[1]吴娥娇,郭文龙,王志伟,等.不同地域草莓拟盘多毛孢根腐病菌致病力差异及对苯醚甲环唑的敏感性[J].江苏农业学报,2023,(01):44-52.[doi:doi:10.3969/j.issn.1000-4440.2023.01.006]
　WU E-jiao,GUO Wen-long,WANG Zhi-wei,et al.Virulence difference and sensitivity analysis to difenoconazole of Neopestalotiopsis clavispora crown rot pathogens isolated from strawberry plants of different areas in China[J].,2023,(01):44-52.[doi:doi:10.3969/j.issn.1000-4440.2023.01.006]
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不同地域草莓拟盘多毛孢根腐病菌致病力差异及对苯醚甲环唑的敏感性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
44-52
栏目:
植物保护
出版日期:
2023-02-28

文章信息/Info

Title:
Virulence difference and sensitivity analysis to difenoconazole of Neopestalotiopsis clavispora crown rot pathogens isolated from strawberry plants of different areas in China
作者:
吴娥娇1; 郭文龙1; 2; 王志伟1; 2; 关玲1; 乔玉山1; 赵密珍1
(1.江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京210014；2.扬州大学园艺与植物保护学院，江苏扬州225009)
Author(s):
WU E-jiao1; GUO Wen-long1; 2; WANG Zhi-wei1; 2; GUAN Ling1; QIAO Yu-shan1; ZHAO Mi-zhen1
(1.Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China;2.College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China）
关键词:
草莓拟盘多毛孢根腐病菌; 苯醚甲环唑; 致病力; 敏感性
Keywords:
Neopestalotiopsis clavispora from strawberry crown root; difenoconazole; virulence; sensitivity
分类号:
S436.63
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.006
文献标志码:
A
摘要:
近年来中国草莓主产区拟盘多毛孢根腐病大面积发生并呈危害加重趋势。为明确不同地域来源拟盘多毛孢根腐病菌的致病力差异及其对苯醚甲环唑的敏感性,采用离体叶片和菌丝生长速率法对收集到的不同地域的73株病原菌菌株进行致病力分析和苯醚甲环唑敏感性测定。结果表明，不同地域来源的菌株致病力差异极显著，且和采样地年平均温度呈显著正相关。不同病原菌菌株对苯醚甲环唑的敏感性呈单峰分布，EC50范围为0.01～2.94 μg/ml，平均值为1.34 μg/ml。菌丝生长速率与AUDPC（病情发展曲线下面积）之间无相关性，不同苯醚甲环唑质量浓度下菌丝生长速率和产孢量之间的相关性存在差异。研究结果为苯醚甲环唑应用于草莓拟盘多毛孢根腐病的精准防控提供科学依据。
Abstract:
Strawberry crown rot caused by Neopestalotiopsis spp. has occurred in many areas in China and led to an increasing economic loss in recent years. In order to clarify the virulence difference of Neopestalotiopsis crown rot pathogens from different geographical sources and their sensitivity to difenoconazole, the pathogenicity analysis and sensitivity to difenoconazole of 73 pathogenic strains collected from different regions were determined by in vitro leaf and mycelial growth rate method. The results showed that the virulence of strains from different geographical sources was significantly different, and significantly positively correlated with the annual mean temperature of the sampling sites. The sensitivity of different pathogenic strains to difenoconazole showed a single peak distribution. The EC50 values of the 73 N. clavispora isolates ranged from 0.01 μg/ml to 2.94 μg/ml, with an average of 1.34 μg/ml. There was no correlation between mycelial growth rate and area under disease progress curve (AUDPC), and the correlation between mycelium growth rate and conidia production was different under different difenoconazole concentrations. The results provide a scientific basis for the application of difenoconazole in the precise prevention and control of strawberry Neopestalotiopsis crown rot.

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备注/Memo

备注/Memo:
收稿日期：2022-10-28基金项目：国家自然科学基金项目（32102337）；国家重点研发计划项目（2019YFD1000800）；江苏省农业科技自主创新基金项目[CX(21)2019]作者简介：吴娥娇（1988-），女，河南商丘人，博士，助理研究员，主要从事草莓病害流行与防控研究。（E-mail）20190028@jaas.ac.cn通讯作者：赵密珍，（E-mail）njzhaomz@163.com
更新日期/Last Update: 2023-03-21