[1]李浩龙,周蓉,蒋芳玲,等.醋栗番茄LA2093渐渗系群体苗期耐盐性评价[J].江苏农业学报,2022,38(06):1620-1626.[doi:doi:10.3969/j.issn.1000-4440.2022.06.021]
 LI Hao-long,ZHOU Rong,JJANG Fang-ling,et al.Evaluation of salt tolerance of introgression line population of Solanum pimpinellifolium LA2093 at seedling stage[J].,2022,38(06):1620-1626.[doi:doi:10.3969/j.issn.1000-4440.2022.06.021]
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醋栗番茄LA2093渐渗系群体苗期耐盐性评价()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年06期
页码:
1620-1626
栏目:
园艺
出版日期:
2022-12-31

文章信息/Info

Title:
Evaluation of salt tolerance of introgression line population of Solanum pimpinellifolium LA2093 at seedling stage
作者:
李浩龙周蓉蒋芳玲文军琴刘帅吴震
(南京农业大学园艺学院/农业农村部华东地区园艺作物生物学与种质创新重点实验室,江苏南京210095)
Author(s):
LI Hao-longZHOU RongJJANG Fang-lingWEN Jun-qinLIU ShuaiWU Zhen
(College of Horticulture, Nanjing Agricultural University/Key Laboratory of Biology and Germplasm Innovation of Horticultural Crops in East China, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China)
关键词:
番茄苗期耐盐性渐渗系综合评价
Keywords:
tomatoseedling stagesalt toleranceintrogression linecomprehensive evaluation
分类号:
S641.2
DOI:
doi:10.3969/j.issn.1000-4440.2022.06.021
文献标志码:
A
摘要:
以栽培番茄(Solanum lycopersicum) Jina 为母本,醋栗番茄(Solanum pimpinellifolium)LA2093 为父本,构建渐渗系(introgression line,IL)BC3F3代群体。以该群体的19个渐渗系株系及亲本为试验材料,调查盐胁迫(200 mmol/L NaCl)处理下番茄苗期基于7个指标(株高、茎粗、整株鲜质量、整株干质量、叶绿素相对含量、丙二醛含量和过氧化氢含量)的耐盐性系数。通过对各指标对应的耐盐性系数进行相关性分析、主成分分析和隶属函数分析,综合评价各番茄材料的耐盐性。对各番茄材料的耐盐性综合评价值(D)进行聚类分析,筛选出3个耐盐株系(IL-6、IL-9和IL-12);通过逐步回归分析建立番茄苗期耐盐性预测方程:D=1.155α2+0.576α3+0.525α-16+0.341α-17-1.582,并筛选出茎粗、整株鲜质量、丙二醛含量和过氧化氢含量作为番茄苗期耐盐性评价指标。通过测定番茄幼苗的茎粗、整株鲜质量、丙二醛含量和过氧化氢含量,进而分析相应的耐盐系数,可以对番茄耐盐性进行预测。
Abstract:
BC3F3 population of introgression line (IL) was constructed with cultivar tomato Jina (Solanum lycopersicum) as female parent and wild tomato LA2093 (Solanum pimpinellifolium) as male parent. By using 19 individual plants from BC3F3 ILs and their parents as experimental materials, salt tolerance coefficients derived from seven indexes (plant height, stem diameter, plant fresh weight, plant dry weight, relative chlorophyll content, malondialdehyde content and hydrogen peroxide content) of tomato seedling under salt stress (200 mmol/L NaCl) were investigated. The salt tolerance coefficients of each index were used to do correlation analysis, principal component analysis and affiliation function analysis to comprehensively evaluate the salt tolerance of each tomato material. Cluster analysis was performed on the comprehensive salt tolerance evaluation value (D) of each material, and three salt-tolerant strains (IL-6, IL-9 and IL-12) were selected. The salt tolerance prediction equation was established by stepwise regression analysis: D=1.155α2+0.576α3+0.525α-16+0.341α-17-1.582, and stem diameter, plant fresh weight, malondialdehyde content and hydrogen peroxide content were selected as the evaluation indexes of salt tolerance for tomato seedlings. Salt tolerance of tomato seedling can be predicted by measuring stem diameter, plant fresh weight, malondialdehyde content and hydrogen peroxide content and analyzing their related salt tolerance coefficients.

参考文献/References:

[1]郏艳红, 刘仲齐, 金凤媚, 等. 番茄耐盐性研究进展[J]. 天津农业科学, 2006(2): 20-23.
[2]FOOLAD M R, LIN G Y. Absence of a genetic relationship between salt tolerance during seed germination and vegetative growth in tomato[J]. Plant Breeding, 1997, 116(4): 363-367.
[3]黄秋凤. 番茄渐渗系IL9-1耐旱基因的定位和候选[D]. 武汉:华中农业大学, 2016.
[4]ASINS M J,VILLALTA I,ALY M M,et al. Two closely linked tomato HKT coding genes are positional candidates for the major tomato QTL involved in Na+/K+ homeostasis[J]. Plant, Cell & Environment, 2013, 36(6): 1171-1191.
[5]FOOLAD M R, LIN G Y. Genetic potential for salt tolerance during germination in Lycopersicon species[J]. Hort Science, 1997, 32(2): 296-300.
[6]FOOLAD M R. Genetic basis of physiological traits related to salt tolerance in tomato, Lycopersicon esculentum Mill[J]. Plant Breeding, 1997, 116(1): 53-58.
[7]TAL M, SHANNON M C. Salt Tolerance in the wild relatives of the cultivated tomato: responses of Lycopersicon esculentum, L. cheesmanii, L. peruvianum, Solanum pennellii and F1 hybrids to high salinity[J]. Functional Plant Biology, 1983, 10(1): 109-117.
[8]周龙溪. 野生种番茄Solanum pennellii苗期耐盐主效QTL的精细定位[D]. 北京:中国农业科学院, 2013.
[9]余庆辉,刘磊,王柏柯,等. 利用Solanum pennellii LA716 渐渗系群体对番茄苗期耐盐 QTLs 进行定位及 QTL 效应的初步分析[J]. 中国农业科学, 2010, 43(4): 761-768.
[10]刘希艳. 醋栗番茄(Solanum pimpinellifolium)RIL群体苗期耐盐耐旱QTL定位[D]. 北京:中国农业科学院, 2016.
[11]潘颖,王孝宣,杜永臣,等. 利用高代回交群体定位野生醋栗番茄发芽期耐盐QTL[J]. 园艺学报, 2010, 37(1): 39-46.
[12]LI J M, LIU L,BAI Y L,et al. Seedling salt tolerance in tomato[J]. Euphytica, 2011, 178(3): 403-414.
[13]CHOUDHARY A, KAUR N, SHARMA A, et al. Evaluation and screening of elite wheat germplasm for salinity stress at the seedling phase[J]. Physiol Plant, 2021, 173(4): 2207-2215.
[14]刘彤彤,李宁,魏良迪,等. 山西省主推小麦品种芽期及苗期耐盐性的综合评价[J]. 中国农业大学学报, 2022, 27(2): 22-33.
[15]戴海芳,武辉,阿曼古丽·买买提阿力,等. 不同基因型棉花苗期耐盐性分析及其鉴定指标筛选[J]. 中国农业科学, 2014, 47(7): 1290-1300.
[16]杨涛,李生梅,黄雅婕,等. 海岛棉资源自然复合盐胁迫综合评价[J]. 核农学报, 2021, 35(7): 1507-1521.
[17]岑本建. 外源褪黑素缓解番茄盐胁迫的机理[D]. 南京:南京农业大学, 2017.
[18]李合生. 植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000.
[19]张则宇,李雪,王焱,等. 59份苜蓿种质材料苗期耐盐性评价及耐盐指标筛选[J]. 草地学报, 2020, 28(1): 112-121.
[20]张瑞,王洋. SHAHID H,等. 水培条件下水稻全生育期耐盐筛选鉴定[J]. 植物遗传资源学报, 2021, 22(6): 1567-1581.
[21]田小霞,毛培春,郑明利,等. 白花草木樨种质苗期耐盐指标筛选及耐盐性综合评价[J]. 中国草地学报, 2019, 41(6):7-14.
[22]刘彤彤,李宁,魏良迪,等. 山西省主推小麦品种芽期及苗期耐盐性的综合评价[J]. 中国农业大学学报, 2022, 27(2): 22-33.
[23]张自强,白晨,张惠忠,等. 甜菜种质资源苗期耐盐性评价及筛选[J]. 中国农学通报, 2020, 36(13): 23-28.
[24]陈新,张宗文,吴斌. 裸燕麦萌发期耐盐性综合评价与耐盐种质筛选[J]. 中国农业科学, 2014, 47(10): 2038-2046.
[25]高三基,罗俊,陈如凯,等. 甘蔗品种抗旱性光合生理指标及其综合评价[J]. 作物学报, 2002,28(1):94-98.
[26]陈建林,吴雪霞,朱为民. NaCl胁迫下不同番茄品种幼苗耐盐性研究[J]. 上海农业学报, 2008(3):80-83.
[27]杨凤军,高凤,韩昱,等. 不同基因型番茄幼苗期耐盐性分析[J]. 黑龙江八一农垦大学学报, 2018,30(4):12-17.
[28]许立志,庞胜群,刁明,等. 隶属函数法评价不同加工番茄品种耐盐性[J].新疆农业科学,2017,54(5):833-842.
[29]董志刚,程智慧. 番茄品种资源芽苗期和幼苗期的耐盐性及耐盐指标评价[J].生态学报,2009,29(3):1348-1355.
[30]SU X, WANG B, GENG X, et al. A high-continuity and annotated tomato reference genome[J]. BMC Genomics, 2021, 22(1):1-12.
[31]史建磊,熊自立,苏世闻,等. 栽培番茄与野生番茄NBS-LRR类抗病基因家族的全基因组鉴定及表达分析[J].南方农业学报,2021,52(5):1158-1166.

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备注/Memo

备注/Memo:
收稿日期:2022-03-01基金项目:国家自然科学基金联合基金项目(U1903106);中央高校基本科研业务费项目(KYZZ2021004);江苏高校优势学科建设工程项目(PAPD)作者简介:李浩龙(1996-),男,山东潍坊人,硕士研究生,主要从事番茄种植资源创新研究。(E-mail)2019104069@stu.njau.edu.cn通讯作者:吴震,(E-mail)wzh@njau.edu.cn
更新日期/Last Update: 2023-01-13