[1]赵亮,狄佳春,陈旭升.棉花基因组中赤霉素氧化酶基因的鉴定与分析[J].江苏农业学报,2020,(03):553-560.[doi:doi:10.3969/j.issn.1000-4440.2020.03.004]
 ZHAO Liang,DI Jia-chun,CHEN Xu-sheng.Identification and analysis of gibberellin oxidase gene in cotton genome[J].,2020,(03):553-560.[doi:doi:10.3969/j.issn.1000-4440.2020.03.004]
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棉花基因组中赤霉素氧化酶基因的鉴定与分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
553-560
栏目:
遗传育种·生理生化
出版日期:
2020-06-30

文章信息/Info

Title:
Identification and analysis of gibberellin oxidase gene in cotton genome
作者:
赵亮狄佳春陈旭升
(江苏省农业科学院经济作物研究所/农业农村部长江下游棉花与油菜重点实验室,江苏南京210014)
Author(s):
ZHAO LiangDI Jia-chunCHEN Xu-sheng
(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/ Key Laboratory of Cotton and Canola Research at the Lower Reach of the Yangtze River Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China)
关键词:
棉花赤霉素赤霉素氧化酶基因家族
Keywords:
cottongibberellic acidgibberellin oxidasegene family
分类号:
S562.01
DOI:
doi:10.3969/j.issn.1000-4440.2020.03.004
文献标志码:
A
摘要:
根据赤霉素氧化酶氨基酸序列的保守结构域和生物学信息,对棉花2个二倍体野生种(A基因组的亚洲棉和D基因组的雷蒙德氏棉)和2个异源四倍体遗传标准系(陆地棉TM-1和海岛棉H7124)进行了全基因组的查找。结果表明,在2个二倍体野生种的A和D基因组中分别预测到219和188个该酶类基因家族成员;在异源四倍体陆地棉TM-1和H7124中分别预测到了310和428个该酶类基因家族成员。通过分析陆地棉中预测到的310个该酶类基因家族成员在赤霉素敏感超矮突变体和野生型转录组中表达差异,发现16个基因具有显著性差异。利用这16个基因编码的蛋白质氨基酸序列与报道的拟南芥赤霉素氧化酶氨基酸序列进行进化分析,结果表明Gh_D06G2009属于GA3ox氧化酶,Gh_D01G0300和Gh_D09G0746为 GA2ox氧化酶,Gh_A06G1341、Gh_A07G1653、Gh_A11G1416、Gh_A13G0444、Gh_A13G1787、Gh_A13G2343、Gh_D01G0055、Gh_D07G0446、Gh_D07G1858、Gh_D08G2680、Gh_D11G3415、Gh_D13G0516、Gh_D13G2157为GA20ox氧化酶。
Abstract:
Based on the conserved domain of gibberellin oxidase amino acid sequence and bioinformatics, genome-wide searches were performed on two diploid wild cotton species (Gossypium arboreum with A genome and G. Raimondii with D genome) and two allotetraploid genetic standard lines (upland cotton TM-1 and island cotton H7124). In the two diploid wild species, 219 and 188 members of the enzyme family were predicted in the A and D genomes, respectively. In addition, 310 and 428 members of the enzyme family were predicted in allotetraploid upland cotton TM-1 and H7124, respectively. Subsequent analysis of 310 members predicted by us in upland cotton revealed significant differences in 16 genes between gibberellin mutants and wild-type transcriptomes. The amino acid sequence of the protein encoded by these 16 genes and the amino acid sequence of gibberellin oxidase in Arabidopsis thaliana were analyzed, it was found that Gh_D06G2009 could belong to GA3ox oxidase. Gh_D01G0300 and Gh_D09G0746 were GA2ox oxidase. However, Gh_A06G1341, Gh_A07G1653, Gh_A11G1416, Gh_A13G0444, Gh_A13G1787, Gh_A13G2343, Gh_D01G0055, Gh_D07G0446, Gh_D07G1858, Gh_D08G2680, Gh_D11G3415, Gh_D13G0516, Gh_D13G2157 were GA20ox oxidase.

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备注/Memo

备注/Memo:
收稿日期:2019-12-13基金项目:国家自然科学基金青年科学基金项目(31401429);江苏省自然科学基金面上基金项目(BK20181245)作者简介:赵亮(1984-),男,吉林农安人,博士研究生,副研究员,主要从事棉花分子生物学研究。(E-mail)Liangz@jaas.ac.cn通讯作者:陈旭升, (E-mail)njcxs@126.com
更新日期/Last Update: 2020-07-14