[1]张高伟,王宣朋,吴春,等.嗜水气单胞菌感染后中华绒螯蟹肝胰腺的转录组分析[J].江苏农业学报,2025,(10):2018-2026.[doi:doi:10.3969/j.issn.1000-4440.2025.10.015]
　ZHANG Gaowei,WANG Xuanpeng,WU Chun,et al.Transcriptomic analysis of hepatopancreas of Eriocheir sinensis infected with Aeromonas hydrophila[J].,2025,(10):2018-2026.[doi:doi:10.3969/j.issn.1000-4440.2025.10.015]
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嗜水气单胞菌感染后中华绒螯蟹肝胰腺的转录组分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
2018-2026
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-10-31

文章信息/Info

Title:
Transcriptomic analysis of hepatopancreas of Eriocheir sinensis infected with Aeromonas hydrophila
作者:
张高伟; 王宣朋; 吴春; 王信海; 高丹丹
(江苏省农业科学院宿迁农业科学研究所,江苏宿迁223800)
Author(s):
ZHANG Gaowei; WANG Xuanpeng; WU Chun; WANG Xinhai; GAO Dandan
(Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian 223800, China)
关键词:
中华绒螯蟹; 嗜水气单胞菌; 肝胰腺组织; 转录组; 差异表达基因
Keywords:
Eriocheir sinensis; Aeromonas hydrophila; hepatopancreatic tissues; transcriptome; differentially expressed genes
分类号:
S917.4
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.015
文献标志码:
A
摘要:
本研究旨在解析嗜水气单胞菌(Aeromonas hydrophila)感染对中华绒螯蟹(Eriocheir sinensis)基因表达调控的影响机制,通过RNA-Seq比较雌雄个体在病原侵染24 h后肝胰腺组织的基因差异表达谱,初步分析雄性中华绒螯蟹和雌性中华绒螯蟹是否存在免疫二态性。结果显示,雌性中华绒螯蟹感染组和对照组共富集到3 447个差异表达基因,包括1 424个上调表达基因和2 023个下调表达基因；雄性中华绒螯蟹感染组和对照组共富集到3 035个差异表达基因,包括1 462个上调表达基因和1 573个下调表达基因。雌蟹和雄蟹富集到免疫相关的差异表达基因主要有泛素蛋白连接酶基因、Toll样受体基因、C型凝集素基因、热休克蛋白基因、溶菌酶基因等。GO富集分析结果显示,大量差异表达基因被富集在免疫相关的生物过程。KEGG富集分析结果表明,差异表达基因显著富集在118个通路,其中免疫相关的代谢通路主要有溶酶体、细胞外基质（ECM)受体相互作用、过氧化物酶体和吞噬体等。此外,挑选转录组数据中的9个免疫相关基因进行qRT-PCR验证,验证结果与RNA-Seq结果一致。本研究初步探究了中华绒螯蟹肝胰腺响应嗜水气单胞菌侵染的分子机制。
Abstract:
To investigate the regulatory mechanism of gene expression in Eriocheir sinensis (Chinese mitten crab) upon infection with Aeromonas hydrophila, we compared the differential gene expression profiles of hepatopancreas tissues between male and female individuals at 24 hours post-pathogen infection through RNA-Seq, and preliminarily analyzed whether there is immune dimorphism between male and female Eriocheir sinensis. The results showed that a total of 3 447 differentially expressed genes (DEGs) were identified between the infected group and the control group in female crabs, including 1 424 up-regulated genes and 2 023 down-regulated genes. In male crabs, 3 035 DEGs were enriched between the infected group and the control group, with 1 462 up-regulated genes and 1 573 down-regulated genes. The immune-related DEGs enriched in both female and male crabs mainly included ubiquitin-protein ligase genes, Toll-like receptor genes, C-type lectin genes, heat shock protein genes, and lysozyme genes. GO enrichment analysis revealed that a large number of DEGs were enriched in immune-related biological processes. KEGG enrichment analysis indicated that DEGs were significantly enriched in 118 pathways, and the immune-related metabolic pathways mainly included lysosome, extracellular matrix (ECM)-receptor interaction, peroxidase, and phagosome. In addition, nine immune-related genes from the transcriptome data were selected for qRT-PCR validation. The results were consistent with those from RNA-Seq. This study preliminarily explored the molecular mechanism underlying the hepatopancreas of Eriocheir sinensis in response to Aeromonas hydrophila infection.

参考文献/References:

[1]赵大显,陈立侨. 中华绒螯蟹免疫相关基因的研究进展[J]. 水产科学,2010,29(4):243-248.
[2]吴淑勤, 王亚军. 我国水产养殖病害控制技术现状与发展趋势[J]. 中国水产,2010(8):9-10.
[3]王晶晶,方苹,陈静,等. 2021年江苏省河蟹养殖病情监测分析[J]. 水产养殖,2022,43(12):69-74.
[4]宫子慧,孙修云,王守军,等. 泗洪县河蟹养殖细菌性疾病病原菌分离,鉴定和药敏试验[J]. 科学养鱼,2019(2):45-47.
[5]郑世雄. 池塘养殖中华绒螯蟹细菌性疾病的病原鉴定及流行病学调查[D]. 合肥:安徽农业大学,2013.
[6]ZHANG X N, SHEN G Q, GUO Y N, et al. Genome-wide identification and analysis of the MAPKK gene family in Chinese mitten crab (Eriocheir sinensis) and its response to bacterial challenge[J]. Fish & Shellfish Immunology,2023,143:109132.
[7]赵庆. 魁蚶血红蛋白基因的克隆及功能分析[D]. 上海:上海海洋大学,2018.
[8]沈锦玉,尹文林,钱冬,等. 中华绒螯蟹“腹水病”及“抖抖病”并发病病原的研究[J]. 中国水产科学,2000,7(3):89-92.
[9]姜艳丽,李丽丽. 嗜水气单胞菌的研究进展[J]. 黑龙江农业科学,2015(2):142-146.
[10]张瑶. 中华绒螯蟹肝胰腺病症的病理分析[D]. 上海:上海海洋大学,2013.
[11]ZHANG Y, LI Z Y, KHOLODKEVICH S, et al. Cadmium-induced oxidative stress,histopathology,and transcriptome changes in the hepatopancreas of freshwater crayfish (Procambarus clarkii)[J]. Science of the Total Environment,2019,666:944-955.
[12]REN X Y, PAN L Q, WANG L. The detoxification process,bioaccumulation and damage effect in juvenile white shrimp Litopenaeus vannamei exposed to chrysene[J]. Ecotoxicology and Environmental Safety,2015,114:44-51.
[13]CHENG C H, MA H L, DENG Y Q, et al. Effects of Vibrio parahaemolyticus infection on physiological response,histopathology and transcriptome changes in the mud crab (Scylla paramamosain)[J]. Fish & Shellfish Immunology,2020,106:197-204.
[14]XUAN F J, FU L L, ZHAO X Y, et al. Molecular dominance investigation of large-sized Chinesemitten crab(Eriocheir sinensis)parents based on themale accessory gland transcriptome[J]. Aquaculture Research,2021,52(8):3498-3507.
[15]REN X Y, LIU P, LI J. Comparative transcriptomic analysis of Marsupenaeus japonicus hepatopancreas in response to Vibrio parahaemolyticus and white spot syndrome virus[J]. Fish & Shellfish Immunology,2019,87:755-764.
[16]FANG F, YUAN Y, JIN M, et al. Hepatopancreas transcriptome analysis reveals the molecular responses to different dietary n-3 PUFA lipid sources in the swimming crab Portunus trituberculatus[J]. Aquaculture,2021,543:737016.
[17]刘文静,杨丽诗,李晓兰,等. 斑节对虾Relish基因的克隆及免疫刺激对其mRNA表达水平的影响[J]. 中国水产科学,2013,20(1):50-60.
[18]LI F H, XIANG J H. Signaling pathways regulating innate immune responses in shrimp[J]. Fish & Shellfish Immunology,2013,34(4):973-980.
[19]HENNESSY F, NICOLL W S, ZIMMERMANN R, et al. Not all J domains are created equal:implications for the specificity of Hsp40-Hsp70 interactions[J]. Protein Science,2005,14(7):1697-1709.
[20]谢建辉,顾建新. C型凝集素[J]. 生命科学,2011,23(6):555-562.
[21]刘鑫潮. 罗非鱼肝凝集素基因的鉴定及其功能研究[D]. 湛江:广东海洋大学, 2021.
[22]ZHANG X W, WANG Y, WANG X W, et al. A C-type lectin with an immunoglobulin-like domain promotes phagocytosis of hemocytes in crayfish Procambarus clarkii[J]. Scientific Reports,2016,6:29924.
[23]YANG Q Y, WANG P, WANG S, et al. The hepatic lectin of zebrafish binds a wide range of bacteria and participates in immune defense[J]. Fish & Shellfish Immunology,2018,82:267-278.
[24]DUAN Y F, ZHANG J S, DONG H B, et al. Oxidative stress response of the black tiger shrimp Penaeus monodon to Vibrio parahaemolyticus challenge[J]. Fish & Shellfish Immunology,2015,46(2):354-365.
[25]GUO H, LI K X, WANG W, et al. Effects of copper on hemocyte apoptosis,ROS production,and gene expression in white shrimp litopenaeus vannamei[J]. Biological Trace Element Research,2017,179(2):318-326.
[26]HONG Y H, YIN H M, HUANG Y, et al. Immune response to abamectin-induced oxidative stress in Chinese mitten crab,Eriocheir sinensis[J]. Ecotoxicology and Environmental Safety,2020,188:109889.
[27]王超,张龙岗,吴蒙蒙,等. 吞噬作用在尼罗罗非鱼抵抗嗜水气单胞菌感染过程中的重要作用[J]. 中国水产科学,2020,27(3):346-354.
[28]ZHAO L J, TU J G, ZHANG Y L, et al. Transcriptomic analysis of the head kidney of Topmouth Culter (Culter alburnus) infected with Flavobacterium columnare with an emphasis on phagosome pathway[J]. Fish & Shellfish Immunology,2016,57:413-418.
[29]于明. 嗜水气单胞菌感染大口黑鲈肝脏组织mRNA与miRNA组学联合分析[D]. 泰安:山东农业大学,2024.
[30]刘凯,谢楠,戴瑜来,等. 基于转录组测序技术研究三角鲂和团头鲂抗嗜水气单胞菌感染基因表达差异[J]. 渔业研究,2022,44(4):301-311.

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备注/Memo

备注/Memo:
收稿日期：2024-11-04基金项目：宿迁市农业科技自主创新项目(SQCX202310)作者简介：张高伟(1993-),男,江苏徐州人,硕士,助理研究员,主要从事甲壳动物免疫研究。(E-mail)656421404@qq.com通讯作者：王信海,(E-mail)hai92913@163.com
更新日期/Last Update: 2025-11-17