[1]卞晓春,王凡,刘陈玮,等.蚕豆NAC转录因子家族鉴定分析及抗叶斑病基因筛选[J].江苏农业学报,2025,(09):1683-1695.[doi:doi:10.3969/j.issn.1000-4440.2025.09.003]
　BIAN Xiaochun,WANG Fan,LIU Chenwei,et al.Identification of NAC transcription factor family in Vicia faba L. and screening of genes resistant to leaf spot[J].,2025,(09):1683-1695.[doi:doi:10.3969/j.issn.1000-4440.2025.09.003]
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蚕豆NAC转录因子家族鉴定分析及抗叶斑病基因筛选()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1683-1695
栏目:
遗传育种·生理生化
出版日期:
2025-09-30

文章信息/Info

Title:
Identification of NAC transcription factor family in Vicia faba L. and screening of genes resistant to leaf spot
作者:
卞晓春; 王凡; 刘陈玮; 徐仁超; 陆红臣; 吴春芳
(江苏沿江地区农业科学研究所/南通市作物春化技术重点实验室,江苏南通226012)
Author(s):
BIAN Xiaochun; WANG Fan; LIU Chenwei; XU Renchao; LU Hongchen; WU Chunfang
(Jiangsu Yanjiang Institute of Agricultural Sciences/Key Laboratory of Crop Vernalization Technology of Nantong City, Nantong 226012, China)
关键词:
蚕豆; NAC转录因子; 链格孢菌; 抗病基因
Keywords:
Vicia faba L.; NAC transcription factor; Alternaria alternata; disease resistance genes
分类号:
S435.23
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.003
文献标志码:
A
摘要:
NAC转录因子在植物生长发育及逆境胁迫响应中具有重要作用。为明确蚕豆(Vicia faba L.)NAC转录因子的功能,本研究利用生物信息学方法从蚕豆基因组数据库中鉴定蚕豆NAC家族基因成员,分析其基因结构、启动子顺式作用元件、染色体分布以及编码蛋白质的理化性质等,结合造成蚕豆叶斑病的病原菌链格孢菌(Alternaria alternata)侵染的蚕豆转录组数据筛选与抗病相关的候选基因。结果表明,蚕豆NAC转录因子基因家族共有77个成员,不均匀分布于6条染色体上,其编码的蛋白质大小介于133 aa至665 aa之间；VfNAC蛋白分为16个亚家族,均含有NAM保守结构域,均定位于细胞核。VfNAC基因启动子上包含光响应元件、激素响应元件、干旱响应元件等多种顺式作用元件。转录组测序结果显示,链格孢菌侵染后蚕豆中有33个VfNAC基因差异表达,筛选出4个差异表达显著的基因作为关键抗病候选基因进一步研究。本研究为蚕豆NAC转录因子后续的生物学功能解析及抗病分子机制研究提供了参考。
Abstract:
The NAC transcription factors play important roles in plant growth and development as well as in responses to abiotic and biotic stresses. To clarify the functions of NAC transcription factors in Vicia faba L., in this study, bioinformatic methods were used to identify the members of the NAC family genes from the Vicia faba genome database. The gene structures, cis-acting elements in promoters, chromosomal distributions, and the physicochemical properties of the proteins encoded by these genes were analyzed. By integrating the transcriptome data of Vicia faba infected by Alternaria alternata (the pathogen causing leaf spot in Vicia faba), candidate genes related to disease resistance were screened. The results showed that there were 77 members in the NAC transcription factor gene family of Vicia faba, which were unevenly distributed on six chromosomes. The encoded proteins ranged in size from 133 aa to 665 aa. The VfNAC proteins were divided into 16 subfamilies, all of which contained the conserved NAM domain and were localized to the nucleus. The promoters of VfNAC genes contained various cis-acting elements such as light-responsive elements, hormone-responsive elements, and drought-responsive elements. The transcriptome sequencing results showed that 33 VfNAC genes were differentially expressed in Vicia faba infected by Alternaria alternata, and four genes exhibiting significant differential expression were selected as key candidate disease-resistance genes for further study. This study provides a reference for the subsequent analysis of the biological functions of NAC transcription factors in Vicia faba and the research on the molecular mechanisms of disease resistance.

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备注/Memo

备注/Memo:
收稿日期：2024-10-18基金项目：江苏省种业揭榜挂帅项目[JBGS(2021)058]；江苏现代农业产业技术体系建设专项[JATS(2023)158]；江苏省重点研发计划(现代农业)项目(BE2021389)；江苏沿江地区农业科学研究所青年科技基金项目[YJ(2022)009]作者简介：卞晓春(1987-),男,江苏盐城人,硕士,副研究员,主要从事蚕豆抗病遗传育种。(E-mail)oneise@163.com通讯作者：吴春芳,(E-mail)jsyias@163.com
更新日期/Last Update: 2025-10-27