[1]田孟凡,戴威威,周佳慧,等.大蒜内生贝莱斯芽孢杆菌促进蒜氨酸积累[J].江苏农业学报,2024,(12):2283-2291.[doi:doi:10.3969/j.issn.1000-4440.2024.12.011]
　TIAN Mengfan,DAI Weiwei,ZHOU Jiahui,et al.Promotion of alliin accumulation by endogenous Bacillus velezensis in garlic[J].,2024,(12):2283-2291.[doi:doi:10.3969/j.issn.1000-4440.2024.12.011]
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大蒜内生贝莱斯芽孢杆菌促进蒜氨酸积累()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
2283-2291
栏目:
耕作栽培·资源环境
出版日期:
2024-12-30

文章信息/Info

Title:
Promotion of alliin accumulation by endogenous Bacillus velezensis in garlic
作者:
田孟凡1; 2; 戴威威1; 2; 周佳慧1; 薛妍1; 冯昭中1; 王军娟1; 2
(1.江苏师范大学生命科学学院,江苏徐州221000；2.江苏师范大学药用植物生物技术国家重点实验室培育点,江苏徐州221000)
Author(s):
TIAN Mengfan1; 2; DAI Weiwei1; 2; ZHOU Jiahui1; XUE Yan1; FENG Zhaozhong1; WANG Junjuan1; 2
(1.School of Life Sciences, Jiangsu Normal University, Xuzhou 221000, China;2.State Key Laboratory of Medicinal Plant Biotechnology, Jiangsu Normal University, Xuzhou 221000, China)
关键词:
大蒜; 大蒜素; 蒜氨酸; 蒜氨酸裂解酶; 内生菌; 贝莱斯芽孢杆菌
Keywords:
garlic; allicin; alliin; alliinase; endophytic bacteria; Bacillus velezensis
分类号:
S633.4;S182
DOI:
doi:10.3969/j.issn.1000-4440.2024.12.011
文献标志码:
A
摘要:
内生菌与植物之间存在着互利共生关系,它可以促进宿主植物中活性物质的积累。为探究大蒜内生菌能否促进大蒜活性物质蒜氨酸的积累,以贝莱斯芽孢杆菌(Bacillus velezensis)KLBMP108和蜡状芽孢杆菌(Bacillus cereus)KLBMP81为供试菌株,测定其对3个不同地区不同品种大蒜叶片中蒜氨酸含量的影响。结果表明,与对照相比，处理后第6 d, 菌株KLBMP108能使其宿主改良大蒜的蒜氨酸含量提高至1.68 mg/mL,蒜氨酸裂解酶表达量显著提高；而对非宿主品种河南紫蒜以及云南苔蒜的蒜氨酸含量没有显著影响。分离自红豆杉的菌株KLBMP81处理的3种大蒜蒜氨酸含量均较低。本研究证实菌株KLBMP108能够提高其宿主改良大蒜叶片中蒜氨酸含量,是大蒜品种改良的宝贵微生物资源。
Abstract:
A mutually beneficial symbiotic relationship happens between endophytic bacteria and plants, which can promote the accumulation of active substances in the host plant. To investigate whether endophytic bacteria of garlic promote the accumulation of alliin, an active substance in garlic, Bacillus velezensis KLBMP108 and Bacillus cereus KLBMP81 were used as the test strains to determine their effects on the alliin content in leaves of three different varieties of garlic in different regions. The results showed that compared with the control, on the 6th day after treatment, the strain KLBMP108 could increase the alliin content of the improved garlic to 1.68 mg/mL, and the expression of alliinase was significantly increased. However, there was no significant effect on the alliin content of non-host varieties Henan purple garlic and Yunnan moss garlic. The alliin content of three kinds of garlic treated by strain KLBMP81 isolated from Taxus chinensis was low. In conclusion, strain KLBMP108 can increase the alliin content in its host Gailiang garlic leaves, and is a valuable microbial resource for garlic variety improvement.

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备注/Memo

备注/Memo:
收稿日期:2023-12-06基金项目:徐州市科技计划项目基础研究计划青年科技人才项目(KC22022)；国家自然科学基金青年科学基金项目(31801720)作者简介:田孟凡(2000-),女,四川会理人,硕士研究生,主要从事内生菌与植物互作研究。(E-mail)Tianmengfan0526@163.com通讯作者:王军娟,(E-mail)wjj1139@jsnu.edu.cn
更新日期/Last Update: 2025-01-23