[1]曲绍轩,骆昕,徐平,等.腐食酪螨对阿维菌素的抗性相关基因鉴定与功能分析[J].江苏农业学报,2022,38(03):617-624.[doi:doi:10.3969/j.issn.1000-4440.2022.03.006]
　QU Shao-xuan,LUO Xin,XU Ping,et al.Identification and functional analysis of resistance genes of Tyrophagus putrescentiae Schrank to abamectin[J].,2022,38(03):617-624.[doi:doi:10.3969/j.issn.1000-4440.2022.03.006]
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腐食酪螨对阿维菌素的抗性相关基因鉴定与功能分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
617-624
栏目:
植物保护
出版日期:
2022-06-30

文章信息/Info

Title:
Identification and functional analysis of resistance genes of Tyrophagus putrescentiae Schrank to abamectin
作者:
曲绍轩; 骆昕; 徐平; 侯子强; 李辉平; 林金盛; 侯立娟; 蒋宁; 马林
（江苏省农业科学院蔬菜研究所，江苏南京210014）
Author(s):
QU Shao-xuan; LUO Xin; XU Ping; HOU Zi-qiang; LI Hui-ping; LIN Jin-sheng; HOU Li-juan; JIANG Ning; MA Lin
(Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
食用菌害螨; 抗性相关基因; RNAi; 抗药性
Keywords:
mushroom mite pest; resistance-related gene; RNAi; insecticide resistance
分类号:
S436.46+2
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.006
文献标志码:
A
摘要:
为揭示腐食酪螨对阿维菌素的抗性形成机制，通过RNA-seq技术获得亚致死剂量阿维菌素胁迫下腐食酪螨转录组序列，鉴定到138个差异表达的基因被注释到解毒代谢中，包括4个谷胱甘肽S转移酶基因（GST），12个细胞色素P450基因（P450）和3个羧酸酯酶基因（CarE）。利用RNAi技术沉默差异表达的11个解毒代谢基因后，腐食酪螨对阿维菌素的敏感性发生显著性变化，毒力指数在0.65～6.05。结果显示，GST家族中TPGST7、TPGST45和TPGST49以及P450基因TPCYP3A9、TPCYP3A13和TPCYP4C3均参与腐食酪螨对阿维菌素的抗性形成。此外，葡萄糖醛酸转移酶（GLT）家族基因TPGLT28首次被证实参与腐食酪螨对阿维菌素的抗性形成。
Abstract:
To reveal the farmation mechanism of the resistance of Tyrophagus putrescentiae to abamectin, the transcriptome sequence of Tyrophagus putrescentiae under a sublethal dose of abamectin was obtained by RNA-seq technology. A total of 138 differentially expressed genes were identified and annotated to detoxification metabolism, including four glutathione S transferase genes (GST), 12 cytochrome P450 genes (P450) and three carboxylesterase genes (CarE). After silencing the 11 differentially expressed genes by using RNAi technology, the susceptibility of the transcriptome sequence of Tyrophagus putrescentiae to abamectin changed significantly, and the virulence index ranged from 0.65 to 6.05. The results showed that TPGST7, TPGST45 and TPGST49 in GST family and P450 genes TPCYP3A9, TPCYP3A13 and TPCYP4C3 were involved in the formation of resistance of T. putrescentiae to abamectin. In addition, the glucuronosyltransferase (GLT) family gene TPGLT28 was first confirmed to be involved in the formation of abamectin resistance.

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备注/Memo

备注/Memo:
收稿日期：2021-11-16基金项目：财政部和农业农村部国家现代农业产业技术体系项目（CARS-20）；国家自然科学青年基金项目（32001911）；江苏省农业科技自主创新基金项目[CX(21)2021]作者简介：曲绍轩（1982-），女，辽宁大连人，博士，副研究员，主要从事食用菌有害生物防治研究。（Tel）025-84390159；（E-mail）qusx@jaas.ac.cn通讯作者：马林，（E-mail）malin1590@sina.com
更新日期/Last Update: 2022-07-07