[1]李辉,果双双,孟春花,等.猪髓样细胞触发因子1CDR区的克隆、表达及生物活性[J].江苏农业学报,2016,(05):1100-1106.[doi:10.3969/j.issn.1000-4440.2016.05.023]
　LI Hui,GUO Shuang-shuang,MENG Chun-hua,et al.Cloning and expression of the CDR area from swine triggering receptor expressed on myeloid cells 1 and its bioactivity[J].,2016,(05):1100-1106.[doi:10.3969/j.issn.1000-4440.2016.05.023]
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猪髓样细胞触发因子1CDR区的克隆、表达及生物活性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
1100-1106
栏目:
畜牧兽医·水产养殖
出版日期:
2016-11-22

文章信息/Info

Title:
Cloning and expression of the CDR area from swine triggering receptor expressed on myeloid cells 1 and its bioactivity
作者:
李辉1; 果双双2; 孟春花1; 周亚文3; 施振旦1
1. 江苏省农业科学院畜牧研究所，江苏南京210014；2. 山东农业大学动物科技学院，山东泰安271018；3. 青岛职业技术学院,山东青岛266555
Author(s):
LI Hui1; GUO Shuang-shuang2; MENG Chun-hua1; ZHOU Ya-wen3; SHI Zhen-dan1
1. Institute of Animal Science， Jiangsu Academy of Agricultural Sciences, Nanjing 210014，China；2. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018，China；3. Qingdao Polytechnical College, Qingdao 266555，China
关键词:
猪; 髓样细胞触发因子1; 肺泡巨噬细胞; 促炎症因子
Keywords:
swine; triggering receptor expressed on myeloid cells; alveolar macrophage; proinflammatory cytokine
分类号:
S828
DOI:
10.3969/j.issn.1000-4440.2016.05.023
文献标志码:
A
摘要:
本研究旨在探讨重组猪TREM-1基因CDR区蛋白对猪肺泡巨噬细胞受到脂多糖（LPS）刺激后炎症因子表达状态的改变。首先克隆猪TREM-1基因CDR区，然后构建重组载体并转入BL21(DE3)中，IPTG诱导表达、纯化并复性猪TREM-1基因CDR蛋白。将不同浓度复性的重组蛋白添加到1 000 ng/ml LPS处理的猪肺泡巨噬细胞培养液中，然后通过荧光定量PCR方法分别测定主要促炎症因子（IL-1β、IL-2、IL-4、IL-6、IL-8、IL-10、IL-12、IL-16、IL-18及TNFα）的表达规律。结果显示，重组猪TREM-1CDR区蛋白可显著降低由LPS诱导的主要促炎症因子的表达。表明重组猪TREM-1基因CDR区蛋白具有抑制炎症反应的生物学活性。
Abstract:
In the present study, the changes of proinflammatory cytokines expression were investigated in lipopolysaccharides (LPS)-stimulated porcine alveolar macrophages by recombinant TREM-1 CDR protein. Swine TREM-1 CDR area was cloned and inserted into prokaryotic expression vector. After IPTG induction of BL21（DE3）competent cell, the recombinant swine TREM-1 CDR protein was purified. Serial concentrations of recombinant protein were supplemented into the culture medium of 1 000 ng/ml LPS treated porcine alveolar macrophages. The expressions of proinflammatory cytokines （IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-16, IL-18 and TNFα）were analyzed using QRT-PCR. Results showed that proinflammatory cytokines were down-regulated by the recombinant swine TREM-1 CDR protein at the concentration of 1 000 ng/ml. The results suggest that recombinant swine TREM-1 CDR protein is bioactive in inhibiting inflammatory response.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2016-01-08 基金项目：国家自然科学基金项目（31402080）；江苏省自然科学基金项目（BK20151365） 作者简介：李辉（1982-），男，山东惠民人，博士，副研究员，主要从事动物繁殖研究。（Tel）025-84390772；(E-mail)lhlydk@126.com。 通讯作者：施振旦，(E-mail)zdshi@scau.edu.cn
更新日期/Last Update: 2016-11-22