[1]罗志斌,欧慧敏,李建中,等.体外发酵法评估呼伦贝尔羊瘤胃对过瘤胃蛋氨酸的生物利用率及过瘤胃蛋氨酸对瘤胃微生物的影响[J].江苏农业学报,2024,(05):874-880.[doi:doi:10.3969/j.issn.1000-4440.2024.05.012]
　LUO Zhibin,OU Huimin,LI Jianzhong,et al.Evaluation for bioavailability of rumen protected methionine and its effects on rumen microorganisms in Hulun Buir sheep by in vitro fermentation[J].,2024,(05):874-880.[doi:doi:10.3969/j.issn.1000-4440.2024.05.012]
点击复制

体外发酵法评估呼伦贝尔羊瘤胃对过瘤胃蛋氨酸的生物利用率及过瘤胃蛋氨酸对瘤胃微生物的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年05期
页码:
874-880
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2024-05-30

文章信息/Info

Title:
Evaluation for bioavailability of rumen protected methionine and its effects on rumen microorganisms in Hulun Buir sheep by in vitro fermentation
作者:
罗志斌1; 2; 欧慧敏1; 2; 李建中2; 谭支良1; 焦金真1
(1.中国科学院亚热带农业生态研究所/亚热带农业生态过程重点实验室/畜禽养殖污染控制与资源化技术国家工程实验室/湖南省动物营养生理与代谢过程重点实验室,湖南长沙410125；2.湖南师范大学生命科学学院/动物肠道功能湖南省重点实验室,湖南长沙410081)
Author(s):
LUO Zhibin1; 2; OU Huimin1; 2; LI Jianzhong2; TAN Zhiliang1; JIAO Jinzhen1
(1.Institute of Subtropical Agriculture， Chinese Academy of Sciences/Key Laboratory of Agro-ecological Processes in Subtropical Region/National Engineering Laboratory for Poultry Breeding Pollution Control and Resource Technology/Hunan Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha 410125, China;2.College of Life Sciences, Hunan Normal University/Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Changsha 410081, China)
关键词:
过瘤胃蛋氨酸; 绵羊; 体外发酵; 16S rDNA; 微生物
Keywords:
rumen protected methionine; sheep; in vitro fermentation; 16S rDNA; microorganism
分类号:
S852.23
DOI:
doi:10.3969/j.issn.1000-4440.2024.05.012
摘要:
为了探究呼伦贝尔羊瘤胃体外发酵体系对过瘤胃蛋氨酸(RPMet,由玉米淀粉和棕榈油包被)的生物利用率及过瘤胃蛋氨酸对瘤胃微生物类群的影响,取4只装有瘘管的雌性呼伦贝尔羊的瘤胃液进行体外发酵试验,在发酵起始阶段与发酵后2 h、4 h、6 h、8 h、12 h、24 h测定发酵体系中的RPMet降解率。每个发酵时间节点设置5个平行处理,在以上7个时间节点取发酵液并进行16S rDNA测序分析。结果表明,在体外发酵体系中,RPMet在发酵后8 h的降解率小于30.0%,说明该产品的包被效果较好。测序结果显示,发酵24 h后的Simpson指数显著低于发酵12 h后的Simpson指数(P<0.05)。主坐标分析(PCoA)能够显示物种的β-多样性,本研究结果显示,各时间点的聚类结果皆存在显著差异(P<0.05)。拟杆菌门、瘤胃球菌属、普雷沃氏菌属菌的相对丰度在发酵后8 h内显著上升,发酵12 h后显著下降(P<0.05)。厚壁菌门、广古菌门、甲烷杆菌属、解琥珀酸菌属、颤螺菌属菌的相对丰度在发酵后12 h内显著下降,之后显著上升(P<0.05)。综上所述,研究中所用的RPMet的包被效果较好,且发酵前期主要是普雷沃氏菌属、瘤胃球菌属微生物降解玉米淀粉包被层,而RPMet的过度降解显著提高了甲烷杆菌属微生物的相对丰度。
Abstract:
The purpose of this study was to investigate the bioavailability of rumen-protected methionine (RPMet, coated with corn starch and palm oil) in the rumen in vitro fermentation system of Hulun Buir sheep and the effects of rumen-protected methionine on rumen microbial groups. The rumen fluids of four female Hulun Buir sheep with fistulas were taken for in vitro fermentation test. The degradation rate of RPMet in the fermentation system was determined at the initial stage of fermentation and 2 h, 4 h, 6 h, 8 h, 12 h and 24 h after fermentation. Five parallel treatments were set up at each fermentation time node, and the fermentation broth was taken at the above seven time nodes for 16S rDNA sequencing analysis. The results showed that the degradation rate of RPMet was less than 30.0% at 8 h after fermentation in the in vitro fermentation system, indicating that the coating effect of the product was good. The sequencing results showed that the Simpson index at 24 h after fermentation was significantly lower than that at 12 h after fermentation (P<0.05). Principal coordinate analysis (PCoA) could show the β-diversity of species. The results of this study showed that there were significant differences in the clustering results at each time point (P<0.05). The relative abundance of Bacteroidetes, Ruminococcus and Prevotella increased significantly within 8 h after fermentation, and decreased significantly after 12 h of fermentation (P<0.05). The relative abundance of Firmicutes, Euryarchaeota, Methanobrevibacter, Succiniclasticum and Oscillibacter decreased significantly within 12 h after fermentation, and then increased significantly (P<0.05). In summary, the coating effect of the RPMet product is good. The Prevotella and Ruminococcus act as main genera to degrade the coating layer of corn starch during the early fermentation period, while excessive degradation of RPMet significantly increase the abundance of Methanobrevibacter.

参考文献/References:

[1]杨志林,李蓓蓓,冯建芳. 过瘤胃氨基酸在反刍动物生产中的应用研究[J]. 饲料博览,2016(8):18-20.
[2]李莉,凌浩,雷坤,等. 过瘤胃蛋氨酸应用研究进展[J]. 中国奶牛,2019(8):16-19.
[3]卢玉飞,张雪元,马婷婷,等. 过瘤胃蛋氨酸在反刍动物中的营养研究进展[J]. 饲料工业,2014,35(17):13-18.
[4]苟红晶,高宪儒. 日粮添加过瘤胃蛋氨酸对肉羊生长性能、养分消化率及血液生化指标的影响[J]. 饲料研究,2021,44(7):21-24.
[5]SILVA G M, CHALK C D, RANCHES J, et al. Effect of rumen-protected methionine supplementation to beef cows during the periconception period on performance of cows,calves,and subsequent offspring[J]. Animal,2021,15(1):100055.
[6]丁大伟,高许雷,滕乐帮,等. 过瘤胃蛋氨酸与过瘤胃赖氨酸不同组合对奶牛瘤胃微生物蛋白产量、产奶性能和氮排泄的影响[J]. 动物营养学报,2019,31(6):2716-2726.
[7]王菲,韩娥,任金朋,等. 低蛋白质水平饲粮添加过瘤胃蛋氨酸对黔北麻羊生长性能、养分消化率、血浆生化和抗氧化指标及瘤胃发酵参数的影响[J]. 动物营养学报,2020,32(3):1262-1271.
[8]ABDELMEGEID M K, ELOLIMY A A, ZHOU Z, et al. Rumen-protected methionine during the peripartal period in dairy cows and its effects on abundance of major species of ruminal bacteria[J]. Journal of Animal Science and Biotechnology,2018,9(2):460-466.
[9]MENKE K, RAAB L, SALEWSK A, et al. The estimaton of the dioestibiiy and metabolizable eneray content of uminant feedinastufts from the gas production when they are incubated with rumen liguor in vitro[J]. The Journal of Agricultural Science,1979,93(1):217-222.
[10]WANG M, WANG R, YANG S, et al. Effects of three methane mitigation agents on parameters of kinetics of total and hydrogen gas production，ruminal fermentation and hydrogen balance using in vitro technique[J]. Animal Science Journal,2016,87(2):224-232.
[11]高新梅,郑猛,牛露,等. 育肥羊饲喂全混合日粮条件下瘤胃固相食糜外流速度及其有效降解率的评定[J]. 中国畜牧兽医,2017,44(2):456-462.
[12]郭玉琴. 蛋氨酸和赖氨酸过瘤胃保护及其效果评价研究[D]. 北京：中国农业科学院,2006.
[13]ROSSI F, MAURIZIO M, FRANCESCO M, et al. Rumen degradation and intestinal digestibility of rumen protected amino acids: comparison between in situ and in vitro data[J]. Animal Feed Science and Technology,2003,108(1/2/3/4):223-229.
[14]吴琼,王思珍,张适,等. 基于16S rRNA高通量测序技术分析安格斯牛瘤胃微生物多样性和功能预测的研究[J].微生物学杂志,2020,40(2):49-56.
[15]李烨青,奚雨萌,曾涵芳,等. 基于16S rRNA测序分析奶牛体外发酵中瘤胃细菌多样性的变化[J]. 动物营养学报,2018，30(10):4059-4070.
[16]SINGH K M, AHIR V B, TRIPATHI A K, et al. Metagenomicanalysis of Surti buffalo (Bubalus bubalis) rumen: a preliminary study[J]. Molecular Biology Reports,2012,39(4):4841-4848.
[17]胡丹丹,郭婷婷,李娜,等. 以16S rDNA高通量测序法研究果寡糖对奶牛瘤胃菌群结构及多样性的影响[J]. 中国畜牧兽医,2018,45(10):2761-2771.
[18]BHATT V D, DANDE S S, PATIL N V, et al. Molecular analysis of the bacterial microbiome in the forestomach fluid from the dromedary camel (Camelus dromedarius)[J]. Molecular Biology Reports,2013,40(4):3363-3371.
[19]HRNCIROVA L, MACHOVA V, TRCKOVA E, et al. Food preservatives induce proteobacteria dysbiosis in human-microbiota associated Nod2-deficient mice[J]. Microorganisms,2019,7(10):383.
[20]SUEN G, STEVENSON D M, BRUCE D C, et al. Complete genome of the cellulolytic ruminal bacterium Ruminococcus albus 7[J]. Journal of Bacteriology,2011,193(19):5574-5575.
[21]RAMAK A, PETERKA M, TAJIMA K, et al. Unravelling the genetic diversity of ruminal bacteria belonging to the CFB phylum[J]. FEMS Microbiology Ecology,2000,33(1):69-79.
[22]WANG Z, YU Y N, LI X Y, et al. Fermented soybean meal replacement in the diet of lactating holstein dairy cows: modulated rumen fermentation and ruminal microflora[J]. Frontiers in Microbiology,2021,12:625857.
[23]LEE G H, KUMAR S, LEE J H, et al. Genome sequence of Oscillibacter ruminantium strain GH1, isolated from rumen of Korean native cattle[J]. Journal of Bacteriology,2012,194(22):6362.
[24]CHRISTINE M E, MANUELA P, JULIAN T, et al. Archaea are interactive components of complex microbiomes[J]. Trends in Microbiology,2018,26(1):70-85.

相似文献/References:

[1]吴阳升,林嘉鹏,汪立芹,等.绵羊小卵泡与中卵泡转录组差异特征分析[J].江苏农业学报,2016,(04):832.[doi:10.3969/j.issn.100-4440.2016.04.019]
　WU Yang-sheng,LIN Jia-peng,WANG Li-qin,et al.Transcriptome profiling of ovine follicles during growth from small to middle antral sizes[J].,2016,(05):832.[doi:10.3969/j.issn.100-4440.2016.04.019]
[2]闫乐艳,GEORGE Mann,施振旦.利用Onapristone研究绵羊子宫内膜组织中孕酮对PGF2α分泌以及COX-2表达的影响[J].江苏农业学报,2017,(03):624.[doi:doi:10.3969/j.issn.1000-4440.2017.03.020]
　YAN Le-yan,GEORGE Mann,SHI Zhen-dan.Regulation of progesterone in PGF2α secretion and COX-2 expression by onapristone in ovine endometrial cells[J].,2017,(05):624.[doi:doi:10.3969/j.issn.1000-4440.2017.03.020]
[3]吴阳升,林嘉鹏,蒋香菊,等.绵羊FSHR基因可变剪接体的克隆、鉴定及表达分析[J].江苏农业学报,2017,(03):630.[doi:doi:10.3969/j.issn.1000-4440.2017.03.021]
　WU Yang-sheng,LIN Jia-peng,JIANG Xiang-ju,et al.Cloning, identification, and expression analysis of alternative splicing isoforms of FSHR in sheep[J].,2017,(05):630.[doi:doi:10.3969/j.issn.1000-4440.2017.03.021]
[4]卢春霞,刘长彬,万鹏程,等.绵羊ovPAG7间接竞争酶联适配体检测方法的建立与应用[J].江苏农业学报,2022,38(03):730.[doi:doi:10.3969/j.issn.1000-4440.2022.03.019]
　LU Chun-xia,LIU Chang-bin,WAN Peng-cheng,et al.Establishment and application of indirect competitive enzyme-linked aptamer assay for ovPAG7 in sheeps[J].,2022,38(05):730.[doi:doi:10.3969/j.issn.1000-4440.2022.03.019]
[5]孟科,赵薇,郭晨浩,等.不同品种绵羊肌内脂肪沉积相关miRNA的筛选与功能预测[J].江苏农业学报,2023,(07):1554.[doi:doi:10.3969/j.issn.1000-4440.2023.07.012]
　MENG Ke,ZHAO Wei,GUO Chen-hao,et al.Screening and functional prediction of miRNA related to intramuscular fat deposition in different sheep breeds[J].,2023,(05):1554.[doi:doi:10.3969/j.issn.1000-4440.2023.07.012]
[6]李述方,王海荣.热应激对绵羊机体氧化损伤及免疫功能的影响[J].江苏农业学报,2023,(07):1606.[doi:doi:10.3969/j.issn.1000-4440.2023.07.017]
　LI Shu-fang,WANG Hai-rong.Effects of heat stress on oxidative damage and immune function in sheep[J].,2023,(05):1606.[doi:doi:10.3969/j.issn.1000-4440.2023.07.017]

备注/Memo

备注/Memo:
收稿日期：2023-06-13基金项目：中国科学院国际伙伴计划项目(161343KYSB20200015)作者简介：罗志斌(1999-),男,湖南娄底人,硕士研究生,从事反刍动物营养研究。(E-mail)1851375407@qq.com通讯作者：焦金真,(E-mail)jjz@isa.ac.cn
更新日期/Last Update: 2024-07-13