[1]徐港明,符杏,丰华,等.多胺对大豆下胚轴不定根中二胺氧化酶基因表达和酶活性的影响[J].江苏农业学报,2016,(06):1237-1243.[doi:doi:10.3969/j.issn.1000-4440.2016.06.007]
　XU Gang-ming,FU Xing,FENG Hua,et al.Changes of diamine oxidase gene expression and enzyme activity during regeneration of soybean hypocotyl adventitious roots promoted by exogenous polyamines[J].,2016,(06):1237-1243.[doi:doi:10.3969/j.issn.1000-4440.2016.06.007]
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多胺对大豆下胚轴不定根中二胺氧化酶基因表达和酶活性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年06期
页码:
1237-1243
栏目:
遗传育种·生理生化
出版日期:
2017-02-07

文章信息/Info

Title:
Changes of diamine oxidase gene expression and enzyme activity during regeneration of soybean hypocotyl adventitious roots promoted by exogenous polyamines
作者:
徐港明; 符杏; 丰华; 苏国兴
（苏州大学医学部基础医学与生物科学学院，江苏苏州215123）
Author(s):
XU Gang-ming; FU Xing; FENG Hua; SU Guo-xing
（School of Basic and Biological Science，Soochow University，Suzhou 215123, China）
关键词:
大豆下胚轴; 不定根; 腐胺; 亚精胺; 精胺; 二胺氧化酶
Keywords:
soybean hypocotyl; adventitious root; putrescine; spermidine; spermine; diamine oxidase
分类号:
S565.1
DOI:
doi:10.3969/j.issn.1000-4440.2016.06.007
文献标志码:
A
摘要:
为探究不定根再生过程中多胺对多胺氧化酶的调控机制，用外源多胺及多胺氧化酶抑制剂处理大豆幼苗下胚轴，检测大豆下胚轴不定根再生情况以及二胺氧化酶基因（CuAO）表达和酶活性的变化。结果显示，低浓度的腐胺、亚精胺和精胺都显著促进大豆下胚轴不定根的生长，最适浓度分别为1.00 mmol/L、0.25 mmol/L及0.05 mmol/L，高浓度多胺则对不定根的生长表现出一定的抑制作用。3种外源多胺最适浓度处理后CuAO基因表达明显上调，显著高于对照组，且一直维持较高的水平，CuAO酶活性显著高于对照组。在3种多胺处理中，腐胺处理的效果最好。CuAO专性抑制剂氨基胍可显著抑制大豆下胚轴不定根的生长，这可能与CuAO活性受到氨基胍抑制有关。表明，CuAO基因表达受外源多胺的诱导，多胺促进大豆下胚轴不定根的生长可能与其诱导CuAO基因表达以及促进酶的活性有关。
Abstract:
Soybean [Glycine max(L. ) Merr.] seedling hypocotyls were treated with exogenous polyamine and aminoguanidine to explore the regulating mechanism of polyamine on polyamine oxidase in adventitious root regeneration. The number of adventitious roots, the changes of diamine oxidase gene expression and the enzyme activity were measured. Low concentrations of putrescine (Put), spermidine (Spd) and spermine (Spm) significantly promoted the growth of soybean hypocotyl adventitious roots, and the optimum concentrations were 1.00 mol/L、0.25 mmol/L and 0.05 mmol/L, respectively. On the contrary, polyamines at high concentrations showed inhibitory effects. The gene expression level of CuAO was up-regulated by polyamines and maintained a high level afterwards. Correspondingly, the CuAO activity was gradually increased by exogenous polyamines during the regeneration of adventitious roots. Put showed the best effect. In addition, the role of aminoguanidine (AG, a specific inhibitor of CuAO) was detected. It could significantly inhibit the growth of soybean adventitious root, which might be related to the inhibition of CuAO activity by AG. These results imply that the expression of CuAO gene can be induced by exogenous polyamines, the induction of CuAO gene expression and the improvement of the enzyme activity might have contributed to the promotion of soybean hypocotyl adventitious root growth.

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备注/Memo

备注/Memo:
收稿日期：2016-02-19 基金项目：国家自然科学基金项目（31171467） 作者简介：徐港明（1990-），男，湖南岳阳人，硕士研究生，主要从事植物生长发育研究。（E-mail）164807616@qq.com 通讯作者：苏国兴，（E-mail）suguoxing@suda.edu.cn
更新日期/Last Update: 2017-02-07