[1]孟佳丽,吴绍军,沈虹,等.不同药剂处理对西瓜连作土壤的影响[J].江苏农业学报,2021,(03):651-659.[doi:doi:10.3969/j.issn.1000-4440.2021.03.013]
　MENG Jia-li,WU Shao-jun,SHEN Hong,et al.Effects of different chemical treatments on continuous cropping soil of watermelon[J].,2021,(03):651-659.[doi:doi:10.3969/j.issn.1000-4440.2021.03.013]
点击复制

不同药剂处理对西瓜连作土壤的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年03期
页码:
651-659
栏目:
耕作栽培·资源环境
出版日期:
2021-06-30

文章信息/Info

Title:
Effects of different chemical treatments on continuous cropping soil of watermelon
作者:
孟佳丽; 吴绍军; 沈虹; 周玲玲; 张黎杰; 余翔
（江苏省农业科学院宿迁农业科学研究所，江苏宿迁223800）
Author(s):
MENG Jia-li; WU Shao-jun; SHEN Hong; ZHOU Ling-ling; ZHANG Li-jie; YU Xiang
（Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian 223800, China）
关键词:
土壤处理; 连作障碍; 微生物群落; 西瓜
Keywords:
soil treatment; continuous cropping obstacle; microbial community; watermelon
分类号:
S651
DOI:
doi:10.3969/j.issn.1000-4440.2021.03.013
文献标志码:
A
摘要:
以未经任何处理的种植1年西瓜的土壤为对照，采用高通量测序技术分析生石灰、敌克松、根宝、枯草芽孢杆菌、甲霜噁霉灵处理对西瓜连作土壤微生物群落的影响，并揭示土壤微生物群落与土壤养分含量及理化性质的相关性。结果表明，连作后土壤中的细菌菌群相对丰度降低，真菌菌群相对丰度增加。经过枯草芽孢杆菌处理的土壤中芽孢杆菌属菌群相对丰度提高。经过敌克松处理的土壤中水小杆菌属菌群相对丰度明显高于其他处理。连作后土壤中镰刀菌属真菌的比例上升。敌克松和根宝处理后，土壤中红菇属、曲霉属菌群相对丰度升高，镰刀菌属菌群相对丰度降低。芽孢杆菌属菌群相对丰度与铵态氮含量呈显著正相关，与土壤EC值呈显著负相关。类诺卡氏菌属菌群的相对丰度与土壤EC值呈极显著负相关。相对细菌菌群而言，真菌菌群受土壤理化性质影响较小。镰刀菌属真菌与土壤EC值呈显著正相关。说明，西瓜连作土壤采用杀菌剂处理或适量添加生物有机肥和微生物菌剂有利于土壤微生态环境的改善。
Abstract:
Taking the untreated soil in which watermelons were cultivated continuously for one-year as control, the effects of quicklime, Dixon, Genbo, Bacillus subtilis and metalaxyl-hymexazol on the microbial community in soils of continuous cropping watermelon were analyzed by high-throughput sequencing technology, and correlations between soil microbial community and soil nutrient content, physical and chemical properties were revealed. The results showed that, the relative abundance of bacterial flora in the soils decreased after continuous cropping, and the relative abundance of fungal flora increased. The relative abundance of Bacillus flora in the soils treated with Bacillus subtilis increased. The relative abundance of Aquabacterium flora in soils treated with Dixon was significantly higher than other treatments. The proportion of Fusarium fungi in the soil increased after continuous cropping. The relative abundance of Russula and Aspergillus floras increased and the relative abundance of Fusarium flora decreased in the soils treated with Dixon and Genbo. The relative abundance of Bacillus flora in the soils was in significant positive correlation with ammonium nitrogen content, and was in significant negative correlation with soil EC value. The relative abundance of Nocardioides flora was in extreme significant negative correlation with soil EC value. Compared with the bacterial flora, the fungal flora was less affected by the physical and chemical properties of the soils. The abundance of Fusarium community was in significant positive correlation with soil EC value. In the continuously cropping soils of watermelon, the soil micro-ecological environment was improved by fungicides treatment or appropriate bio-organic fertilizer and microbial agent treatment.

参考文献/References:

[1]陈名蔚,王峰,韩益飞,等.蔬菜连作障碍成因分析和防止技术初探[J].南方农业,2017,11(30):3-4.
[2]姜永.蔬菜生产连作障碍的发生与综合防治措施[J].吉林蔬菜,2013(7):26-27.
[3]唐丽雪,冯桂芳,丁原书,等.大棚蔬菜根结线虫病害调查研究以及防治对策[J].科技视界,2016(7):52，79.
[4]王光飞,高晓东,马艳,等.生物有机类复合调理剂在设施叶菜障碍土壤上的应用效果[J].中国土壤与肥料,2020(2):56-65.
[5]孙岩,刘学东,侯秀明,等.作物自毒作用研究和防治技术[J].上海蔬菜,2020(3):88-89.
[6]王军芳.设施蔬菜土壤环境恶化原因及防治对策[J].农业开发与装备,2019(8):170-171.
[7]LI R F, XU Y. Fermentation optimization to improve production of antagonistic metabolites by Bacillus subtilis strain BS501a[J]. Journal of Central South University of Technology,2011(18): 1047-1053.
[8]何世朋,梁斌,武德军,等.设施菜地番茄的养分需求规律研究[J/OL].华北农学报，2020，35（5）61-67
[2020-10-12].http://kns.cnki.net/kcms/detail/13.1101.S.20200730.1329.002.html.
[9]ANDRE P M,ANDREA K B,JAKUB M T,et al. PANDAseq: paired-end assembler for illumina sequences[J]. BMC Bioinformatics, 2012,13:31-38.
[10]EDGAR R. Taxonomy annotation and guide tree errors in 16S rRNA databases.[J]. Peer J,2018,6(4):e5030.
[11]BRIAN D O,NICHOLAS H B,ADAM M P. Interactive metagenomic visualization in a Web browser[J]. BMC Bioinformatics,2011,12（1）:385-392.
[12]DAVIDE B,RUBEN G O,PHILIPP C M,et al. Structure and function of the bacterial root microbiota in wild and domesticated barley[J]. Cell Host & Microbe,2015,17(3):392-403.
[13]ROBERT S,ROBERT E. Quality control and preprocessing of metagenomic datasets[J]. Bioinformatics,2011,27(6):863-864.
[14]NGUYEN M T,RANAMUKHAARACHCHI S L. Soil-borne antagonists for biological control of bacterial wilt disease caused by ralstonia solanacearum in tomato and pepper[J]. Journal of Plant Pathology,2010,92(2):395-405.
[15]甄文超,曹克强,胡同乐,等.作物再植病害的研究进展[J].河北农业大学学报,2001，24(4):98-103.
[16]李琼芳.不同连作年限麦冬根际微生物区系动态研究[J].土壤通报,2006，37(3):563-565.
[17]李晶晶,续勇波.百合连作年限对设施土壤理化性质和生物学特性的影响[J].土壤通报,2019,50(5):1171-1177.
[18]李坤,郭修武,孙英妮,等.葡萄连作对土壤细菌和真菌种群的影响[J].应用生态学报,2009,20(12):3109-3114.
[19]邹春娇,齐明芳,马建,等.Biolog-ECO解析黄瓜连作营养基质中微生物群落结构多样性特征[J].中国农业科学,2016,49(5):942-951.
[20] JIE W , BAI L , YU W , et al. Analysis of interspecific relationships between Funneliformis mosseae and Fusarium oxysporum in the continuous cropping of soybean rhizosphere soil during the branching period[J]. Biocontrol Science and Technology,2015,25(9):1036-1051.
[21]张志敏,赵政阳,樊红科,等.苹果根区土壤镰刀菌对再植砧木苗生长的影响[J].干旱地区农业研究,2009,27(2):204-207，217.
[22]刘中良,高俊杰,谷端银,等.有机肥对设施番茄周年栽培土壤环境和产量的影响[J].应用生态学报,2020,31(3):929-934.
[23]沐婵,钱荣青,王海波,等.全水溶性有机肥对设施连作种植甜瓜产量及产值的影响[J].农业科技通讯,2020(8):126-128.
[24]张晓颖.生物质炭缓解桃连作障碍的效应研究[D].武汉：华中农业大学,2013.
[25]张斌, 杨晓云, 陈志谊. 解淀粉芽孢杆菌生防菌B1619对设施番茄枯萎病的防治效果[C]//中国植物保护学会. 病虫害绿色防控与农产品质量安全——中国植物保护学会2015年学术年会论文集.长春：中国农业科学技术出版社，2015.
[26]KENNEDY A C, SMITH K L. Soil microbial diversity and the sustainability of agricultural soils[J]. Plant and Soil,1995,170(1)：75-86.
[27]DANIEL H B,THOMAS M S. Diversity and dynamics of microbial communities in soils from agro-ecosystems[J]. Environmental Microbiology,2003,5(6)：441-521.
[28]徐胜涛,王攀磊,何翔，等. 植物覆盖对蕉园土壤质量和微生物多样性的影响[J].南方农业学报，2020，51(3）：496-504.
[29]张变华,靳东升,郜春花,等. 不同方法分析工矿复垦区不同植物根际微生物多样性的比较[J]. 江苏农业科学,2019,47(4):223-226.
[30]彭玉娇, 崔学宇, 谭梦超，等. 不同立地条件沙田柚果园土壤微生物多样性分析[J].南方农业学报，2020，51(5):1136-1144.
[31]林峰，杨殿林，王华玲，等. 猕猴桃园生草对土壤微生物多样性的影响［J］．江苏农业科学，2020,48(10):293-297.
[32]SAMANTA B C,BRUNO B L,FLAVIO A O，et al. Soil suppressiveness and its relations with the microbial community in a Brazilian subtropical agroecosystem under different management systems[J]. Soil Biology and Biochemistry,2016,96:191-197.
[33]叶雯,李永春,喻卫武,等.不同种植年限香榧根际土壤微生物多样性[J].应用生态学报,2018,29(11):3783-3792.
[34]吴朝晖,刘清术,孙继民,等.基于高通量测序的超级稻不同生育期土壤细菌和古菌群落动态变化[J].农业现代化研究,2018,39(2):342-351.
[35]陈孟立,曾全超,黄懿梅,等.黄土丘陵区退耕还林还草对土壤细菌群落结构的影响[J].环境科学,2018,39(4):1824-1832.

相似文献/References:

[1]周开胜.厌氧还原土壤灭菌法抑制西瓜专化型尖孢镰刀菌[J].江苏农业学报,2015,(05):1006.[doi:doi:10.3969/j.issn.1000-4440.2015.05.010]
　ZHOU Kai-sheng.Anaerobic soil disinfestation, an effective way to control watermelon fusarium wilt caused by Fusarium oxysporum f. sp. niveum[J].,2015,(03):1006.[doi:doi:10.3969/j.issn.1000-4440.2015.05.010]
[2]刘勇,刘燕,胡茂飞,等.头花蓼水浸提液的化感自毒作用[J].江苏农业学报,2018,(03):520.[doi:doi:10.3969/j.issn.1000-4440.2018.03.006]
　LIU Yong,LIU Yan,HU Mao-fei,et al.Research on allelopathic autotoxicity of water extracts of Polygonum capitatum[J].,2018,(03):520.[doi:doi:10.3969/j.issn.1000-4440.2018.03.006]
[3]徐彬,徐健,祁建杭,等.江苏省设施蔬菜连作障碍土壤理化及生物特征[J].江苏农业学报,2019,(05):1124.[doi:doi:10.3969/j.issn.1000-4440.2019.05.018]
　XU Bin,XU Jian,QI Jian-hang,et al.Soil physical, chemical and biological characteristic analysis of long-term vegetable monoculture in greenhouse in Jiangsu province[J].,2019,(03):1124.[doi:doi:10.3969/j.issn.1000-4440.2019.05.018]
[4]马澜,邱黛玉,巫蓉,等.栽培模式对党参根际土壤微生物功能多样性的影响[J].江苏农业学报,2023,(05):1132.[doi:doi:10.3969/j.issn.1000-4440.2023.05.005]
　MA Lan,QIU Dai-yu,WU Rong,et al.Effects of cultivation modes on microbial functional diversity in the rhizosphere soil of Codonopsis pilosula[J].,2023,(03):1132.[doi:doi:10.3969/j.issn.1000-4440.2023.05.005]
[5]宋燕芳,彭彤,马少兰,等.枸杞叶片酚酸类物质的自毒作用分析[J].江苏农业学报,2024,(02):213.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]
　SONG Yan-fang,PENG Tong,MA Shao-lan,et al.Analyzing the autotoxicity of phenolic acids from Lycium barbarum L. leaves[J].,2024,(03):213.[doi:doi:10.3969/j.issn.1000-4440.2024.02.003]
[6]刘德才,殷剑美,金林,等.轮作蒜(青蒜)对大棚连作芋产量、品质及土壤微生物群落的影响[J].江苏农业学报,2024,(11):2040.[doi:doi:10.3969/j.issn.1000-4440.2024.11.007]
　LIU Decai,YIN Jianmei,JIN Lin,et al.Effects of garlic (garlic sprouts) rotation on taro yield, quality and soil microbial community in plastic tunnel[J].,2024,(03):2040.[doi:doi:10.3969/j.issn.1000-4440.2024.11.007]

备注/Memo

备注/Memo:
收稿日期：2020-10-13基金项目：江苏省宿迁市农业科技自主创新基金项目（SQCX202009）；江苏省农业重大新品种创制项目（PZCA201716）作者简介：孟佳丽（1987-），女，江苏徐州人，硕士研究生，主要从事西甜瓜育种与栽培技术研究。（Tel）18800609680；（E-mail）jiali1415118@163.com通讯作者：余翔，（Tel）15850917070；（E-mail）yx003@163.com
更新日期/Last Update: 2021-07-05