[1]张高原,魏兵强.西瓜隐性核雄性不育相关基因筛选及定位[J].江苏农业学报,2024,(06):1089-1097.[doi:doi:10.3969/j.issn.1000-4440.2024.06.016]
　ZHANG Gaoyuan,WEI Bingqiang.Screening and mapping of genes related to recessive nuclear male sterility in watermelon[J].,2024,(06):1089-1097.[doi:doi:10.3969/j.issn.1000-4440.2024.06.016]
点击复制

西瓜隐性核雄性不育相关基因筛选及定位()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年06期
页码:
1089-1097
栏目:
园艺
出版日期:
2024-06-30

文章信息/Info

Title:
Screening and mapping of genes related to recessive nuclear male sterility in watermelon
作者:
张高原; 魏兵强
(甘肃农业大学园艺学院,甘肃兰州730070)
Author(s):
ZHANG Gaoyuan; WEI Bingqiang
(College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China)
关键词:
西瓜; 雄性不育; 育性相关基因; 基因定位
Keywords:
watermelon; male sterility; fertility related genes; gene mapping
分类号:
S651
DOI:
doi:10.3969/j.issn.1000-4440.2024.06.016
摘要:
为挖掘更多的西瓜花药、花粉发育相关基因,解析其调控网络,本研究以西瓜雄性不育植株和可育植株为材料,构建遗传分离群体,并利用BSA-seq和RNA-seq进行基因定位和育性相关基因筛选。结果表明,雄性不育性状由单个隐性细胞核基因控制,该基因被定位在6号染色体的5 766 829 bp至21 366 400 bp之间。结合SNP/InDel变异位点、基因注释及表达分析,获得1个关键候选差异表达基因Cla97C06G117840以及受该基因表达影响的20个其他差异表达基因。生物信息学分析推测Cla97C06G117840由于部分编码序列缺失导致编码蛋白质提前终止,进而引起花粉败育。此外20个其他差异表达基因被富集在花药壁绒毡层发育和花粉壁组件基因集通路中,可能形成基因调控网络来影响花药、花粉发育。本研究为后期基因功能验证奠定了基础。
Abstract:
To explore more genes related to watermelon anther and pollen development and analyze its regulatory network, the genetic segregation population was constructed using watermelon male sterile materials and male fertile materials. And the gene mapping and fertility related gene screening were carried out by using BSA-seq and RNA-seq. The results showed that male sterility trait was controlled by a single recessive nuclear gene, which was mapped between 5 766 829 bp and 21 366 400 bp on chromosome 6. In combination with SNP/InDel variation site, gene annotation and expression analysis, one key candidate differentially expressed gene Cla97C06G117840 and 20 other differentially expressed genes affected by the expression of this gene were obtained. Bioinformatics analysis suggested that the deletion of some coding sequences of Cla97C06G117840 led to premature termination of the coding protein, resulting in pollen abortion. In addition, 20 other differentially expressed genes were enriched in the gene pathway of anther wall tapetum development and pollen wall assembly. These genes might form a gene regulatory network to affect anther and pollen development. This study can lay a foundation for later gene function verification.

参考文献/References:

[1]张显,王鸣,马建祥,等. 西瓜隐性核型雄性不育两用系的细胞学研究[J]. 西北农林科技大学学报(自然科学版),2005,33(1):71-74.
[2]陈雨,张建农,刘炬. 西瓜雄性不育花芽显微结构观察[J]. 甘肃农业大学学报,2008,43(5):67-70.
[3]马建祥,张显,张 勇,等. 西瓜雄性不育两用系雄蕊电镜扫描观察[J]. 北方园艺,2011,(3):151-152.
[4]王永琦,杨小振,莫言玲,等. 西瓜雄性不育系‘Se18’抗氧化酶活性和内源激素含量变化分析[J]. 园艺学报,2016,43(11):2161-2172.
[5]刘海河,侯喜林,张彦萍. 西瓜核雄性不育育性基因的RAPD标记[J]. 果树学报,2004,21(5):491-493.
[6]马建祥,张显,张勇,等. 西瓜核型雄性不育两用系POD同工酶研究[J]. 中国瓜菜,2005(4):30-32.
[7]郭守鹏,刘海河,张彦萍,等. 西瓜核雄性不育两用系G17AB育性基因的AFLP分子标记[J]. 园艺学报,2009,36(3):427-430.
[8]张彦萍,刘海河,谢彬,等. 西瓜细胞核雄性不育系雄花蕾的mRNA差异表达分析[J]. 果树学报,2010,27(6):1037-1041.
[9]于远,张显,张勇,等. mRNA差异显示分离西瓜核雄性不育基因的相关cDNA片段[J]. 西北农林科技大学学报(自然科学版),2008,36(11):162-166.
[10]RHEE S J, SEO M, JANG Y J, et al. Transcriptome profiling of differentially expressed genes in floral buds and flowers of male sterile and fertile lines in watermelon[J]. BMC Genomics,2015,16:914.
[11]DONG W, WU D, YAN C, et al. Mapping and analysis of a novel genic male sterility gene in watermelon (Citrullus lanatus)[J]. Frontiers in Plant Science,2021,12:639431.
[12]ZHU Y, YUAN G, JIA S, et al. Transcriptomic profiling of watermelon (Citrullus lanatus) provides insights into male flowers development[J]. Journal of Integrative Agriculture,2022,21(2):407-421.
[13]ZHANG G, DING Q, WEI B, et al. Transcriptome analysis of sterile and fertile floral buds from recessive genetic male sterility lines in watermelon (Citrullus lanatus L.)[J]. Journal of Plant Growth Regulation,2023,42:2800-2812.
[14]CHEN S, ZHOU Y, CHEN Y, et al. Fastp:an ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics,2018,34(17):884-890.
[15]LI H, DURBIN R. Fast and accurate short read alignment with Burrows-Wheeler transform[J]. Bioinformatics,2009,25(14):1754-1760.
[16]LI H, HANDSAKER B, WYSOKER A, et al. The sequence alignment/map format and SAMtools[J]. Bioinformatics,2009,25(16):2078-2079.
[17]MCKENNA A, HANNA M, BANKS E, et al. The genome analysis toolkit:a MapReduce framework for analyzing next-generation DNA sequencing data[J]. Genome Research,2010,20(9):1297-1303.
[18]WANG K, LI M, HAKONARSON H. ANNOVAR:functional annotation of genetic variants from high-throughput sequencing data[J]. Nucleic Acids Research,2010,38(16):e164.
[19]EMMS D M, KELLY S. OrthoFinder:phylogenetic orthology inference for comparative genomics[J]. Genome Biology,2019,20(1):238.
[20]SHANNON P, MARKIEL A, OZIER O, et al. Cytoscape:a software environment for integrated models of biomolecular interaction networks[J]. Genome Research,2003,13(11):2498-2504.
[21]FU Y, LI M, ZHANG S, et al. Analyses of functional conservation and divergence reveal requirement of bHLH010/089/091 for pollen development at elevated temperature in Arabidopsis[J]. Journal of Genetics and Genomics,2020,47(8):477-492.
[22]ZHU J, CHEN H, LI H, et al. Defective in tapetal development and function 1 is essential for anther development and tapetal function for microspore maturation in Arabidopsis[J]. The Plant Journal,2008,55(2):266-277.
[23]FERGUSON A C, PEARCE S, BAND L R, et al. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis[J]. The New Phytologist,2017,213(2):778-790.
[24]PHAN H A, IACUONE S, LI S F, et al. The MYB80 transcription factor is required for pollen development and the regulation of tapetal programmed cell death in Arabidopsis thaliana[J]. Plant Cell,2011,23:2209-2224.
[25]YANG C, VIZCAY-BARRENA G, CONNER K, et al. MALE STERILITY1 is required for tapetal development and pollen wall biosynthesis[J]. Plant Cell,2007,19:3530-3548.
[26]马伯军,刘程捷,陈析丰,等. 花粉壁发育相关基因的研究进展[J]. 浙江师范大学学报(自然科学版),2013,36(4):438-443.
[27]DONG X, HONG Z, SIVARAMAKRISHNAN M, et al. Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis[J]. The Plant Journal,2005,42(3):315-328.
[28]YANG J, TIAN L, SUN M X, et al. AUXIN RESPONSE FACTOR17 is essential for pollen wall pattern formation in Arabidopsis[J]. Plant Physiology,2013,162(2):720-731.
[29]DOBRITSA A A, NISHIKAWA S, PREUSS D, et al. LAP3,a novel plant protein required for pollen development, is essential for proper exine formation[J]. Sex Plant Reproduction,2009,22(3):167-177.
[30]QUILICHINI T D, GRIENENBERGER E, DOUGLAS C J. The biosynthesis,composition and assembly of the outer pollen wall:a tough case to crack[J]. Phytochemistry,2015,113:170-182.
[31]RHEE S Y, OSBORNE E, POINDEXTER P D, et al. Microspore separation in the quartet 3 mutants of Arabidopsis is impaired by a defect in a developmentally regulated polygalacturonase required for pollen mother cell wall degradation[J]. Plant Physiology,2003,133(3):1170-1180.

相似文献/References:

[1]王夏雯,余翔,乔俊卿,等.西瓜茬后种植稻麦对土壤微生物数量和西瓜枯萎病发生的影响[J].江苏农业学报,2015,(06):1291.[doi:doi:10.3969/j.issn.1000-4440.2015.06.015]
　WANG Xia-wen,YU Xiang,QIAO Jun-qing,et al.Effect of rice-wheat rotation after watermelon season on the amount of soil microbes and the incidence of Fusarium wilt[J].,2015,(06):1291.[doi:doi:10.3969/j.issn.1000-4440.2015.06.015]
[2]王玮,汪国莲,梁双林,等.叶面喷施氨基多糖硒肥对西瓜生长及产量品质的影响[J].江苏农业学报,2019,(06):1413.[doi:doi:10.3969/j.issn.1000-4440.2019.06.021]
　WANG Wei,WANG Guo-lian,LIANG Shuang-lin,et al.Effects of selenium-aminopolysaccharide fertilizer on growth, yield and quality of watermelon[J].,2019,(06):1413.[doi:doi:10.3969/j.issn.1000-4440.2019.06.021]
[3]徐彦刚,姚协丰,李良俊,等.西瓜品种（品系）对蔓枯病的抗性鉴定与评价[J].江苏农业学报,2021,(01):106.[doi:doi:10.3969/j.issn.1000-4440.2021.01.014]
　XU Yan-gang,YAO Xie-feng,LI Liang-jun,et al.Identification and evaluation of the resistance to gummy stem blight caused by Stagonosporopsis citrulli of some watermelon varieties (strains)[J].,2021,(06):106.[doi:doi:10.3969/j.issn.1000-4440.2021.01.014]
[4]孟佳丽,吴绍军,沈虹,等.不同药剂处理对西瓜连作土壤的影响[J].江苏农业学报,2021,(03):651.[doi:doi:10.3969/j.issn.1000-4440.2021.03.013]
　MENG Jia-li,WU Shao-jun,SHEN Hong,et al.Effects of different chemical treatments on continuous cropping soil of watermelon[J].,2021,(06):651.[doi:doi:10.3969/j.issn.1000-4440.2021.03.013]
[5]刘欣,程瑞,徐兵划,等.基于KASP技术的SNP标记用于西瓜品种指纹图谱构建和种子纯度检测[J].江苏农业学报,2022,38(05):1348.[doi:doi:10.3969/j.issn.1000-4440.2022.05.022]
　LIU Xin,CHENG Rui,XU Bing-hua,et al.Application of SNP markers based on KASP technology in fingerprint construction and seed purity detection of watermelon varieties[J].,2022,38(06):1348.[doi:doi:10.3969/j.issn.1000-4440.2022.05.022]
[6]张朝阳,程瑞,徐兵划,等.BSA联合转录组分析发掘西瓜叶片黄化候选基因[J].江苏农业学报,2024,(01):165.[doi:doi:10.3969/j.issn.1000-4440.2024.01.018]
　ZHANG Chao-yang,CHENG Rui,XU Bing-hua,et al.Identification of candidate genes for watermelon leaf yellowing based on BSA and transcriptome analysis[J].,2024,(06):165.[doi:doi:10.3969/j.issn.1000-4440.2024.01.018]

备注/Memo

备注/Memo:
收稿日期:2023-05-06基金项目:甘肃省教育厅创新基金项目(2022B-102)；甘肃农业大学省部共建干旱生境作物学国家重点实验室开放基金项目(GSCS-2020-09)；甘肃农业大学公开招聘博士科研启动基金项目(2017RCZX-30)作者简介:张高原 (1987-),男,河南漯河人,博士,讲师,研究方向为西瓜育种及分子生物学。(E-mail)zhanggy@gsau.edu.cn
更新日期/Last Update: 2024-07-15