[1]杨盛茹,侯银臣,张秋双,等.超声波辅助提取黄粉虫中黄酮类物质的工艺优化及黄酮类物质的稳定性[J].江苏农业学报,2023,(01):229-237.[doi:doi:10.3969/j.issn.1000-4440.2023.01.026]
　YANG Sheng-ru,HOU Yin-chen,ZHANG Qiu-shuang,et al.Study on optimization of ultrasonic assisted extraction process and stability of flavonoids from Tenebrio molitor[J].,2023,(01):229-237.[doi:doi:10.3969/j.issn.1000-4440.2023.01.026]
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超声波辅助提取黄粉虫中黄酮类物质的工艺优化及黄酮类物质的稳定性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
229-237
栏目:
加工贮藏·质量安全
出版日期:
2023-02-28

文章信息/Info

Title:
Study on optimization of ultrasonic assisted extraction process and stability of flavonoids from Tenebrio molitor
作者:
杨盛茹1; 侯银臣1; 张秋双2; 潘春梅1; 李亚博1; 田腴澍1; 韩檀楚1
(1.河南牧业经济学院食品与生物工程学院,河南郑州450046;2.河南省兽药饲料监察所, 河南郑州450001)
Author(s):
YANG Sheng-ru1; HOU Yin-chen1; ZHANG Qiu-shuang2; PAN Chun-mei1; LI Ya-bo1; TIAN Yu-shu1; HAN Tan-chu1
(1.College of Food and Bioengineering Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China;2.Henan Province Institute of Veterinary Drug and Feed Control, Zhengzhou 450001, China)
关键词:
黄粉虫; 黄酮类物质; 超声波辅助; 稳定性
Keywords:
Tenebrio molitor; flavonoids; ultrasonic assistance; stability
分类号:
S899.9
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.026
文献标志码:
A
摘要:
以黄粉虫为原料，乙醇为提取剂，黄粉虫中黄酮类物质的提取率为指标, 采用超声波辅助-水浴加热的方法，在单因素（液料比、提取温度、提取时间、超声波功率和乙醇体积分数）试验的基础上设计响应面试验进行工艺优化，在不同条件（光照、温度、pH值、添加强氧化剂、添加还原剂和添加食品添加剂）下分析其稳定性。结果表明，黄粉虫中黄酮类物质最佳提取条件为:液料比50∶1（ml/g），提取温度60 ℃，提取时间41 min，超声波功率400 W，乙醇体积分数76%, 在该工艺条件下黄酮类物质的提取率为0.563 3%。稳定性分析结果表明，黄粉虫中的黄酮类物质受光照的影响较为明显，在过酸或过碱条件下会分解，在低含量（质量分数≤0.25%）强氧化剂作用下，黄酮类物质的稳定性良好，在一定范围内，还原剂亚硫酸钠与食品添加剂对其稳定性的影响不明显。研究结果可为黄粉虫中黄酮资源的开发提供理论依据。
Abstract:
Using Tenebrio molitor as raw material, ethanol as extraction agent, the extraction rate of flavonoids in Tenebrio molitor as index, the ultrasonic auxiliary-water bath heating method was adopted. Based on the single factor (liquid-to-material ratio, extraction temperature, extraction time, ultrasonic power and ethanol concentration) experiments, the response surface test was designed to optimize the process. Its stability was analyzed under different conditions (light, temperature, pH, strong oxidants, reducing agents and food additives). The optimal extraction conditions for flavonoids in mealworm were as follows: liquid-to-material ratio was 50∶1 (ml/g), extraction temperature was 60 ℃, extraction time was 41 min, ultrasonic power was 400 W, ethanol concentration was 76%. Under these conditions, the extraction rate of flavonoids was 0.563 3%. The results of stability analysis showed that the flavonoids in mealworm were more obviously affected by light, and would decompose under the condition of acid or alkali. Under the action of strong oxidant with low content (≤0.25%), the stability of flavonoids was good. In a certain range, the effect of reducing agent sodium sulfite and food additives on its stability was not obvious. These results can provide a theoretical basis for the development of flavonoid resources in Tenebrio molitor.

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备注/Memo

备注/Memo:
收稿日期：2022-11-04基金项目：河南省科技攻关项目(212102110313)；河南省重大公益专项项目(201300110300)；河南牧业经济学院食品科学与工程重点学科建设项目(Xjxk202203)作者简介：杨盛茹(1988-) 女，河南商丘人，博士研究生，讲师，主要研究方向为天然活性产物。(E-mail)81692@hnuahe.edu.cn通讯作者：侯银臣，(E-mail)307219505@qq.com
更新日期/Last Update: 2023-03-21