[1]李萌楠,乐秀虎,周阳,等.二氢卟吩铁调控辣椒对高温胁迫的耐性及相关基因的表达[J].江苏农业学报,2024,(06):1070-1077.[doi:doi:10.3969/j.issn.1000-4440.2024.06.014]
 LI Mengnan,LE Xiuhu,ZHOU Yang,et al.Regulation of iron chlorine e6 on high temperature stress tolerance and related gene expression in pepper[J].,2024,(06):1070-1077.[doi:doi:10.3969/j.issn.1000-4440.2024.06.014]
点击复制

二氢卟吩铁调控辣椒对高温胁迫的耐性及相关基因的表达()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年06期
页码:
1070-1077
栏目:
园艺
出版日期:
2024-06-30

文章信息/Info

Title:
Regulation of iron chlorine e6 on high temperature stress tolerance and related gene expression in pepper
作者:
李萌楠12乐秀虎1周阳3季英华2魏利辉2周冬梅2谢加飞4
(1.河北工程大学园林与生态工程学院,河北邯郸471023;2.江苏省农业科学院植物保护研究所,江苏南京210014;3.全国农业技术推广服务中心,北京100125;4.高邮市农业农村局,江苏高邮225600)
Author(s):
LI Mengnan12LE Xiuhu1ZHOU Yang3JI Yinghua2WEI Lihui2ZHOU Dongmei2XIE Jiafei4
(1.School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 471023, China;2.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.National Agro-Tech Extension and Service Center, Beijing 100125, China;4.Gaoyou Agricultural and Rural Bureau, Gaoyou225600, China)
关键词:
辣椒二氢卟吩铁高温胁迫耐高温胁迫基因
Keywords:
pepperiron chlorine e6high-temperature stressheat tolerance-related genes
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2024.06.014
摘要:
为评估高温胁迫下二氢卟吩铁(Iron chlorine e6,ICE6)缓解辣椒幼苗高温伤害效应及对耐高温胁迫相关基因表达量的影响。本研究以艳椒465为供试品种,探究高温胁迫下,二氢卟吩铁对辣椒幼苗相对电导率、可溶性糖含量、抗氧化酶活性、脯氨酸含量等生理生化指标和耐高温相关基因表达的影响。试验结果表明,高温胁迫下,与清水对照比,喷施0.2 μg/ml二氢卟吩铁可提高辣椒叶片中可溶性糖、脯氨酸、还原型谷胱甘肽含量,增强超氧化物歧化酶、过氧化物酶活性,降低相对电导率及丙二醛含量;二氢卟吩铁能提高CaWRKY19、CaWRKY55和CaWRKY40耐高温胁迫相关基因的表达。由此可见,用0.2 μg/ml二氢卟吩铁处理可显著提高辣椒耐高温胁迫能力。
Abstract:
The purpose of this study was to evaluate the effects of iron chlorine e6 (ICE6) on alleviating high temperature damage and the expression of heat tolerance-related genes in pepper seedlings under high temperature stress. In this study, Yanjiao 465 was used as the test variety to explore the effects of iron chlorine e6 on physiological and biochemical indices such as relative conductivity, soluble sugar content, antioxidant enzyme activity, proline content and the expression of heat tolerance-related genes in pepper seedlings under high temperature stress. The results showed that under high temperature stress, compared with the control, spraying 0.2 μg/ml iron chlorine e6 could increase the contents of soluble sugar, proline and reduced glutathione in pepper leaves, enhance the activities of superoxide dismutase and peroxidase, and reduce the relative conductivity and malondialdehyde content. Iron chlorine e6 could increase the expression of heat tolerance-related genes CaWRKY19, CaWRKY55 and CaWRKY40. Therefore, the treatment with 0.2 μg/ml iron chlorine e6 could significantly improve the high temperature resistance of pepper.

参考文献/References:

[1]HATFIELD J L, PRUEGER J H. Temperature extremes:effect on plant growth and development[J]. Weather Clim Extrem,2015,10(PartA):4-10.
[2]TALUKDAR D. Biocomputation assisted data base of plant stress responsive traits:current progress and future challenges[J]. Tropical Plant Research,2015,2(2):143-152.
[3]王立浩,张宝玺,张正海,等. “十三五”我国辣椒育种研究进展产业、现状及展望[J]. 中国蔬菜,2021(2):21-29.
[4]梁宝萍,段莹,姜俊,等. 高温胁迫对辣椒果实活性氧代谢的影响[J]. 陕西农业科学,2022,68(8):93-96,102.
[5]朱长志,张志仙,檀国印,等. 蔬菜作物高温胁迫研究进展[J]. 江西农业学报,2017,29(2):53-57.
[6]MUNNE-BOSCH S. Drought-induced changes in the redox state of alpha-tocopherol,ascorbate,and the diterpene carnosic acid in chloroplasts of labiatae species differing in carnosic acid contents [J]. Plant Physiology,2003,131(4):1816-1825.
[7]刘强,张贵友,陈受宜. 植物转录因子的结构与调控作用[J].科学通报,2000,45(14):1465-1474.
[8]EULGEM T, RUSHTON P J, ROBATZEK S, et al. The WRKY superfamily of plant transcription factors [J]. Trends in Plant Science,2000(5):199-206.
[9]RUSHTON P J, SOMSSICH I E, RINGLER P, et al. WRKY transcription factors [J]. Trends in Plant Science,2010,15(5):247-258.
[10]张蓓,任福森,赵洋,等. 辣椒响应热胁迫机制的研究进展[J]. 生物技术通报,2023,39(7):37-47.
[11]彭新盛. 叶面喷施植物生长调节剂对油茶盐胁迫适应性的影响[J]. 特种经济动植物,2023,26(4):43-45.
[12]冯雪锋,刘慧聪,许雷,等. 植物生长调节剂在园艺植物中的应用[J]. 农业科技与信息,2023(3):122-125.
[13]吴梦丹,叶旭,魏大勇,等. 生长调节剂缓解樱桃萝卜盐胁迫的生理效应[J]. 江西农业学报,2023,35(2):89-95.
[14]陈黎明. 植物生长调节剂二氢卟吩铁[J]. 农药科学与管理,2018,39(3):67-68.
[15]王一凡,张列峰,蒋慧,等. 不同肥力条件下小麦施用叶绿酸铁的效果[J]. 江苏农业科学,2012,40(6):78-79.
[16]陈嘉敏,刘瑞显,张国伟,等. 二氢卟吩铁对花针期干旱及复水后花生叶片光合特性的影响[J]. 花生学报,2023,52(1):52-62.
[17]姜燕,黄新根,杨寅桂,等. 辣椒苗期耐热性及其生理生化特性研究[J]. 长江蔬菜,2014(12):47-50.
[18]曹存凤. 叶面喷施DHFe对大豆盐害缓解效应及其机理研究[D]. 南京:南京农业大学,2016.
[19]李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000.
[20]陈晓远,凌木生,高志红. 水分胁迫对水稻叶片可溶性糖和游离脯氨酸含量的影响[J]. 河南农业科学,2006(12):26-30.
[21]SHENG Y, WEI C, LEI S, et al. A CaCDPK29-CaWRKY27b module promotes CaWRKY40-mediated thermotolerance and immunity to Ralstonia solanacearum in pepper[J]. New Phytologist,2021(4):1843-1863.
[22]王学府,曹秋芬. 外源NO对干旱胁迫下辣椒幼苗生理影响及相关基因表达[J]. 分子植物育种,2022,20(5):1651-1657.
[23]李易初,石凤梅,马立功,等. 核盘菌菌丝内POD和SOD活性与其对大豆致病力关系初探[J]. 黑龙江八一农垦大学学报,2021,33(1):1-6,14.
[24]徐海,宋波,顾宗福,等. 植物耐热机理研究进展[J]. 江苏农业学报,2020,36(1):243-250.
[25]夏莹莹,叶航,马锦林,等. 4个油茶品种的半致死温度与耐热性研究[J]. 中国农学通报,2012,28(4):58-61.
[26]朱德宁,刘素娜,赵绮薇,等. 有棱丝瓜耐热性种质资源筛选与耐热生理响应[J]. 江苏农业科学,2021,49(9):132-137.
[27]于金平,俞珊,梁有旺,等. NaCl胁迫对美国白蜡幼苗部分生理指标的影响[J]. 植物资源与环境学报,2014,23(1):110-112.
[28]崔庆梅,吴利荣,陈斐,等. 高温胁迫对黄瓜幼苗生理生化及光合作用的影响[J]. 延安大学学报(自然科学版),2021,40(1):23-26.
[29]刘若溪,曹歌,王琪,等. 外源褪黑素对高温胁迫下西瓜幼苗生理特性的影响[J]. 天津农业科学,2023,29(6):7-12.
[30]ALMESELMANI M, DESHMUKH P S, SAIRAM R K, et al. Protective role of antioxidant enzymes under high temperature stress [J]. Plant Science,2006,17(3):382-388.
[31]TSIKAS D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples:analytical and biological challenges [J]. Analytical Biochemistry,2017,524(1):13-30.
[32]WU Z, LIU S, ZHAO J, et al. Comparative responses to silicon and selenium in relation to antioxidant enzyme system and the glutathione-ascorbate cycle in flowering Chinese cabbage(Brassica campestris L. ssp. chinensis var. utilis) under cadmium stress [J]. Environmental & Experimental Botany,2017,133:1-11.
[33]段佳豪,杨锍琰,刘星科,等. 几种植物生长调节剂对棉花耐高温性能的调控效应[J]. 棉花学报,2022,34(6):494-507.
[34]庞强强,周曼,孙晓东,等. 菜心耐热性评价及酶促抗氧化系统对高温胁迫的响应[J]. 浙江农业学报,2020,32(1):72-79.
[35]MANGELSEN E, KILIAN J, BERENDZEN K W, et al. Phylogenetic and comparative gene expression analysis of barley ( Hordeum vulgare ) WRKY transcription factor family reveals putatively retained functions between monocots and dicots [J]. BMC Genomics,2008,9(1):1-17.
[36]WU H L, NI Z F, YAO Y Y, et al. Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem)[J]. Progress in Natural Science: Materials International,2007,18(6):697-705.
[37]LI W X, PANG S Y, LU Z G, et al. Function and mechanism of WRKY transcription factors in abiotic stress responses of plants [J]. Plants,2020,9(11):1515-1530.

相似文献/References:

[1]吕敏,苏建坤,白和盛,等.桃蚜取食和机械损伤对番茄和辣椒 PAL、LOX 和 PPO 活性的诱导作用[J].江苏农业学报,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
 Lv?Min,SU Jian-kun,BAI He-sheng,et al.The activities of PAL, LOX and PPO in tomato and pepper plants induced by aphid herbivory and mechanical damage[J].,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
[2]吴淑华,赵文浩,李廷芳,等.南京辣椒上一种斑驳类型病毒病的分子鉴定[J].江苏农业学报,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
 WU Shu-hua,ZHAO Wen-hao,LI Ting-fang,et al.Molecular identification of a virus causing mottle symptoms in pepper leaves in Nanjing[J].,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
[3]郭广君,孙茜,刘金兵,等.基于辣椒基因组重测序的InDel标记开发及应用[J].江苏农业学报,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
 GUO Guang-jun,SUN Qian,LIU Jin-bing,et al.Development and application of pepper InDel markers based on genome re-sequencing[J].,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
[4]李廷芳,吴淑华,赵文浩,等.青海海东设施辣椒轻斑驳病毒的分子检测[J].江苏农业学报,2017,(04):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
 LI Ting-fang,WU Shu-hua,ZHAO Wen-hao,et al.Molecular detection of mild mottle virus isolated from pepper in Haidong, Qinghai province[J].,2017,(06):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
[5]刘潮,韩利红,宋培兵,等.辣椒类甜蛋白基因家族鉴定及表达分析[J].江苏农业学报,2018,(01):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
 LIU Chao,HAN Li-hong,SONG Pei-bing,et al.Identification and expression analysis of thaumatin-like protein gene in pepper[J].,2018,(06):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
[6]王运儒,秦玉燕,杨秀娟,等.40%氯虫·噻虫嗪水分散粒剂在辣椒及土壤中的残留消解动态[J].江苏农业学报,2018,(01):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
 WANG Yun-ru,QIN Yu-yan,YANG Xiu-juan,et al.Dissipation of chlorantraniliprole and thiamethoxam in pepper and soil after field application in the form of 40% water dispersible granules[J].,2018,(06):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
[7]潘宝贵,钱恒彦,戈伟,等.辣椒应答冷信号转导机制研究进展[J].江苏农业学报,2019,(03):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
 PAN Bao-gui,QIAN Heng-yan,GE Wei,et al.Research progress of cold signal transduction mechanisms in pepper[J].,2019,(06):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
[8]高晶霞,吴雪梅,牛勇琴,等.辣根素水乳剂对连作辣椒生长及土壤酶活性的影响[J].江苏农业学报,2021,(01):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
 GAO Jing-xia,WU Xue-mei,NIU Yong-qin,et al.Effect of athomin water emulsion on growth and soil enzyme activities of continuous cropping pepper[J].,2021,(06):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
[9]郭广君,朱雪梅,潘宝贵,等.利用InDel分子标记辅助选育辣椒抗黄瓜花叶病毒病种质[J].江苏农业学报,2021,(05):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
 GUO Guang-jun,ZHU Xue-mei,PAN Bao-gui,et al.Innovation of pepper germplasm resource with resistance to cucumber mosaic virus by InDel molecular marker assisted selection[J].,2021,(06):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
[10]刘雪梅,王悦,李玮琦,等.辣椒miR169及其靶基因的生物信息学分析[J].江苏农业学报,2021,(06):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
 LIU Xue-mei,WANG Yue,LI Wei-qi,et al.Bioinformatics analysis of miR169 and its target genes in pepper[J].,2021,(06):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]

备注/Memo

备注/Memo:
收稿日期:2023-08-15基金项目:江苏省农业科技自主创新基金项目[CX(23)1033];河北省自然科学基金项目(C2019402344)作者简介:李萌楠(1998-),女,河北枣强人,硕士研究生,研究方向为蔬菜病害绿色防控。(Tel)18260079536;(E-mail)a18260079536@163.com通讯作者:乐秀虎,(E-mail)lexiuhu@163.com;周冬梅,(E-mail)dongmeizhou@jaas.ac.cn
更新日期/Last Update: 2024-07-15