[1]姜素平,张健,秦盛,等.抗茄链格孢菌放线菌的筛选及鉴定[J].江苏农业学报,2017,(03):543-549.[doi:doi:10.3969/j.issn.1000-4440.2017.03.009]
　JIANG Su-ping,ZHANG Jian,QIN Sheng,et al.Screening and identification of antagonistic actinomyces against Alternaria solani[J].,2017,(03):543-549.[doi:doi:10.3969/j.issn.1000-4440.2017.03.009]
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抗茄链格孢菌放线菌的筛选及鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年03期
页码:
543-549
栏目:
植物保护
出版日期:
2017-06-30

文章信息/Info

Title:
Screening and identification of antagonistic actinomyces against Alternaria solani
作者:
姜素平; 张健; 秦盛; 蒋继宏; 鞠秀云
（江苏师范大学生命科学学院/江苏省药用植物生物技术重点实验室，江苏徐州221116）
Author(s):
JIANG Su-ping; ZHANG Jian; QIN Sheng; JIANG Ji-hong; JU Xiu-yun
(Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou 221116, China)
关键词:
茄链格孢菌; 放线菌; 生防菌
Keywords:
Alternaria solani; actinomyces; biological control microorganism
分类号:
S482.2+92
DOI:
doi:10.3969/j.issn.1000-4440.2017.03.009
文献标志码:
A
摘要:
以茄链格孢菌为靶标菌，采用平板对峙法和生长速率法对分离自药用植物的221株放线菌进行筛选，通过生理生化特征、培养特征、形态特征和基于 16S rRNA基因序列的相似性分析对活性菌株的种属进行了鉴定。结果表明,抑菌率75%以上放线菌有3株，其中菌株3150被鉴定为环圈链霉菌（Streptomyces anulatus）的一个菌株，菌株 3679被鉴定为氢化链霉菌（Streptomyces hydrogenans）的一个菌株，菌株4536被鉴定为链霉菌属（Streptomyces sp.）菌株。薄层色谱分析结果显示菌株3679代谢产物丰富且分离度好，进一步分离可望得到具有较好生防作用的化合物。
Abstract:
To identify new bio-control agents against Alternaria solani, 221 strains of actinomyces from different medicinal plants were screened by plate confrontation method and growth rate method. The active strains were identified according to the physiological, cultural and morphological characteristics and 16S rRNA gene sequence analysis. There were 3 strains with inhibition rate against A. solani more than 75%, among which strain 3150 was identified as Streptomyces anulatus, strain 3679 was identified as S. hydrogenans, and strain 4536 was identified as the genus Streptomyces. Thin layer chromatography showed that strain 3679 was rich in metabolites and high in resolution. The compounds with bio-control effect was expected after further separation.

参考文献/References:

[1]唐岚,江厚利,王义勋,等. 链格孢属真菌分类研究进展[J]. 湖北林业科技, 2013, 42(4): 47-49.
[2]常缨,王义权,强胜. 链格孢菌菌株致病性及其遗传差异性[J]. 应用与环境生物学报, 2005, 11(4): 486-489.
[3]吴颖，侯潞丹，张杰．8种菌株代谢物对茄链格孢菌菌丝生长及孢子萌发的抑制［J］．江苏农业学报，2016,32(2):293-298.
[4]卜元卿,孔源,智勇,等. 化学农药对环境的污染及其防控对策建议[J]. 中国农业科技导报, 2014, 16(2): 19-25.
[5]DEMAIN A L, SANCHEZ S. Microbial drug discovery: 80 years of progress[J]. Antibiot, 2009, 62: 5-16.
[6]MERCADO-FLORES Y, CRDENAS-LVAREZ I O, ROJAS-OIVERA A V, et al. Application of Bacillus subtilis in the biological control of the phytopathogenic fungus Sporisorium reilianum[J]. Biological Control, 2014, 76: 15-22.
[7]TORRES M J, PEREZ B C, PETROSELLI G, et al. Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of Fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds[J]. Microbiological Research, 2016, 182: 31-39.
[8]YAN L L, HAN N N, ZHANG Y Q, et al. Antimycin A 18 produced by an endophytic Streptomyces albidoflavus isolated from a mangrove plant [J]. The Journal of Antibiotics, 2010, 63: 259-261.
[9]USAMA R A, TANJA G, SRIKKANTH B, et al. Elicitation of secondary metabolism in actinomycetes[J]. Biotechnology Advances, 2015, 33: 798-811.
[10]张丽,纪明山,于志国. 娄彻氏链霉菌 YL-2 代谢产物对稻瘟病菌的抑制性及其稳定性[J]. 沈阳农业大学学报, 2014, 45(2): 143-146.
[11]张清明,王彩霞,王海艳,等. 苹果树腐烂病内生拮抗放线菌A-2的鉴定及其活性评价[J]. 农药学学报, 2013, 15(3): 286-292.
[12]中国科学院微生物研究所放线菌分类组. 链霉菌鉴定手册[M]. 北京: 科学出版社, 1975:13-15, 658-661.
[13]韩长志. 植物病害生防菌的研究现状及发展趋势[J]. 中国森林病虫, 2015, 34(1): 33-38.
[14]JONATHAN A C, JONATHAN D R, LOUISE R, et al. A simple inhibition coefficient for quantifying potency of biocontrol agents against plant-pathogenic fungi[J]. Biological Control, 2015, 81: 93-100.
[15]张斌,乔俊卿,梁雪杰,等. 番茄枯萎病菌和青枯病菌拮抗细菌的评价[J]. 植物保护学报, 2015, 42(3): 353-361.
[16]陈思宇,陈志谊,张荣胜. 水稻纹枯病菌拮抗细菌的筛选及鉴定[J]. 植物保护学报, 2013, 40(3): 211-218.
[17]XUE L, XUE Q H, ChEN Q, et al. Isolation and evaluation of rhizosphere actinomycetes with potential application for biocontrol of Verticillium wilt of cotton[J]. Crop Protection, 2013, 43: 231-240.
[18]邱德文. 生物农药的发展现状与趋势分析[J]. 中国生物防治学报, 2015, 31(5): 679-684.
[19]COUILLEROT O, LOQMAN S, TORIBIO A, et al. Purification of antibiotics from the biocontrol agent Streptomyces anulatus S37 by centrifugal partition chromatography [J]. Joumal of Chromatography B, 2014, 944: 30-34.
[20]SALEH O, FLINSPACH K, WESTRICH L, et al. Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663 [J]. Beilstein Journal Organic Chemistry, 2012, 8(1): 501-513.
[21]胡磊,牛世全,景彩虹,等. 拮抗油菜菌核病菌的链霉菌分离筛选与鉴定[J]. 中国油料作物学报, 2013, 35(1): 69-73.
[22]秦盛,赵立兴,陈云,等.药用植物内生放线菌的分离、筛选及活性菌株 YIM 61470 鉴定[J]. 微生物学通报, 2009, 36(11): 1693-1699.

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备注/Memo

备注/Memo:
收稿日期：2016-12-02 基金项目：江苏师范大学一般项目（2015YYB132） 作者简介：姜素平（1989-），女，山东菏泽人，硕士研究生，研究方向为微生物代谢产物，(E-mail)supingjiang123@163.com 通讯作者：鞠秀云，(E-mail)6020070004@jsnu.edu.cn
更新日期/Last Update: 2017-06-29