[1]纪伟,苏文英,刘晓梅,等.连云港地区野生灵芝生物学特性及基因组特征分析[J].江苏农业学报,2024,(02):223-232.[doi:doi:10.3969/j.issn.1000-4440.2024.02.004]
 JI Wei,SU Wen-ying,LIU Xiao-mei,et al.Analysis of biological characteristics and genomic characteristics of wild Ganoderma lucidum in Lianyungang[J].,2024,(02):223-232.[doi:doi:10.3969/j.issn.1000-4440.2024.02.004]
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连云港地区野生灵芝生物学特性及基因组特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年02期
页码:
223-232
栏目:
遗传育种·生理生化
出版日期:
2024-02-25

文章信息/Info

Title:
Analysis of biological characteristics and genomic characteristics of wild Ganoderma lucidum in Lianyungang
作者:
纪伟12苏文英1刘晓梅1任立凯1胡曙鋆3孙潇潇3陈克龙2
(1.连云港市农业科学院,江苏连云港222000;2.青海师范大学,青海西宁810000;3.连云港市园艺蔬菜指导站,江苏连云港222000)
Author(s):
JI Wei12SU Wen-ying1LIU Xiao-mei1REN Li-kai1HU Shu-yun3SUN Xiao-xiao3CHEN Ke-long2
(1.Lianyungang Academy of Agricultural Sciences, Lianyungang 222000, China;2.Qinghai Normal University, Xining 810000, China;3.Lianyungang Gardening Vegetable Guidance Station, Lianyungang 222000, China)
关键词:
灵芝生物学特性全基因组重测序主成分分析
Keywords:
Ganoderma lucidumbiological characteristicswhole genome resequencingprincipal component analysis
分类号:
S567.3+1
DOI:
doi:10.3969/j.issn.1000-4440.2024.02.004
摘要:
野生灵芝资源在连云港境内有着广泛的分布,本研究使用全基因组重测序技术对具有不同形态特征和生境特点的灵芝基因组进行测序及分析,以期解析连云港地区野生灵芝的生物学特性及基因组特征。结果表明,从不同区域采集的野生灵芝种质资源菌丝具有锁状联合,具备形成子实体的条件,生物学特性存在较大差异;通过主成分分析将12株野生灵芝菌株种质资源聚为3类,其中第Ⅲ类具有更丰富的遗传多样性;比较单核苷酸多态性(SNP)、插入或者缺失(InDel)在12株灵芝菌株基因组中的分布情况发现,第1条染色体中的SNP最多,占比为46.07%,SNP中转换颠换比(Ts/Tv)为2.03,说明变异以转换为主,插入或缺失导致的基因差异在第1条染色体上最多,占总InDel数的44.31%。研究结果说明,连云港地区灵芝具有丰富的遗传多样性,可以用于进一步挖掘更多的功能基因和进行品种选育。
Abstract:
Wild Ganoderma lucidum resources are extensively found in Lianyungang. In this study, we utilized whole-genome resequencing technology to examine and analyze the genomes of Ganoderma lucidum with different morphological and habitat characteristics. The purpose of this study was to unveil the biological and genomic characteristics of wild Ganoderma lucidum in Lianyungang. The results showed that the mycelium of wild Ganoderma lucidum germplasm resources collected from different regions had clamp connection, and had the conditions to form fruiting bodies, and the biological characteristics were quite different. Through principal component analysis, 12 wild Ganoderma lucidum germplasm resources were grouped into three categories, and the third category had more abundant genetic diversity. Analyzing the distribution of single nucleotide polymorphism (SNP) and insertion or deletion (InDel) in the genome of 12 strains of Ganoderma lucidum, it was found that the number of SNP on chromosome 1 was the largest, accounting for 46.07%, and the conversion and inversion ratio (Ts/Tv) in the SNP was 2.03, indicating that the variation was mainly conversion. The genetic difference caused by insertion or deletion was the highest on chromosome 1, accounting for 44.31% of the total InDel number. These results showed that Ganoderma lucidum in Lianyungang had rich genetic diversity, which could be used to further explore more functional genes and breed varieties.

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备注/Memo

备注/Memo:
收稿日期:2023-02-13基金项目:江苏现代农业(蔬菜)产业技术体系[JATS(2022)177];连云港市财政专项(QNJJ2209);连云港市食用菌全产业链建设项目[连农复(2023)14号]作者简介:纪伟(1989-),男,江苏睢宁人,博士研究生,高级工程师,主要从事菌物资源研究与利用工作。(E-mail)jiwei100500@163.com通讯作者:任立凯,(E-mail)2949823@qq.com;陈克龙,(E-mail)ckl7813@163.com
更新日期/Last Update: 2024-03-17