选基因的表达[J].江苏农业学报,2017,(01):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
　DING Bin-bin,ZHANG Xu,WU Lei,et al.Expression of candidate genes on the region of a major QTL for the resistance to Fusarium head blight on the short arm of chromosome 3B in wheat[J].,2017,(03):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
[4]周淼平,姚金保,张鹏,等.小麦幼苗纹枯病抗性评价新方法[J].江苏农业学报,2017,(01):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
　ZHOU Miao-ping,YAO Jin-bao,ZHANG Peng,et al.New method for the resistance evaluation of wheat sharp eyespot in seedling[J].,2017,(03):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
[5]吴磊,姜朋,张瑜,等.苏麦3号小麦穗部病毒诱导的基因沉默（VIGS）体系的建立及验证[J].江苏农业学报,2017,(02):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
　WU Lei,JIANG Peng,ZHANG Yu,et al.Construction and validation of virus-induced gene silencing(VIGS) system in spike of wheat variety Sumai 3[J].,2017,(03):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
[6]邵继锋,陈荣府,董晓英,等.利用分根技术研究小麦铝磷交互作用[J].江苏农业学报,2016,(01):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
　SHAO Ji-feng,CHEN Rong-fu,DONG Xiao-ying,et al.Aluminum-phosphorus interaction in wheat grown in a split-root device[J].,2016,(03):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
[7]叶景秀.小麦籽粒蛋白质双向电泳体系的优化[J].江苏农业学报,2015,(05):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
　YE Jing-xiu.Optimization of two-dimensional electrophresis system for grain protein in spring wheat[J].,2015,(03):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
[8]郑舒文,徐其隆,邹华文.脱落酸对涝渍胁迫下小麦产量的影响[J].江苏农业学报,2015,(05):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
　ZHENG Shu-wen,XU Qi-long,ZOU Hua-wen.Yield of waterlogged wheat in response to ABA application[J].,2015,(03):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
[9]张玉萍,马占鸿.不同施氮量下小麦遥感估产模型构建[J].江苏农业学报,2015,(06):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
　ZHANG Yu-ping,MA Zhan-hong.Yield estimation model of wheat based on remote sensing data under different nitrogen supply conditions[J].,2015,(03):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
[10]张卓亚,王晓琳,许晓明,等.腐植酸对小麦扬花期水分利用效率及灌浆进程的影响[J].江苏农业学报,2015,(04):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]
　ZHANG Zhuo-ya,WANG Xiao-ling,XU Xiao-ming,et al.Effect of humic acid on water use efficiency and grouting process of wheat at flowering[J].,2015,(03):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]

备注/Memo

备注/Memo:
收稿日期:2025-08-25基金项目:“科技兴蒙”行动重点专项(NMKJXM202201)作者简介:陈龙(1988-),男,陕西汉中人,博士,副教授,研究方向为分子生物学与基因组学。(E-mail)15774711140@163.com通讯作者:郝水源,(E-mail)1766094979@qq.com
更新日期/Last Update: 2026-04-17