[1]杨春妹,李志明,范先婷,等.福林酚比色法测定海藻多酚的反应条件优化[J].江苏农业学报,2023,(01):238-245.[doi:doi:10.3969/j.issn.1000-4440.2023.01.027]
　YANG Chun-mei,LI Zhi-ming,FAN Xian-ting,et al.Study on determination conditions of seaweed polyphenols by Folin-Ciocalteu colorimetry[J].,2023,(01):238-245.[doi:doi:10.3969/j.issn.1000-4440.2023.01.027]
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福林酚比色法测定海藻多酚的反应条件优化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
238-245
栏目:
加工贮藏·质量安全
出版日期:
2023-02-28

文章信息/Info

Title:
Study on determination conditions of seaweed polyphenols by Folin-Ciocalteu colorimetry
作者:
杨春妹; 李志明; 范先婷; 沈宏
(华南农业大学资源环境学院，广东广州510642)
Author(s):
YANG Chun-mei; LI Zhi-ming; FAN Xian-ting; SHEN Hong
(College of Resource and Environment, South China Agricultural University, Guangzhou 510642, China)
关键词:
海藻多酚; 福林酚比色法; 海藻; 有机物质; 无机离子
Keywords:
seaweed polyphenols; Folin-Ciocalteu colorimetry; algae; organic matter; inorganic ions
分类号:
TS201.4
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.027
文献标志码:
A
摘要:
为明确福林酚比色法测定海藻多酚含量的反应条件,本研究采用单因素试验和正交试验相结合的方法，优化了福林酚用量、碳酸钠用量、反应时间、反应温度等福林酚法测海藻多酚含量的检测条件；并进一步分析了外源物质（有机物质、无机离子）对测定结果的影响。结果表明,应用福林酚比色法测定海藻多酚的最佳反应条件为：12%福林酚5 ml、3%碳酸钠4 ml、反应温度37 ℃、反应时间30 min、最佳吸收波长730 nm；该方法的加标回收率为96.70%-103.67%，相对标准差2.68%。优化的福林酚法测得7种藻类的多酚含量为120.0～273.0 mg/L，其测定结果与国家标准茶多酚检测法的检测结果一致。外源添加1%或5%的Cu2+、Ni2+、Mn2+、Cl-、I-、蛋白质、可溶性淀粉测定结果增加2.31%-149.79%;添加5%的Zn2+、CO2-3、NO-3、PO3-4测定结果显著降低，而山梨酸钾、葡萄糖、D-山梨醇对福林酚比色法检测海藻多酚含量无明显影响。本研究建立的海藻多酚检测法的稳定性、重现性以及加标回收率的相对标准差均低于3%，符合检测要求。
Abstract:
In order to clarify the reaction conditions for the determination of seaweed polyphenol content by Folin-Ciocalteu colorimetric method, single factor test and orthogonal test were used to optimize the amount and concentration of Folin phenol, the amount and concentration of sodium carbonate, the reaction time and the reaction temperature. The effects of exogenous substances (organic substances and inorganic ions) on the determination results were further analyzed. The results showed that the optimum conditions for the determination of seaweed polyphenols by Folin-Ciocalteu colorimetric method were 12% Folin phenol 5 ml, 3% sodium carbonate 4 ml, reaction temperature 37 ℃, reaction time 30 min and the best absorption wavelength 730 nm. The recovery of this method was 96.70%-103.67%, and the relative standard deviation was 2.68%. The polyphenol content of seven kinds of algae measured by the optimized Folin-Ciocalteu colorimetric method was 120.0-273.0 mg/L, which was consistent with the results of national standard tea polyphenol detection method. Exogenous addition of 1% or 5% Cu2+, Ni2+, Mn2+, Cl-, I-, protein, soluble starch would increase the determination results by 2.31%-149.79%. The addition of 5% Zn2+, CO2-3, NO-3, PO3-4 significantly reduced the determination results, while glycine, potassium sorbate, glucose and D-sorbitol had no significant effect on the determination of algae polyphenol content by Folin-Ciocalteu colorimetric method. The relative standard deviations of stability, reproducibility and the recovery of seaweed polyphenols detection method established in this study were all lower than 3%, which met the detection requirements.

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备注/Memo

备注/Memo:
收稿日期：2022-05-27基金项目：广东省基础与应用基础研究基金项目（2021A1515012113）；国家重点研发计划项目(2016YFD0200405-5)作者简介：杨春妹(1997-)，女，云南大理人，硕士研究生，主要从事海藻提取物相关方向的研究。(E-mail) 2057707404@qq.com通讯作者：沈宏，(E-mail) hshen@scau.edu.cn
更新日期/Last Update: 2023-03-21