[1]张雪花,王卫华,武奇,等.H9亚型禽流感广谱亚单位疫苗抗原设计及其免疫效力[J].江苏农业学报,2023,(09):1901-1907.[doi:doi:10.3969/j.issn.1000-4440.2023.09.012]
　ZHANG Xue-hua,WANG Wei-hua,WU Qi,et al.Antigen design and immune efficacy of a universal subunit vaccine against H9 subtype avian influenza[J].,2023,(09):1901-1907.[doi:doi:10.3969/j.issn.1000-4440.2023.09.012]
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H9亚型禽流感广谱亚单位疫苗抗原设计及其免疫效力()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年09期
页码:
1901-1907
栏目:
畜牧兽医·水产养殖
出版日期:
2023-12-31

文章信息/Info

Title:
Antigen design and immune efficacy of a universal subunit vaccine against H9 subtype avian influenza
作者:
张雪花1; 2; 王卫华1; 3; 武奇1; 2; 李丁1; 2; 平继辉3; 梅梅1; 2
(1.江苏省农业科学院动物免疫工程研究所/国家兽用生物制品工程技术研究中心/江苏省食品质量与安全重点实验室,江苏南京210014；2.兽用生物制品<泰州>国泰技术创新中心,江苏泰州225300；3.南京农业大学动物医学院,江苏南京210095)
Author(s):
ZHANG Xue-hua1; 2; WANG Wei-hua1; 3; WU Qi1; 2; LI Ding1; 2; PING Ji-hui3; MEI Mei1; 2
(1.Institute of Veterinary Immunology ＆ Engineering, Jiangsu Academy of Agricultural Sciences/National Research Center of Engineering and Technology for Veterinary Biologicals/Jiangsu Key Laboratory for Food Quality and Safety, Nanjing 210014, China；2.GuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou 225300, China；3.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
H9亚型禽流感; HA基因; 杆状病毒表达系统; 广谱亚单位疫苗; 免疫
Keywords:
H9 subtype avian influenza; HA gene; baculovirus expression system; broad-spectrum subunit vaccines; immunity
分类号:
S852.65
DOI:
doi:10.3969/j.issn.1000-4440.2023.09.012
文献标志码:
A
摘要:
H9N2亚型禽流感病毒(AIV)变异快、传染性强,由病毒流行株制备而成的灭活疫苗达不到完全保护的效果,导致H9N2亚型禽流感时有发生。为研发具有广谱保护性的H9亚型禽流感疫苗,本研究利用生物信息技术,对H9亚型AIV HA基因序列进行分析,设计获得HA基因序列。将HA基因构建于杆状病毒表达系统,表达的重组HA蛋白血凝效价为1×212,与不同分支H9亚型AIV阳性血清具有良好的交叉反应活性。将重组蛋白制备成疫苗(rHA疫苗)免疫10日龄雏鸡,免疫后28 d将不同亚分支的H9亚型AIV流行毒株115和YZ株分别以1×106EID50(半数鸡胚感染量)感染试验鸡,rHA疫苗组交叉保护效力明显优于商品苗组。本研究结果表明H9亚型禽流感广谱型亚单位疫苗具有较好的应用前景,可以用于防控H9 AIV感染。
Abstract:
The H9N2 subtype avian influenza virus (AIV) evolved rapidly with enhanced infectivity, and the inactivated vaccine prepared from the epidemic strain could not provide complete protective effect, which led to H9N2 AIV epidemics occurring frequently. In order to develop a broad-spectrum protective vaccine against H9 AIV, the HA gene sequences of H9 AIV epidemic strains were analyzed by biological information technology, and HA gene sequence was obtained. The HA gene was constructed into the baculovirus expression system, and the hemagglutination titer of rHA protein was 1×212. The rHA showed good cross-reactivity with the positive sera of different branches of H9 AIV. Ten-day-old chickens were immunized with rHA protein. After 28 days of immunization, testing chickens were infected with 1×106 EID50 of H9 subtype AIV strain 115 and strain YZ, respectively. The protective efficacy of rHA vaccine was obviously better than that of commercial vaccine group. The results suggest that the broad-spectrum subunit vaccine against H9 subtype AIV has the potential to be developed as universal vaccine that can protect against H9 AIV infection.

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备注/Memo

备注/Memo:
收稿日期:2023-03-23基金项目:江苏省农业科技自主创新基金项目[CX(22)3031]作者简介:张雪花(1982-),女,山东郓城人,硕士,助理研究员,主要从事动物免疫学及兽用生物制品研究。(E-mail)zhangxuehua1213@163.com通讯作者:梅梅,(E-mail)jacqui18@163.com
更新日期/Last Update: 2024-01-15