[1]张斐然,冯静,张华峰,等.菊花中硒定量分析方法的建立与应用[J].江苏农业学报,2018,(04):927-932.[doi:doi:10.3969/j.issn.1000-4440.2018.04.030]
　ZHANG Fei-ran,FENG Jing,ZHANG Hua-feng,et al.Establishment and application of quantitative analysis method for selenium in Chrysanthemum morifolium[J].,2018,(04):927-932.[doi:doi:10.3969/j.issn.1000-4440.2018.04.030]
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菊花中硒定量分析方法的建立与应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
927-932
栏目:
加工贮藏·质量安全
出版日期:
2018-08-25

文章信息/Info

Title:
Establishment and application of quantitative analysis method for selenium in Chrysanthemum morifolium
作者:
张斐然1; 冯静1; 张华峰1; 赵爱青1; 薛梦莹1; 杨晓华2
（1.陕西师范大学食品工程与营养科学学院/中俄食品与健康科学国际联合研究中心/西北濒危药材资源开发国家工程实验室,陕西西安710119；2.西安交通大学医学部，卫生部法医学重点实验室,陕西西安710061）
Author(s):
ZHANG Fei-ran1; FENG Jing1; ZHANG Hua-feng1; ZHAO Ai-qing1; XUE Meng-ying1; YANG Xiao-hua2
(1.College of Food Engineering and Nutritional Science, Shaanxi Normal University, International Joint Research Center of Shaanxi Province for Food and Health Sciences, National Engineering Laboratory for Resources Development of Endangered Crude Drugs in
关键词:
硒; 菊花; 氢化物原子荧光光谱法; 定量分析
Keywords:
selenium; Chrysanthemum morifolium; hydride generation atomic fluorescence spectrometry; quantitative analysis
分类号:
TS207.3
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.030
文献标志码:
A
摘要:
为运用氢化物原子荧光光谱法测定陕西省市售菊花样品的硒含量。采用单因素试验和正交试验优化得到氢化物原子荧光光谱法的最佳测定条件为：负高压为320 V，灯电流为85 mA，硼氢化钠质量分数为1.0%。系统适应性试验结果显示，该方法的标准曲线方程为Y=116.86x+88.69（r=0.999 8），线性范围为 10.00～100.00 μg/L，检出限（LOD）、定量限（LOQ）分别为2.60 μg/L、7.88 μg/L，精密度为1.27%，平均回收率为98.38%。该方法精确、灵敏、简便、安全，分析成本较低。测定发现，陕西省各地市售菊花样品中硒的含量存在较大差异。
Abstract:
A hydride generation atomic fluorescence spectrometric method wasused to detect the content of selenium in Chrysanthemum morifolium samples purchased from Shaanxi province. The optimal conditions for the hydride generation atomic fluorescence spectrometry obtained by single factor experiments and orthogonal test were as follows: negative high voltage of 320 V, lamp current of 85 mA, and NaBH4 mass fraction of 1.0%. System suitability test results indicated that regression equation of standard curve was Y=116.86x+88.69, the correlation coefficient (r) was 0.999 8, and linear range was 10.00-100.00 μg/L. Limits of detection (LOD) and limits of quantitation (LOQ) were 2.60 μg/L and 7.88 μg/L, respectively. Precision and average recovery were 1.27% and 98.38%, respectively. The method is accurate, sensitive, simple, safe as well as low-cost. A large variation of selenium contents was found among Chrysanthemum morifolium samples from different regions in Shaanxi province.

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备注/Memo

备注/Memo:
收稿日期：2018-01-10 基金项目：陕西省重点研发计划项目（2018ZDXM-SF-006）；陕西省国际科技合作基地项目（2017GHJD-006）；陕西省安康市汉阴县人民政府科技创新项目（1204070141）；中央高校基本科研业务费专项资金项目（GK201703076、1301031567） 作者简介：张斐然(1994-)，女，山西榆次人，硕士研究生，研究方向为药食同源植物资源开发与利用。 通讯作者：张华峰， (Tel)029-85310517；(E-mail)isaacsau@sohu.com
更新日期/Last Update: 2018-09-04