[1]曲继松,张丽娟,朱倩楠,等.水分胁迫对柠条基质栽培黄瓜幼苗生长及光合特性的影响[J].江苏农业学报,2019,(02):384-390.[doi:doi:10.3969/j.issn.1000-4440.2019.02.020]
　QU Ji-song,ZHANG Li-juan,ZHU Qian-nan,et al.Effects of water stress on growth and photosynthetic characteristics of cucumber seedlings under substrate culture of Caragana korshinskii[J].,2019,(02):384-390.[doi:doi:10.3969/j.issn.1000-4440.2019.02.020]
点击复制

水分胁迫对柠条基质栽培黄瓜幼苗生长及光合特性的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年02期
页码:
384-390
栏目:
园艺
出版日期:
2019-04-30

文章信息/Info

Title:
Effects of water stress on growth and photosynthetic characteristics of cucumber seedlings under substrate culture of Caragana korshinskii
作者:
曲继松1; 2; 张丽娟2; 朱倩楠2; 田永强1; 高丽红1
（1.中国农业大学园艺学院，北京100093；2.宁夏农林科学院种质资源研究所，宁夏银川750002）
Author(s):
QU Ji-song1; 2; ZHANG Li-juan2; ZHU Qian-nan2; TIAN Yong-qiang1; GAO Li-hong1
（1.College of Horticulture, China Agricultural University, Beijing 100093, China；2.Institute of Germplasm Resources, Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China）
关键词:
柠条基质; 黄瓜; 育苗; 水分生理; 光合作用
Keywords:
Caragana korshinskii growing matrix; cucumber; seedling; water physiology; photosynthesis
分类号:
S642.2
DOI:
doi:10.3969/j.issn.1000-4440.2019.02.020
文献标志码:
A
摘要:
研究水分胁迫对柠条基质栽培黄瓜幼苗光合作用及相关生理指标的影响，为利用新型园艺基质培育优质黄瓜幼苗提供理论参考。采用72穴标准穴盘进行育苗，测定正常供水（CK）、中度水分胁迫（5%PEG-6000，DR-M）和重度水分胁迫（10%PEG-6000，DR-S）处理下黄瓜幼苗的株高、茎粗、根冠比、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、光合参数、叶绿素荧光参数的变化。结果表明，水分胁迫显著抑制了黄瓜幼苗的生长，处理后第17 d，与CK相比，DR-M处理的株高降低8.30%，DR-S处理的株高降低16.20%，DR-M处理的黄瓜幼苗茎粗增加0.85%，DR-S处理的茎粗下降7.67%。与CK相比，DR-M处理下黄瓜幼苗干物质积累量增加，根冠比下降，叶绿素含量降低，丙二醛含量增加，SOD和POD活性升高，净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）、胞间CO2浓度（Ci）、水分利用效率(WUE)、初始荧光（Fo）、最大荧光（Fm）、可变荧光（Fv）、单位反应中心捕获的用于电子传递的能量(ETo/RC)均下降。对夏季柠条基质培育黄瓜幼苗而言，中度水分胁迫处理可抑制因温度过高、水分充足引起的徒长，有利于提高抗逆能力，增加干物质积累量，培育优质壮苗。
Abstract:
The effects of water stress on photosynthetic characteristics and related physiological indices of cucumber seedlings under substrate culture of Caragana korshinskii was studied to provide theoretical reference for the cultivation of high quality cucumber seedlings by using new horticultural substrate. 72-well standard plug trays were used for nursery. Changes of plant height, stem diameter, root-shoot ratio, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, peroxidase (POD） activity, photosynthetic parameters, and chlorophyll fluorescence parameters of cucumber seedlings were studied under normal water supply (CK), moderate water stress (5% PEG-6000, DR-M) and severe water stress (10% PEG-6000, DR-S) conditions. The results showed that the water stress significantly inhibited the growth of cucumber seedlings. On the 17th day of treatment, the plant height in the treatments of DR-M and DR-S decreased by 8.30% and 16.20% compared with that in CK. Moreover, compared with that in CK, stem diameter in the treatment of DR-M increased by 0.85%, and stem diameter in the treatment of DR-S decreased by 7.67%. Compared with those in the treatment of CK, the accumulation of dry matter in cucumber seedlings increased, the root-shoot ratio decreased, the content of chlorophyll decreased, the content of MDA increased, the activities of SOD and POD increased, the net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and water use efficiency (WUE), the basic parameters of chlorophyll fluorescence(Fo, Fm, Fv), captured energy for electron transfer (ETo/RC) decreased in the treatment of DR-M. Moderate water stress can inhibit legume growth caused by excessive temperature and sufficient water in the cultivation of cucumber seedlings under substrate culture of Caragana korshinskii in the summer, and it is beneficial to improve resistance to stress, increase dry matter content, and cultivate high-quality and strong seedlings.

参考文献/References:

[1]张曼义,杨再强,侯梦媛. 水分胁迫下黄瓜叶片光响应过程的模拟[J]. 中国农业气象, 2017, 38(10):644-654.
[2]曲继松,郭文忠,张丽娟,等. 柠条粉作基质对西瓜幼苗生长发育及干物质积累的影响[J]. 农业工程学报, 2010, 26(8):291-295.
[3]张丽娟,曲继松,冯海萍,等. 利用柠条粉发酵料作为育苗基质对甜瓜幼苗质量的影响[J]. 北方园艺, 2010(15):165-167.
[4]曲继松,张丽娟,冯海萍,等. 混配柠条复合基质对茄子幼苗生长发育的影响[J]. 西北农业学报,2012,21(11):162-167.
[5]曲继松,张丽娟,冯海萍,等. 发酵柠条粉混配基质对辣椒幼苗生长发育的影响[J]. 江苏农业学报,2012,28(4):846-850.
[6]董绪兵,毕焕改,刘业霞,等. 黄瓜幼苗干旱-低温交叉适应与渗透调节的关系[J].中国农业科学,2011,44(2):335-340.
[7]李晨,李秀杰,韩真,等. 非生物胁迫对葡萄光合作用的影响研究进展[J]. 山东农业科学，2017，49（12）：144-148.
[8]翁亚伟,张磊,张姗,等.盐旱复合胁迫对小麦幼苗生长和水分吸收的影响[J].生态学报,2017,37(7):2244-2252.
[9]马梦茹，王占林，贺康宁，等. 不同土壤含水量与光照对山杏和四翅滨藜光合作用的影响[J]. 江苏农业科学，2017，45（22）：126-129.
[10]JEYARAMRAJA P R, MEENAKSHI S N, JOSHI S D, et al. Water deficit induced oxidative damage in tea (Camellia sinensis) plants[J]. Journal of Plant Physiology,2005,162: 413-419.
[11]李佳，刘立云，李艳，等. 保水剂对干旱胁迫下槟榔幼苗生理特征的影响[J]. 南方农业学报，2018，49（1）：104-108.
[12]张志焕,韩敏,张逸,等.水分胁迫对不同抗旱性砧木嫁接番茄生长发育及水气交换参数的影响[J].中国农业科学,2017,50(2):391-398.
[13]桑子阳,马履一,陈发菊.干旱胁迫对红花玉兰幼苗生长和生理特性的影响[J].西北植物学报,2011,31(1): 109-115.
[14]牛铁泉,田给林,薛仿正,等.半根及半根交替水分胁迫对苹果幼苗光合作用的影响[J].中国农业科学,2007,40(7):1463-1468.
[15]睢晓蕾,毛胜利,王立浩,等.弱光条件下辣椒幼苗叶片的气体交换和叶绿素荧光特性[J].园艺学报,2007,34(31):615-622.
[16]MAXWELL K, JOHNSON G N. Chlorophyll fluorescence——a practical guide[J]. Journal of Experimental Botany,2000,51(345): 659-668.
[17]吴顺,张雪芹,蔡燕.旱胁迫对黄瓜幼苗叶绿素含量和光合特性的影响[J].中国农学通报,2014,30(1):133-137.
[18]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[19]李鹏民,高辉远,RETO J S. 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理学与分子生物学学报,2005,31(6):559-566.
[20]肖姣娣.不同强度干旱胁迫对刺槐幼苗生理生化特性的影响[J].中南林业科技大学学报,2015,35(8):23-26.
[21]ZHOU S, LI M, GUAN Q, et al. Physiological and proteome analysis suggest critical roles for the photosynthetic system for high water-use efficiency under drought stress in Malus[J]. Plant Science,2015,236: 44-60.
[22]高玉红,牛俊义,徐锐,等. 不同覆膜方式对玉米叶片光合、蒸腾及水分利用效率的影响[J]. 草业学报,2012,21(5):178-184.
[23]于文颖,纪瑞鹏,冯锐,等. 不同生育期玉米叶片光合特性及水分利用效率对水分胁迫的响应[J]. 生态学报,2015,35(9):2902-2909.
[24]孟凡超,张佳华,郝翠,等.CO2浓度升高和不同灌溉量对东北玉米光合特性及产量的影响[J].生态学报,2015,35(7):2126-2135.
[25]GALL A, HALDIMANN P, FELLER U. Photosynthetic performance and water relations in young pubescent oak (Quercus pubescens) trees during drought stress and recovery[J]. New Phytologist,2007,174(4): 799-810.
[26]FLEXAS J, BOTA J, GALMES J, et al. Keeping a positive carbon balance under adverse conditions: responses of photosynthesis and respiration to water stress[J]. Physiologia Plantarum,2006,127(3): 343-352.
[27]孙东宝,王庆锁. 水分对苜蓿叶片光合特性的影响[J]. 植物生态学报,2012,36(1):72-80.
[28]ZHANG G C, XIA J B, SHAO H B, et al. Grading woodland soil water productivity and soil bioavailability in the semi-arid loess plateau of China[J].Clean-Soil Air Water,2012,40(2): 148-153.
[29]SAUD S, YAJUN C, FAHAD S, et al. Silicate application increases the photosynthesis and its associated metabolic activities in Kentucky bluegrass under drought stress and post-drought recovery[J]. Environmental Science and Pollution Research,2016,23(17): 17647-17655.

相似文献/References:

[1]梁郸娜,胡其靖,曹 磊,等.蚜虫侵染对黄瓜叶片中丙二醛含量及保护酶活性的影响[J].江苏农业学报,2016,(02):278.[doi:10.3969/j.issn.1000-4440.2016.02.007]
　LIANG Dan-na,HU Qi-jing,CAO Lei,et al.Effects of aphid(Aphis gossypii Glover)infestation on MDA content and protective enzymes activities in cucumber[J].,2016,(02):278.[doi:10.3969/j.issn.1000-4440.2016.02.007]
[2]顾大路,王伟中,孙爱侠,等.不同轮作模式对日光温室黄瓜生长的影响[J].江苏农业学报,2016,(04):874.[doi:10.3969/j.issn.100-4440.2016.04.025]
　GU Da-lu,WANG Wei-zhong,SUN Ai-xia,et al.Cucumber growth in solar greenhouse affected by rotation modes[J].,2016,(02):874.[doi:10.3969/j.issn.100-4440.2016.04.025]
[3]马晓燕,高艳明,李建设.容器对基质栽培春茬黄瓜生长和产量的影响[J].江苏农业学报,2016,(04):879.[doi:10.3969/j.issn.100-4440.2016.04.026]
　MA Xiao-yan,GAO Yan-ming,LI Jian-she.Influences of containers on growth and yield of substrate-cultured spring cucumber[J].,2016,(02):879.[doi:10.3969/j.issn.100-4440.2016.04.026]
[4]张微微,潘俊松,蒋苏,等.黄瓜 RIL 群体侧枝相关性状 QTL 定位[J].江苏农业学报,2017,(01):174.[doi:10.3969/j.issn.1000-4440.2017.01.028]
　ZHANG Wei-wei,PAN Jun-song,JIANG Su,et al.Genetic mapping of QTL associated with lateral branch-related traits using recombinant inbred lines population in cucumber (Cucumis sativus L.)[J].,2017,(02):174.[doi:10.3969/j.issn.1000-4440.2017.01.028]
[5]徐心诚.弱光对温室黄瓜叶片和茎中可溶性糖含量的影响[J].江苏农业学报,2015,(06):1448.[doi:doi:10.3969/j.issn.1000-4440.2015.06.040]
　XU Xin-cheng.Soluble sugar contents in leaves and stems of greenhouse cucumber exposed to weak light[J].,2015,(02):1448.[doi:doi:10.3969/j.issn.1000-4440.2015.06.040]
[6]柯希欢,李霞,张琛,等.菇-热-酶-肥新型栽培方式对大棚黄瓜形态、生理和产量的影响[J].江苏农业学报,2015,(04):877.[doi:10.3969/j.issn.1000-4440.2015.04.026]
　KE Xi-huan,LI Xia,ZHANG Chen,et al.Effects of mushroom-heat-enzyme-fertilizer on the morphology, physiology and yield of greenhouse cucumber rotated with straw mushroom[J].,2015,(02):877.[doi:10.3969/j.issn.1000-4440.2015.04.026]
[7]李建设,刘宏久,郑佳琦,等.灌溉时间和灌水量对黄瓜穴盘苗生长及生理的影响[J].江苏农业学报,2015,(02):401.[doi:10.3969/j.issn.1000-4440.2015.02.028]
　LI Jian-she,LIU Hong-jiu,ZHENG Jia-qi,et al.Cucumber seedling growth and physiology influenced by irrigation time and amount[J].,2015,(02):401.[doi:10.3969/j.issn.1000-4440.2015.02.028]
[8]陈夕军,朱键鑫,陈羽,等.抗白粉病黄瓜品种的叶片组织结构及其生理生化[J].江苏农业学报,2015,(01):55.[doi:10.3969/j.issn.1000-4440.2015.01.008]
　CHEN Xi-jun,ZHU Jian-xin,CHEN Yu,et al.Physiological and biochemical mechanism of cucumber cultivars resistant to powdery mildew and their differences in microstructure of leaves[J].,2015,(02):55.[doi:10.3969/j.issn.1000-4440.2015.01.008]
[9]徐刚,彭天沁,高文瑞,等.氮肥水平对木薯渣复合基质栽培黄瓜生长及光合作用的影响[J].江苏农业学报,2015,(01):68.[doi:10.3969/j.issn.1000-4440.2015.01.010]
　XU Gang,PENG Tian-qin,GAO Wen-rui,et al.Effects of nitrogen fertilizer rates on growth and photosynthesis of cucumber in cassava compound substrate[J].,2015,(02):68.[doi:10.3969/j.issn.1000-4440.2015.01.010]
[10]曹玉杰,薄凯亮,程春燕,等.外源尸胺增强黄瓜幼苗耐冷性的生理机制[J].江苏农业学报,2015,(01):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]
　CAO Yu-jie,BO Kai-liang,CHENG Chun-yan,et al.Physiological mechanism of exogenous cadaverine enhancing the chilling tolerance of cucumber seedlings[J].,2015,(02):122.[doi:10.3969/j.issn.1000-4440.2015.01.019]

备注/Memo

备注/Memo:
收稿日期：2018-07-26 基金项目：公益性行业(农业)科研专项资助项目(201503137)；宁夏“十三五”重点研发计划项目(2016BZ0902、2017BN0103) 作者简介：曲继松（1980-），男，吉林永吉人，博士研究生，副研究员，主要从事设施环境调控和生物质基质化利用研究。（E-mail）qujs119@126.com 通讯作者：高丽红，（E-mail）gaolh@cau.edu.cn
更新日期/Last Update: 2019-05-05