[1]郭佳琪,杨晓宇,冯宇,等.猪非典型性瘟病毒RT-PCR检测方法的建立及初步应用[J].江苏农业学报,2019,(02):357-362.[doi:doi:10.3969/j.issn.1000-4440.2019.02.016]
　GUO Jia-qi,YANG Xiao-yu,FENG Yu,et al.Establishment and preliminary application of RT-PCR detection method for atypical porcine pestivirus[J].,2019,(02):357-362.[doi:doi:10.3969/j.issn.1000-4440.2019.02.016]
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猪非典型性瘟病毒RT-PCR检测方法的建立及初步应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年02期
页码:
357-362
栏目:
畜牧兽医·水产养殖
出版日期:
2019-04-30

文章信息/Info

Title:
Establishment and preliminary application of RT-PCR detection method for atypical porcine pestivirus
作者:
郭佳琪1; 杨晓宇1; 冯宇1; 杨钒1; 徐志文1; 2; 朱玲1; 2
(1.四川农业大学动物医学院，四川成都611130；2.四川农业大学动物疫病与人类健康四川省重点实验室，四川成都611130）
Author(s):
GUO Jia-qi1; YANG Xiao-yu1; FENG Yu1; YANG Fan1; XU Zhi-wen1; 2; ZHU Ling1; 2
(1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;2.Sichuan Provincial Key Laboratory of Animal Diseases and Human Health, Sichuan Agricultural University, Chengdu 611130, China)
关键词:
猪非典型性瘟病毒; RT-PCR; 检测
Keywords:
atypical porcine pestivirus (APPV); RT-PCR; detection
分类号:
S858.281.34+7
DOI:
doi:10.3969/j.issn.1000-4440.2019.02.016
文献标志码:
A
摘要:
为建立一种快速、准确检测猪非典型性瘟病毒（APPV）的方法，根据GenBank发布的APPV NS2基因序列，设计合成1对扩增后目的基因片段大小为500 bp的特异性引物。建立的RT-PCR方法对猪繁殖与呼吸综合征病毒（PRRSV）、伪狂犬病毒（PRV）和猪瘟病毒（CSFV）的检测结果均为阴性，该方法对APPV的最低检出量为1 μl4.95×104拷贝，重复性试验中组间、组内试验结果均与预期相符。应用该方法对68份疑似患病的仔猪样品进行检测，阳性病料检出率为7.35%。利用Mega7.0绘制APPV系统进化树，对APPV进行遗传进化分析，结果显示本试验检出的APPV（SC株）与中国报道的APPV的亲缘关系较近。建立的APPV RT-PCR检测方法可用于APPV的临床诊断及流行病学检测。
Abstract:
In order to establish a rapid and accurate method for detecting atypical porcine pestivirus (APPV), a pair of specific primers was designed and synthesized based on the APPV NS2 gene sequence published by GenBank, and the size of the target gene fragment was 500 bp after amplification. The detection results by the established RT-PCR method were negative for porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV) and swine fever virus (CSFV), and the minimum detectable amount of APPV was 4.95×104 copies per microlitre, and the results of inter group and intra group test in the repeated test were all in conformity with the expected results. The method was applied to detect 68 suspected cases of piglets, and the positive rate was 7.35%. Mega7.0 was used to draw the phylogenetic tree of APPV and analyze the genetic evolution of APPV. The results showed that the APPV detected in this test was closely related to the Chinese reported APPV. The RT-PCR detection method of APPV can be used for the clinical diagnosis and epidemiological detection of APPV

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-05-15 基金项目：四川省科技支撑计划项目（2017NZ0038）；“十二五”农村领域国家科技计划课题（2015BAD12B04-2.3）；“十三五”育种攻关计划项目（2016NYZ0052） 作者简介：郭佳琪（1996-），女，山东苍山人,本科。(E-mail)1511701068@qq.com 通讯作者：徐志文，(E-mail)abtcxzw@126.com
更新日期/Last Update: 2019-05-05