[1]丛汉卿,齐尧尧,朱文丽,等.木薯CKX基因的序列分析及其乙烯和茉莉酸甲酯诱导表达特性[J].江苏农业学报,2016,(03):506.[doi:10.3969/j.issn.1000-4440.2016.03.004]
　CONG Han-qing,QI Yao-yao,Zhu Wen-li,et al.Sequence analysis of CKX gene and expression characteristics induced by ethylene and methyl jasmonate in Manihot esculenta Crantz[J].,2016,(03):506.[doi:10.3969/j.issn.1000-4440.2016.03.004]
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木薯CKX基因的序列分析及其乙烯和茉莉酸甲酯诱导表达特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
506
栏目:
遗传育种·生理生化
出版日期:
2016-06-30

文章信息/Info

Title:
Sequence analysis of CKX gene and expression characteristics induced by ethylene and methyl jasmonate in Manihot esculenta Crantz
作者:
丛汉卿;  齐尧尧;  朱文丽;  陈松笔;  李 丽
中国热带农业科学院热带作物品种资源研究所/农业部热带作物种质资源利用重点开放实验室,海南 儋州 571737
Author(s):
CONG Han-qing;  QI Yao-yao;  Zhu Wen-li;  CHEN Song-bi;  LI Li
Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crops Germplasm Resources Utilization, Ministry of Agriculture, Danzhou 571737, China
关键词:
木薯;  CKX基因;  乙烯;  茉莉酸;  表达分析
Keywords:
cassava;  CKX gene;  ethylene;  jasmonate;  expression analysis
分类号:
S533
DOI:
10.3969/j.issn.1000-4440.2016.03.004
文献标志码:
A
摘要:
木薯是一种重要的能源和淀粉作物,具有较强的抗逆性。为阐明逆境信号调控细胞分裂素代谢关键基因CKX的分子机制,对乙烯利和茉莉酸甲酯处理后的木薯品种华南八号叶片数字基因表达谱中两个表达差异显著的MeCKX基因进行了序列分析,发现两者具有乙烯响应元件,但没有茉莉酸响应元件。以华南八号悬浮培养细胞为材料,利用qRT-PCR检测了木薯MeCKX1和MeCKX2基因在乙烯利和茉莉酸甲酯处理后的表达特性。结果显示:茉莉酸信号可使这两个基因显著上调; 乙烯信号可使MeCKX1表达量缓慢上调,使MeCKX2表达量缓慢下调。说明这两个MeCKX基因可被乙烯和茉莉酸信号调控,并推测一方面其基因启动子区相应的特异响应元件参与了调控,另一方面两种逆境信号物质分别通过影响其他调控途径,间接造成这两个基因的不同表达,以进一步影响细胞分裂素的代谢。
Abstract:
Cassava(Manihot esculenta Crantz)is an important energy and starch crop,with strong stress resistance. In an attempt to illustrate the molecular mechanism of CKX, a key gene for cytokinin metabolism stimulated by stress signals in cassava,two differentially expressed MeCKX gene sequences were screened from Digital Gene Expression Profiling data with cassava SC8 leaves exposed to ethephon and methyl jasmonate.Sequence analysis shows that two MeCKXs have ethylene responsive elements, but no MeJA responsive elements in promoter regions. Expression patterns of MeCKX1 and MeCKX2 identified by quantitative real time PCR technique revealed that the two genes were up-regulated significantly after treated with methyl jasmonate. Ethylene treatment increased the MeCKX1 expression and decreased the MeCKX2 expression slowly. The results suggest that two MeCKX genes could be regulated by ethylene and jasmine acid signals. It can also be inferred that some specific responsive elements in promoter regions might have participated in the expression regulation. Meanwhile, the two stress signals might have played roles in the metabolism of cytokinin by affecting other regulation pathways, resulting in differential expressions of two MeCKXs.

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备注/Memo

备注/Memo:
收稿日期:2015-10-11
基金项目:中央级公益性科研院所基本科研业务费专项(1630032014036)
作者简介::丛汉卿(1983-),男,山东潍坊人,博士,助理研究员,主要从事分子生物学和生物信息学研究。(Tel)13698948460;(E-mail)hiigara@yeah.net
通讯作者:李 丽,(E-mail)chenli907@163.com
更新日期/Last Update: 2016-06-30