[1]宋佳敏,何佳伟,蒋伟勤,等.喷施水杨酸和褪黑素对药隔期低温胁迫下小麦产量及生理特性的影响[J].江苏农业学报,2025,(03):477-484.[doi:doi:10.3969/j.issn.1000-4440.2025.03.006]
　SONG Jiamin,HE Jiawei,JIANG Weiqin,et al.Effects of salicylic acid and melatonin on yield and physiological characteristics of wheat under low temperature stress at anther connective stage[J].,2025,(03):477-484.[doi:doi:10.3969/j.issn.1000-4440.2025.03.006]
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喷施水杨酸和褪黑素对药隔期低温胁迫下小麦产量及生理特性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年03期
页码:
477-484
栏目:
遗传育种·生理生化
出版日期:
2025-03-31

文章信息/Info

Title:
Effects of salicylic acid and melatonin on yield and physiological characteristics of wheat under low temperature stress at anther connective stage
作者:
宋佳敏1; 何佳伟1; 蒋伟勤1; 车阳1; 李其胜1; 文廷刚1; 顾大路1; 杜小凤1; 顾克军2; 杨威3
(1.江苏徐淮地区淮阴农业科学研究所,江苏淮安223001；2.江苏省农业科学院,江苏南京210014；3.淮阴师范学院,江苏淮安223300)
Author(s):
SONG Jiamin1; HE Jiawei1; JIANG Weiqin1; CHE Yang1; LI Qisheng1; WEN Tinggang1; GU Dalu1; DU Xiaofeng1; GU Kejun2; YANG Wei3
(1.Huaiyin Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Huai’an 223001, China;2.Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Huaiyin Normal University, Huai’an 223300, China)
关键词:
小麦; 倒春寒; 药隔期; 水杨酸; 褪黑素; 产量; 生理特性
Keywords:
wheat; late spring cold; anther connective stage; salicylic acid; melatonin; yield; physiological characteristics
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.03.006
文献标志码:
A
摘要:
为探明药隔期低温胁迫前喷施水杨酸和褪黑素对小麦光合作用、抗氧化特性及产量的影响,以淮麦40为材料开展盆栽试验。试验于药隔期人工模拟倒春寒(昼夜温度设置为2 ℃ 12 h /-2 ℃ 12 h),在低温胁迫前喷施300 mg/L水杨酸(SA处理)和46 mg/L褪黑素(MT处理),并以低温+清水(CL处理)、常温+清水(CK)作为对照,调查分析小麦产量及其生理特性的变化情况。结果表明,相比于CL处理,喷施水杨酸和褪黑素显著提高了叶片中超氧化物歧化酶（SOD）、过氧化物酶（POD）和抗坏血酸过氧化物酶（APX）3种抗氧化酶的活性,并保持了较高的还原型谷胱甘肽（GSH）浓度与氧化型谷胱甘肽（GSSG）浓度的比值,使丙二醛（MDA）含量显著下降。这有效缓解了低温对光合作用的抑制作用,故叶绿素相对含量（SPAD）和地上部干物质积累显著提高,有效减轻了低温对产量造成的不利影响,其中相较CL处理,MT处理、SA处理的小麦产量显著提高。同时,喷施水杨酸和褪黑素处理也缓解了低温对穗分化发育的不利影响,其穗粒数和穗数协同提高,而相较于CK,穗数、穗粒数和千粒重均未发生显著变化,其中以SA处理的效果更好。以上结果表明,药隔期低温胁迫前喷施水杨酸和褪黑素均能通过调控小麦体内抗氧化系统酶活性来增强小麦倒春寒抗性,保证小麦的正常生长发育。
Abstract:
To investigate the effects of spraying salicylic acid and melatonin before low temperature stress during the anther connective stage on photosynthesis, antioxidant characteristics and yield of wheat, a pot experiment was conducted using Huaimai 40 as the material. The experiment artificially simulated late spring cold during the anther connective stage (day and night temperature was set at 2 ℃ 12 h /-2 ℃ 12 h), sprayed 300 mg/L salicylic acid (SA) and 46 mg/L melatonin (MT) before low temperature stress, and used low temperature + clean water (CL) and normal temperature + clean water (CK) as controls to investigate and analyze the changes in wheat yield and physiological characteristics. The results showed that compared with the CL treatment, spraying SA and MT significantly increased the activities of three antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX), and maintained a higher ratio of reduced glutathione (GSH) concentration to oxidized glutathione (GSSG) concentration in leaves, leading to a significant decrease in malondialdehyde (MDA) content. This effectively alleviated the inhibitory effect of low temperature on photosynthesis, so the relative chlorophyll content (SPAD value) and aboveground dry matter accumulation were significantly increased, which effectively mitigated the adverse effects of low temperature on yield. Compared with CL, the wheat yield was significantly increased in MT and SA. At the same time, the spraying treatment also alleviated the adverse effects of low temperature on spike differentiation and development, and the kernels per spike and spikes increased synergistically. Compared with CK, the spikes, kernels per spike and the 1 000-kernel weight did not change significantly. Among them, SA had the best effect. The above results show that spraying salicylic acid and melatonin before low temperature stress during the anther connective stage can enhance the resistance of wheat to late spring cold by regulating the activities of antioxidant enzymes in wheat, and ensure the normal growth and development of wheat.

参考文献/References:

[1]刘录祥. 我国小麦产业科技创新发展现状与展望[J]. 寒旱农业科学,2024,3(6):491-494.
[2]YUE Y J, ZHOU Y, WANG J A, et al. Assessing wheat frost risk with the support of GIS:an approach coupling a growing season meteorological index and a hybrid fuzzy neural network model[J]. Sustainability,2016,8(12):1308.
[3]钟秀丽,王道龙,赵鹏,等. 黄淮麦区小麦拔节后霜冻的农业气候区划[J]. 中国生态农业学报,2008,16(1):11-15.
[4]刘畅,王维领,赵灿,等. 小麦倒春寒研究现状及展望[J]. 江苏农业学报,2022,38(4):1115-1122.
[5]方宇辉,齐学礼,张煜,等. 黄淮麦区小麦品种抗倒春寒鉴定及其评价方法构建[J]. 麦类作物学报,2023,43(10):1288-1297.
[6]李春燕,徐雯,刘立伟,等. 药隔至开花期低温对小麦产量和生理特性的影响[J]. 麦类作物学报,2016,36(1):77-85.
[7]RIVAS-SAN VICENTE M, PLASENCIA J. Salicylic acid beyond defence:its role in plant growth and development[J]. Journal of Experimental Botany,2011,62(10):3321-3338.
[8]王维领,赵灿,李国辉,等. 水杨酸在植物抵御低温胁迫中的作用[J]. 植物生理学报,2020,56(12):2585-2594.
[9]WANG W L, WANG X, HUANG M, et al. Hydrogen peroxide and abscisic acid mediate salicylic acid-induced freezing tolerance in wheat[J]. Frontiers in Plant Science,2018,9:1137.
[10]BACK K, TAN D X, REITER R J. Melatonin biosynthesis in plants:multiple pathways catalyze tryptophan to melatonin in the cytoplasm or chloroplasts[J]. Journal of Pineal Research,2016,61(4):426-437.
[11]ARNAO M B, HERNNDEZ-RUIZ J. Melatonin in flowering,fruit set and fruit ripening[J]. Plant Reproduction,2020,33(2):77-87.
[12]TURK H, ERDAL S, GENISEL M, et al. The regulatory effect of melatonin on physiological,biochemical and molecular parameters in cold-stressed wheat seedlings[J]. Plant Growth Regulation,2014,74(2):139-152.
[13]WANG Y P, REITER R J, CHAN Z L. Phytomelatonin:a universal abiotic stress regulator[J]. Journal of Experimental Botany,2018,69(5):963-974.
[14]李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000.
[15]DU Z Y, BRAMLAGE W J. Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts[J]. Journal of Agricultural and Food Chemistry,1992,40(9):1566-1570.
[16]HASANUZZAMAN M, NAHAR K, ANEE T I, et al. Glutathione in plants:biosynthesis and physiological role in environmental stress tolerance[J]. Physiology and Molecular Biology of Plants,2017,23(2):249-268.
[17]WEI L T, WANG L N, YANG Y, et al. Abscisic acid enhances tolerance of wheat seedlings to drought and regulates transcript levels of genes encoding ascorbate-glutathione biosynthesis[J]. Frontiers in Plant Science,2015,6:458.
[18]XIAO L J, LIU L L, ASSENG S, et al. Estimating spring frost and its impact on yield across winter wheat in China[J]. Agricultural and Forest Meteorology,2018,260:154-164.
[19]张军,鲁敏,孙树贵,等. 拔节期低温胁迫对小麦生理生化特性和产量的影响[J]. 西北农业学报,2014,23(2):73-79.
[20]苏慧,李金鹏,胡燕美,等. 喷施KH2PO4对孕穗期低温胁迫下小麦叶片抗氧化特性与幼穗冻害的影响[J]. 麦类作物学报,2021,41(5):585-593.
[21]JUNG K H, HUR J, RYU C H, et al. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system[J]. Plant and Cell Physiology,2003,44(5):463-472.
[22]马泉,张玉雪,陶源,等. 春季不同时期低温对小麦光合特性和粒重形成的影响[J]. 麦类作物学报,2022,42(2):226-235.
[23]柯媛媛,陈翔,张乐乐,等. 药隔期低温胁迫对小麦干物质积累、转运和分配及产量的影响[J]. 安徽农业大学学报,2021,48(5):701-706.
[24]高芸,张玉雪,马泉,等. 春季低温对小麦花粉育性及粒数形成的影响[J]. 作物学报,2021,47(1):104-115.
[25]JI H T, XIAO L J, XIA Y M, et al. Effects of jointing and booting low temperature stresses on grain yield and yield components in wheat[J]. Agricultural and Forest Meteorology,2017,243:33-42.
[26]张雪莲,罗德旭,杨红,等. 外源褪黑素和硒对高温胁迫下辣椒生理特性和抗氧化系统的影响[J]. 江苏农业学报,2023,39(8):1729-1738.
[27]赵欣,孟德义,苍晶,等. 外源SA对低温胁迫下冬小麦糖酵解代谢的影响[J]. 麦类作物学报,2022,42(8):1031-1038.
[28]杜思梦,方保停,李向东,等. 外源水杨酸对低温胁迫下小麦幼苗叶绿素荧光特性及抗氧化酶活性的影响[J]. 江苏农业科学,2022,50(19):68-73.
[29]朱春权,魏倩倩,项兴佳,等. 褪黑素和茉莉酸甲酯基质育秧对水稻耐低温胁迫的调控作用[J]. 作物学报,2022,48(8):2016-2027.
[30]SCEBBA F, SEBASTIANI L, VITAGLIANO C. Changes in activity of antioxidative enzymes in wheat (Triticum aestivum) seedlings under cold acclimation[J]. Physiologia Plantarum,1998,104(4):747-752.
[31] CAVERZAN A, CASASSOLA A, BRAMMER S P. Antioxidant responses of wheat plants under stress[J]. Genetics and Molecular Biology,2016,39(1):1-6.
[32]赵海亮,左璐,张璐,等. 低温胁迫下外源褪黑素对番茄幼苗光抑制的缓解效应[J]. 应用生态学报,2023,34(1):151-159.
[33]薛盈文,黄寿光,范博文,等. 低温和UV-B复合胁迫对小麦幼苗抗氧化酶和渗透调节物质的影响[J]. 麦类作物学报,2017,37(6):834-840.
[34]周艳,刘慧英,邓嘉欣,等. GSH/GSSG对盐胁迫下番茄幼苗谷胱甘肽化修饰和抗氧化系统的影响[J]. 分子植物育种,2021,19(6):1995-2003.
[35]李璇,岳红,王升,等. 影响植物抗氧化酶活性的因素及其研究热点和现状[J]. 中国中药杂志,2013,38(7):973-978.
[36]葛君,姜晓君,任德超,等. 低温胁迫对拔节期小麦抗氧化系统及光合能力的影响[J]. 天津农业科学,2021,27(9):5-9.

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备注/Memo

备注/Memo:
收稿日期：2024-10-14基金项目：江苏省农业科技自主创新基金项目[CX(22)1002];淮安市农业科学研究院科研发展基金项目(HABL202227)；国家现代农业产业技术体系专项(CARS-03)作者简介：宋佳敏(1996-),女,山西阳泉人,硕士,研究实习员,主要研究方向为小麦化控栽培技术。(E-mail)962193633@qq.com通讯作者：杜小凤,(E-mail)15061234456@163.com；顾大路,(E-mail)1302680742@qq.com
更新日期/Last Update: 2025-04-27