[1]周玉飞,罗晓青,王晓敏,等.喀斯特地区珍稀濒危铁皮石斛野外回归试验[J].江苏农业学报,2022,38(03):798-805.[doi:doi:10.3969/j.issn.1000-4440.2022.03.027]
　ZHOU Yu-fei,LUO Xiao-qing,WANG Xiao-min,et al.Re-introduction experiment of rare and endangered Dendrobium officinale in karst area[J].,2022,38(03):798-805.[doi:doi:10.3969/j.issn.1000-4440.2022.03.027]
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喀斯特地区珍稀濒危铁皮石斛野外回归试验()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
798-805
栏目:
园艺
出版日期:
2022-06-30

文章信息/Info

Title:
Re-introduction experiment of rare and endangered Dendrobium officinale in karst area
作者:
周玉飞; 罗晓青; 王晓敏; 康专苗; 张显波
（贵州省农业科学院亚热带作物研究所，贵州兴义562400）
Author(s):
ZHOU Yu-fei;  LUO Xiao-qing;  WANG Xiao-min;  KANG Zhuan-miao;  ZHANG Xian-bo
(Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Xingyi 562400, China)
关键词:
铁皮石斛; 珍稀濒危; 喀斯特地区; 野外回归
Keywords:
Dendrobium officinale; rare and endangered; karst area; re-introduction
分类号:
S181
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.027
文献标志码:
A
摘要:
野外回归是珍稀濒危植物多样性保护的主要方式，将珍稀濒危植物铁皮石斛种质扩繁后回归到原生境以实现喀斯特地区铁皮石斛资源多样性保护。根据黔西南州喀斯特地区野生铁皮石斛资源原生境勘察情况，在野生铁皮石斛资源分布地兴义市则戎镇冷洞村开展铁皮石斛野外回归方式和生境条件研究，并监测回归后期铁皮石斛的适应性、生物学特性和生长情况。结果表明，铁皮石斛植于纵向堆砌岩石、植于岩石天然缝隙、植于横向堆砌岩石和捆绑于岩石的存活率分别为95.02%、91.22%、62.57%和37.59%；山脊、山鞍、山顶、山脚和山腰的铁皮石斛存活率分别为93.44%、91.81%、87.01%、84.07%和82.49%；铁皮石斛回归1年后逐渐适应野外生境，在回归2～3年内存活率变化不明显，回归4年后存活率明显下降；铁皮石斛回归初期发病率较高，为7.60%，回归1年后发病率变化不明显，回归4～5年后铁皮石斛老株多因生境变化出现腐烂现象；铁皮石斛回归初期高位芽萌发率较高，为8.47%，回归1年后高位芽萌发率明显降低，回归3年后基本不萌发高位芽；不同回归方式、生境条件和回归时间对铁皮石斛的分蘖率、新芽高、新芽粗没有明显影响。综上所述，回归方式和生境条件是影响铁皮石斛野外回归成败的主要因素，植于纵向堆砌岩石和岩石天然缝隙的铁皮石斛存活率最高，郁闭度、通风条件、苔藓层等生境因子对铁皮石斛的适应性和生长具有一定影响。
Abstract:
Re-introduction is the main way to protect the diversity of rare and endangered plants. In order to protect the diversity of Dendrobium officinale resources in karst areas, the rare and endangered Dendrobium officinale germplasm was bred and returned to its original habitat. According to the investigation of the original habitat of wild Dendrobium officinale resources in karst area of southwest Guizhou, the re-introdution method and habitat conditions of Dendrobium officinale were studied in Lengdong Village, Zerong Town, Xingyi City, where the wild Dendrobium resources were distributed. And the adaptability, biological characteristics and growth of Dendrobium officinale were monitored at the later stage of re-introdution.The results showed that the survival rates of Dendrobium officinale planted in longitudinal pile rocks, natural crack of rocks, transverse pile rocks and bound to rocks were 95.02%, 91.22%, 62.57% and 37.59%, respectively. Moreover, the survival rates of Dendrobium officinale at ridge, saddle, top, foot and mountainside were 93.44%, 91.81%, 87.01%, 84.07% and 82.49%, respectively. Dendrobium officinale gradually adapted to the habitat after one year of re-introduction, the survival rate did not change significantly after two or three years, but decreased significantly after four years. The incidence rate of Dendrobium officinale was 7.60% at the beginning of re-introduction, did not change significantly after one year, the old Dendrobium officinale was mostly tended to rot due to habitat changes after four or five years. Relatively high germination rate of high bud was 8.47% at the beginning of re-introduction, decreased significantly after one year, and high bud dmost did not germinate after three years. The tiller rate, new bud height and diameter of Dendrobium officinale were not affected by different methods, different habitats conditions and different years. In conclusion, re-introdution methods and habitats conditions are the main factors affecting the re-introduction success or failure of Dendrobium officinale. The survival rate of Dendrobium officinale planted in longitudinal piles rocks and natural crevices of rocks is the highest. The habitat factors such as canopy density, ventilation condition and moss layer have certain effects on the adaptability and growth of Dendrobium officinale.

参考文献/References:

[1]侯欢欢,安明态,金勇,等. 基于贵州省兰科植物丰富度与分布特征的保护优先区研究[J]. 中国野生植物资源, 2021, 40(1): 88-94.
[2]CHASE M W, CAMERON K M, FREUDENSTEIN J V, et al. An updated classification of Orchidaceae[J]. Botanical Journal of Linnean Society, 2015, 177(2): 151-174.
[3]REN H, JIAN S G, LIU H X, et al. Advances in the reintroduction of rare and endangered wild plant species[J]. Science China Life Sciences, 2014, 57(6): 603-609.
[4]MAUNDER M. Plant reintroduction: an overview[J]. Biodiversity and Conservation, 1992, 1(3): 51-61.
[5]REN H, ZHANG Q M, LU H F, et al. Wild plant species with extremely small populations require conservation and reintroduction in China[J]. Ambio, 2012, 41(8): 913-917.
[6]吴明开,刘作易,罗晓青. 贵州珍稀兰科植物[M]. 贵阳：贵州科技出版社, 2014.
[7]ZHANG S B, CHEN W Y, HUANG J L, et al. Orchild species richness along elevational and environmental gradients in Yunnan, China[J]. PLoS One, 2015, 10(11): e142621.
[8]陈芳. 基于GIS的贵州铁皮石斛气候适宜性研究[D]. 贵阳: 贵州大学, 2017.
[9]罗晓青,周玉飞,王晓敏,等. 贵州喀斯特地区铁皮石斛仿野生种植研究[J]. 中药材, 2021, 44(3): 15-19.
[10]MASCHINSKI J, HASKINS K E. Plant reintroduction in a changing climate: promises and perils[M]. Washington: Island Press, 2012.
[11]周翔,高江云. 珍稀濒危植物的回归: 理论和实践[J]. 生物多样性, 2011, 19(1): 97-105.
[12]陈宝玲,宋希强,余文刚,等. 濒危兰科植物再引入技术及其应用[J]. 生态学报, 2010, 30(24): 7055-7063.
[13]WRIGHT M, CROSS R, DIXON K, et al. Propagation and reintroduction of Caladenia[J]. Australian Journal of Botany, 2008, 57(4): 373-387.
[14]QUARMBY J P. Recovery plan for twelveth reatened orchids in the lofty block region of south Australia 2010：ISBN 1921238801 FIS90534 [S]. South Australia: Department for Environment and Natural Resource, 2010.
[15]REITER N, WHITFIELD J, POLLARD G, et al. Orchid re-introductions: an evaluation of success and ecological considerations using key comparative studies from Australia[J]. Plant Ecology, 2016, 217(1): 81-95.
[16]周志华,金效华. 中国野生植物保护管理的政策、法律制度分析和建议[J]. 生物多样性, 2021, 29(12): 1583-1590.
[17]刘仲健,刘可为,陈利君,等. 濒危物种杏黄兜兰的保育生态学[J]. 生态学报, 2006, 26(9): 2791-2800.
[18]周艳,冯佑鸿,李依蔓,等. 濒危植物白花兜兰野外回归研究[J]. 贵州科学, 2018, 36(5): 10-13.
[19]张哲. 海南三种蝴蝶兰属植物的保育生物学研究[D]. 海口：海南大学, 2018.
[20]刘锋,杨異,安明态,等. 贵州佛顶山国家级自然保护区兰科植物多样性及其保护研究[J]. 北方园艺, 2022,1(2): 62-70.
[21] EDWARD O, GUERRANT J, THOMAS N, et al. Reintroduction of rare and endangered plants: common factors, questions and approaches[J]. Australian Journal Botangy, 2007, 55(3): 362-370.
[22]王洁. 凹叶厚朴繁育系统研究及其濒危的生殖生物学原因分析[D]. 北京：中国林业科学研究院, 2012.
[23]王珊. 极危植物宝华玉兰繁殖生物学特性研究[D]. 南京：南京林业大学, 2020.
[24]张庆宝. 木兰属植物资源收集和天目木兰种苗特征与抗性研究[D]. 杭州：浙江林学院, 2009.
[25]赖彦池. 凹脉金花茶的保护遗传学研究[D]. 桂林：广西师范大学, 2018.
[26]张哲,任明迅,向文倩,等. 东南亚兰科植物的物种多样性、生活习性及其传粉系统[J]. 广西植物, 2021, 41(10): 1683-1698.
[27]高越,郭顺星,邢晓科. 兰科植物种子共生萌发真菌多样性及共生萌发机制研究进展[J]. 菌物学报, 2019, 38(11): 1808-1825.
[28]DING J T, TU H Y, ZANG Z L, et al. Precise control and prediction of the greenhouse growth environment of Dendrobium candidum[J]. Computers and Electronics in Agriculture, 2018, 151(8): 453-459.
[29]CORMICK M, BURNETT R, WHIGHAM D. Protocorm-supporting fungi are retained in roots of mature Tipularia discolor orchids as mycorrhizal fungal diversity increases[J]. Plants, 2021, 10(6): 1-13.
[30]QUENTIN F, LORENE G, SONIA L M, et al. Orchids and their mycorrhizal fungi: an insufficiently explored relationship[J]. Mycorrhiza, 2020, 1(30): 5-22.
[31]BRUNDRETT M C, TEDERSOO L. Evolutionary history of mycorrhizal symbioses and global host plant diversity[J]. New Phytologist， 2018, 220(4): 1108-1115.
[32]RAYBUM A P. Recognition and utilization of positive plant interactions may increase plant reintroduction success[J]. Biological Conservation, 2011, 144(5): 1296.

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备注/Memo

备注/Memo:
收稿日期：2022-02-28基金项目：贵州省科技计划项目[黔科合支撑（2020） 4Y011号、黔科合支撑（2021）一般227、黔科合重大专项（2019）3001]；贵州省科学技术基金项目[黔科合LH字（2014）7700号]作者简介：周玉飞（1984-），女，贵州兴义人， 硕士，助理研究员，研究方向为兰科植物保护与利用。（E-mail）411725823@qq.com
更新日期/Last Update: 2022-07-07