[1]李素梅,施卫明.RNAi沉默水稻铵转运体OsAMT1表达的生长效应[J].江苏农业学报,2017,(06):1201-1206.[doi:doi:10.3969/j.issn.1000-4440.2017.06.001]
　LI Su-mei,SHI Wei-ming.The growth effect of rice by RNAi silencing ammonium transporter OsAMT1[J].,2017,(06):1201-1206.[doi:doi:10.3969/j.issn.1000-4440.2017.06.001]
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RNAi沉默水稻铵转运体OsAMT1表达的生长效应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1201-1206
栏目:
遗传育种·生理生化
出版日期:
2017-12-30

文章信息/Info

Title:
The growth effect of rice by RNAi silencing ammonium transporter OsAMT1
作者:
李素梅1; 施卫明2
（1.南京理工大学化工学院，江苏 南京210094；2.土壤与农业可持续发展国家重点实验室，中国科学院南京土壤研究所，江苏 南京 210008）
Author(s):
LI Su-mei1; SHI Wei-ming2
(1.School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China;2.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China)
关键词:
OsAMT1; RNAi; 表达水平; 生物量; 氮累积量
Keywords:
OsAMT1; RNAi; expression level; biomass; nitrogen accumulation
分类号:
Q786；Q945.12
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.001
文献标志码:
A
摘要:
OsAMT1是水稻中负责铵态氮高亲和吸收的基因家族，包括OsAMT1;1～OsAMT1;3 3个基因，已有的研究对其基因表达特征以及调控进行了大量的分析。OsAMT1;1表达水平最高，OsAMT1;2受氮水平诱导最为显著。因此，本研究选取OsAMT1序列上高度同源的区域，利用反向遗传学的RNAi技术构建再生OsAMT1家族3个基因共干扰沉默的转基因植株，分析OsAMT1基因表达水平降低引起的植株生长和氮累积量的变化。结果表明，依据OsAMT1同源序列构建的转基因水稻幼苗在低氮条件下基因的表达水平下降了 28%～60%，幼苗生长受到明显的抑制，氮累积量也受到显著抑制，说明OsAMT1在水稻的高亲和吸收铵态氮中占有重要地位。
Abstract:
OsAMT1 is a gene family responsible for high affinity ammonium uptake of rice, including OsAMT1;1, OsAMT1;2 and OsAMT1;3, which has been extensively studied for its function, expression and regulation. OsAMT1;1 was the biggest abundance gene in OsAMT1 family and OsAMT1; 2 was the most significant induction gene to nitrogen starvation. Therefore, in this study, we selected the homology sequence of OsAMT1 to construct the co-interfered transgenic plants through reverse genetics RNAi technology, subsequently analyzed the growth of transgenic plants caused by gene silencing. The results showed that the expression level of transgenic rice seedlings was decreased by 28%-60% under low nitrogen condition, the growth of seedlings was inhibited by OsAMT1 co-interfering, and the nitrogen accumulation was significantly inhibited, which indicated that OsAMT1 played an important role in rice high-affinity ammonium uptake.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-05-22 基金项目：国家自然科学青年基金项目（30800702） 作者简介：李素梅（1978-），女，安徽合肥人，博士，研究方向为氮素吸收转运机制。（E-mail）smli321@163.com
更新日期/Last Update: 2018-01-03