[1]周春宝,刘林雨,胡廷延,等.苏姜猪MC4R基因多态性及其与生产性状的关联分析[J].江苏农业学报,2021,(04):929-935.[doi:doi:10.3969/j.issn.1000-4440.2021.04.015]
　ZHOU Chun-bao,LIU Lin-yu,HU Ting-yan,et al.Analysis on MC4R gene polymorphism and its association with production traits in Sujiang pigs[J].,2021,(04):929-935.[doi:doi:10.3969/j.issn.1000-4440.2021.04.015]
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苏姜猪MC4R基因多态性及其与生产性状的关联分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年04期
页码:
929-935
栏目:
畜牧兽医·水产养殖
出版日期:
2021-08-28

文章信息/Info

Title:
Analysis on MC4R gene polymorphism and its association with production traits in Sujiang pigs
作者:
周春宝1; 刘林雨1; 胡廷延1; 2; 倪黎纲1; 徐盼1
（1.江苏农牧科技职业学院动物科技学院，江苏泰州225300；2.扬州大学动物科技学院，江苏扬州225009）
Author(s):
ZHOU Chun-bao1; LIU Lin-yu1; HU Ting-yan1; 2; NI Li-gang1; XU Pan1
(1.School of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;2.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China)
关键词:
苏姜猪; MC4R; 单核苷酸多态性; 生产性状
Keywords:
Sujiang pig; MC4R; single nucleotide polymorphism (SNP); production traits
分类号:
S858.28
DOI:
doi:10.3969/j.issn.1000-4440.2021.04.015
文献标志码:
A
摘要:
本研究旨在探讨MC4R基因多态性对苏姜猪生产性状的影响，以期能够筛选出可用于提高苏姜猪生产性状的分子标记。利用DNA混合池和Sanger测序法对MC4R基因外显子进行单核苷酸多态性（SNP）检测，分析SNP的遗传多态性及其与生产性状的关联性。结果显示，苏姜猪MC4R基因外显子中共检测到6个SNP位点，其中1个错义突变[rs81219178 G>A（Asp298Asn）]，5个3′UTR突变（rs325999553 G>A、rs344775772 T>A、rs334536177 A>C、rs335628164 C>T和rs81221063 A>T），均包含3种基因型，且符合Hardy-Weinberg平衡，2个SNP位点为低度多态，4个SNP位点为中度多态。关联分析结果显示，rs81219178 G>A(Asp298Asn)位点对胸围和胸宽有显著影响，5个3′UTR突变位点对体质量、体高和胸围有显著影响。rs325999553 G>A和rs344775772 T>A呈现高度连锁，rs334536177 A>C、rs335628164 C>T和rs81221063 A>T呈现高度连锁。结果提示，MC4R基因对苏姜猪的部分生产性状有显著影响，可作为苏姜猪早期选育的分子标记。
Abstract:
This study was aimed to explore the effect of MC4R gene polymorphism on the production traits of Sujiang pigs, as so to screen molecular markers that can be used to improve the production traits of Sujiang pigs. Single nucleotide polymorphism (SNP) in the exons of MC4R gene was detected by DNA pooling and Sanger sequencing to analyze the genetic polymorphism of SNP and its association with production traits. The results showed that six SNP loci were detected in the exons of MC4R gene of Sujiang pigs, including one missense mutation [rs81219178 G>A（Asp298Asn）], five 3′UTR mutations (rs325999553 G>A, rs344775772 T>A, rs334536177 A>C, rs335628164 C>T and rs81221063 A>T), which all contained three genotypes and conformed to Hardy-Weinberg equilibrium. Two SNP loci were low polymorphic and four SNP loci were moderate polymorphic. Association analysis results indicated that rs81219178 G>A(Asp298Asn) locus had significant effects on chest circumference and chest width, and five 3′UTR mutational loci had significant effects on body weight, body height and chest circumference. There showed high linkages between rs325999553 G>A and rs344775772 T>A, among rs334536177 A>C, rs335628164 C>T and rs81221063 A>T. The results suggest that MC4R gene has a significant effect on some production traits of Sujiang pigs and can be used as a molecular marker in early breeding of Sujiang pigs.

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备注/Memo

备注/Memo:
收稿日期：2021-01-08基金项目：国家自然科学基金项目（31702089）；江苏省第五期“333高层次人才培养工程”项目；江苏高校“青蓝工程”项目；江苏省大学生创新创业训练计划项目（202012806026Y）作者简介：周春宝（1973-），男，江苏高邮人，博士，教授，主要从事动物遗传育种与繁殖研究。(E-mail)zhou_tz@163.com。刘林雨为共同第一作者。通讯作者：徐盼，(E-mail)panxu_nj@hotmail.com
更新日期/Last Update: 2021-09-06