[1]赵为民,方晓敏,涂枫,等.猪单核源性巨噬细胞受FSL-1刺激后lncRNAs的鉴定与特征分析[J].江苏农业学报,2019,(02):346-356.[doi:doi:10.3969/j.issn.1000-4440.2019.02.015]
　ZHAO Wei-min,FANG Xiao-min,TU Feng,et al.Identification and characterization of lncRNA in porcine monocyte-derived macrophage stimulated by FSL-1[J].,2019,(02):346-356.[doi:doi:10.3969/j.issn.1000-4440.2019.02.015]
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猪单核源性巨噬细胞受FSL-1刺激后lncRNAs的鉴定与特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年02期
页码:
346-356
栏目:
畜牧兽医·水产养殖
出版日期:
2019-04-30

文章信息/Info

Title:
Identification and characterization of lncRNA in porcine monocyte-derived macrophage stimulated by FSL-1
作者:
赵为民1; 2; 方晓敏1; 2; 涂枫1; 2; 周李生1; 2; 李碧侠1; 2; 王学敏1; 2; 付言峰1; 2; 任守文1; 2
（1.江苏省农业科学院畜牧研究所/江苏省农业种质资源保护与利用平台，江苏南京210014；2.农业部种养结合重点实验室，江苏南京210014）
Author(s):
ZHAO Wei-min1; 2; FANG Xiao-min1; 2; TU Feng1; 2; ZHOU Li-sheng1; 2; LI Bi-xia1; 2; WANG Xue-min1; 2; FU Yan-feng1; 2; REN Shou-wen1; 2
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, China;2.Key Laboratory of Crop and Livestock Integration， Ministry of Agriculture, Nanjing 210014,China)
关键词:
猪; lncRNA; 单核源性巨噬细胞; FSL-1; 炎症
Keywords:
pig; lncRNA; monocyte-derived macrophage; FSL-1; inflammation
分类号:
S828
DOI:
doi:10.3969/j.issn.1000-4440.2019.02.015
文献标志码:
A
摘要:
lncRNA是新近发现的一种具有重要功能的RNA分子，在各种生理活动中发挥着重要作用。为鉴定猪单核源性巨噬细胞受FSL-1刺激后相关的lncRNA，利用二代测序技术结合生物信息学对lncRNA进行了组装与特征分析。结果显示FSL-1刺激成功地构建了细胞炎症模型，试验组与对照组共鉴定到1 056个lncRNA转录本，其对应于831个基因座，这些lncRNA的平均长度为1 643 bp，平均外显子数为2.4个。相对于对照组，试验组有51个lncRNA上调表达，44个lncRNA下调表达。在上调lncRNA两侧相邻的蛋白质编码基因参与免疫反应、炎症反应、病毒反应等通路，而在下调lncRNA两侧相邻的蛋白质编码基因参与的通路较少，不参与上述通路。定量PCR结果显示挑选的4个lncRNA的差异倍数与RNA-Seq的结果相似，其中上调的lncRNA呈现一定的组织特异表达，而下调的lncRNA呈现多组织表达。这些结果为进一步研究lncRNA在FSL-1介导的炎症反应中的作用奠定了基础。
Abstract:
LncRNA is a newly discovered RNA molecule with important functions and plays an important role in various physiological activities. To identify lncRNA in porcine monocyte-derived macrophage stimulated by FSL-1, lncRNA was assembled and characterized using next generation sequencing technology combined with bioinformatics. The results showed that the model of cell inflammation was successfully constructed with FSL-1 stimulation. A total of 1 056 lncRNA transcripts were identified in the experimental and control groups, which corresponded to 831 gene loci. The average length of these lncRNA was 1 643 bp, and these lncRNA span 2.4 exon on average. Compared to the control group, 51 and 44 lncRNAs were up-regulated and down-regulated in the experimental group, respectively. Protein encoding genes adjacent to both sides of up-regulated lncRNA participated in immune response, inflammatory response, and viral response pathways, while protein encoding genes adjacent to both sides of down-regulated lncRNAs participated in fewer pathways and did not participate in the above pathway. Quantitative PCR results showed that the differential folds of the four selected lncRNAs were similar to those of RNA-Seq, in which the up-regulated lncRNAs exhibited tissue-specific expression, while the down-regulated lncRNAs exhibited multi-tissue expression. These results lay the foundation for further study of the role of lncRNA in FSL-1 mediated inflammatory response.

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备注/Memo

备注/Memo:
收稿日期：2018-06-25 基金项目：国家自然科学基金项目（31601928）；国家生猪现代产业技术体系南京综合试验站(CARS-35)；江苏省农业科技自主创新基金项目[CX(17)2002] 作者简介：赵为民（1983-），男，湖北钟祥人，博士，副研究员，研究方向为猪的抗病育种。（Tel）025-84391941；（E-mail）zhao_weimin1983@aliyun.com 通讯作者：任守文，（Tel）025-84390349；（E-mail）shouwenren@163.com
更新日期/Last Update: 2019-05-05