[1]王前菊,王宇,仲伟敏,等.基于UPLC-MS/MS的不同植物生长调节剂处理的猕猴桃果实中代谢物差异分析[J].江苏农业学报,2024,(08):1542-1551.[doi:doi:10.3969/j.issn.1000-4440.2024.08.018]
　WANG Qianju,WANG Yu,ZHONG Weimin,et al.Difference analysis of metabolites in kiwifruit treated with different plant growth regulators based on UPLC-MS/MS[J].,2024,(08):1542-1551.[doi:doi:10.3969/j.issn.1000-4440.2024.08.018]
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基于UPLC-MS/MS的不同植物生长调节剂处理的猕猴桃果实中代谢物差异分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年08期
页码:
1542-1551
栏目:
加工贮藏·质量安全
出版日期:
2024-08-30

文章信息/Info

Title:
Difference analysis of metabolites in kiwifruit treated with different plant growth regulators based on UPLC-MS/MS
作者:
王前菊; 王宇; 仲伟敏; 徐特; 唐冬梅
(贵州省果树科学研究所,贵州贵阳550006)
Author(s):
WANG Qianju; WANG Yu; ZHONG Weimin; XU Te; TANG Dongmei
(Guizhou Institute of Fruit Tree Science,Guiyang 550006, China)
关键词:
猕猴桃; UPLC-MS/MS; 氯吡脲（CPPU）; 赤霉素（GA3）; 代谢物; 品质
Keywords:
kiwifruit; UPLC-MS/MS; forchlorfenuron (CPPU); gibberellin (GA3); metabolites; quality
分类号:
S663.4
DOI:
doi:10.3969/j.issn.1000-4440.2024.08.018
文献标志码:
A
摘要:
本研究以金猕为试验材料,探究氯吡脲（CPPU）和赤霉素（GA3）对果实中次生代谢物的影响。基于超高效液相色谱-串联质谱技术(UPLC-MS/MS)对代谢组进行分析,共检测到代谢物411种,包括氨基酸及其衍生物、糖及醇类、核苷酸及其衍生物等。5 mg/L CPPU处理、10 mg/L CPPU处理果实中水平下调代谢物数量高于水平上调代谢物数量，而50 mg/L GA3处理、10 mg/L CPPU+50 mg/L GA3处理果实中水平上调代谢物数量高于水平下调代谢物数量。与对照相比,5 mg/L CPPU处理、10 mg/L CPPU处理、50 mg/L GA3处理、10 mg/L CPPU+50 mg/L GA3处理果实单果重、横径、侧径显著提高(P<0.05)。50 mg/L GA3处理、10 mg/L CPPU+50 mg/L GA3处理果实单果重显著低于5 mg/L CPPU处理、10 mg/L CPPU处理(P<0.05)。综上所述，10 mg/L CPPU+50 mg/L GA3处理金猕产量和品质相对更佳,本研究结果可为金猕栽培及品质评价提供参考。
Abstract:
Using Jinmi kiwifruit as the test material, the effects of forchlorfenuron (CPPU) and gibberellin (GA3) on secondary metabolites in fruits were studied. A total of 411 metabolites, including amino acids and their derivatives, sugars and alcohols, nucleotides and their derivatives, were detected by ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The number of down-regulated metabolites was higher than that of up-regulated metabolites in fruits treated with 5 mg/L CPPU or 10 mg/L CPPU. The number of up-regulated metabolites was higher than that of down-regulated metabolites in fruits treated with 50 mg/L GA3 or 10 mg/L CPPU + 50 mg/L GA3. Compared with the control, the single fruit weight, transverse diameter and lateral diameter of 5 mg/L CPPU treatment, 10 mg/L CPPU treatment, 50 mg/L GA3 treatment and 10 mg/L CPPU + 50 mg/L GA3 treatment increased significantly (P<0.05). The single fruit weight of 50 mg/L GA3 treatment, 10 mg/L CPPU+50 mg/L GA3 treatment was significantly lower than that of 5 mg/L CPPU treatment and 10 mg/L CPPU treatment (P<0.05). It can be concluded that the treatment of 10 mg/L CPPU+50 mg/L GA3 resulted in better yield and quality of Jinmi kiwifruit. This study result can provide reference for Jinmi kiwifruit cultivation and quality evaluation.

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备注/Memo

备注/Memo:
收稿日期:2023-11-20基金项目:国家柑橘产业体系猕猴桃贵阳综合试验站资助项目(CARS-26-65)；贵州省科研机构创新能力建设专项[黔科合服企(2021)8号]作者简介:王前菊(1997-),女,贵州遵义人，硕士,研究实习员,研究方向为果品贮藏与加工。(E-mail)1453491966@qq.com通讯作者:唐冬梅,(E-mail)89368775@qq.com
更新日期/Last Update: 2024-09-18