[1]姬改革,胡艳,陶志云,等.蛋内注射T3 对鸭胚骨骼肌发育和MSTN mRNA 表达的影响[J].江苏农业学报,2016,(01):164-169.[doi:10.3969/j.issn.1000-4440.2016.01.025 ]
 JI Gai-ge,HU Yan,TAO Zhi-yun,et al.Effects of in ovo injecting triiodothyronine into egg on embryonic skeletal muscle development and expression of myostatin mRNA in duck[J].,2016,(01):164-169.[doi:10.3969/j.issn.1000-4440.2016.01.025 ]
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蛋内注射T3 对鸭胚骨骼肌发育和MSTN mRNA 表达的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年01期
页码:
164-169
栏目:
畜牧兽医·水产养殖
出版日期:
2016-01-08

文章信息/Info

Title:
Effects of in ovo injecting triiodothyronine into egg on embryonic skeletal muscle development and expression of myostatin mRNA in duck
作者:
姬改革胡艳陶志云刘宏祥朱春红李慧芳
(江苏省家禽科学研究所,江苏扬州225003)
Author(s):
JI Gai-geHU YanTAO Zhi-yunLIU Hong-xiangZHU Chun-hongLI Hui-fang
(Jiangsu Institute of Poultry Science, Yangzhou 225003, China)
关键词:
蛋内注射T3鸭胚骨骼肌MSTN
Keywords:
in ovo injectiontriiodothyronineduck embryoskeletal musclemyostatin
分类号:
S834.8
DOI:
10.3969/j.issn.1000-4440.2016.01.025
文献标志码:
A
摘要:
为探讨生长因子三碘甲状腺原氨酸(Triiodothyronine,T3)对鸭胚骨骼肌生长发育和肌肉生长抑制素(Myostatin,MSTN) mRNA表达的影响,在入孵前的鸭种蛋蛋清内分别注射100 μl的生理盐水溶液(对照组)和含0.25 μg T3的生理盐水溶液(注射组),检测13、17、21、25、27胚龄鸭胸肌和腿肌发育及骨骼肌MSTN mRNA的表达情况。结果显示,T3处理极显著提高27胚龄鸭胚的胸肌质量,但对腿肌质量影响不显著;21胚龄注射组胸肌MSTN mRNA表达显著高于对照组,27胚龄则显著低于对照组;注射组腿肌MSTN mRNA表达仅在25胚龄显著增加。相关性分析结果显示,两组骨骼肌质量与MSTN mRNA表达呈不同程度的线性相关。表明蛋内注射T3能增加鸭胚胸肌质量,并改变MSTN mRNA表达量,MSTN mRNA的差异表达可能在鸭胚胸肌发育过程中具有重要的作用。
Abstract:
In order to investigate the effect of triiodothyronine (T3) on duck embryonic skeletal muscle development and the expression of myostatin(MSTN) mRNA, the 100 μl saline solution (control group) and T3 saline solution containing 0.25 μg T3 (injection group) were in ovo injected into duck eggs before hatching. The skeletal muscle development on embryonic days 13, 17, 21, 25 and 27 were measured and the expression of MSTN mRNA were detected using real-time fluorescence quantitative PCR. The results showed that in ovo injection of T3 significantly increased the breast muscle weight in embryonic age 27, but had no significant influence on embryonic leg muscle weight. The expression of MSTN mRNA in injection group was significantly higher at embryonic age 21 than that in control group but lower at embryonic age 27. The MSTN mRNA expression of leg muscle in injection group only increased significantly in embryonic age 25. There were liner correlations between skeletal muscles weights and MSTN mRNA expression. It was concluded that in ovo injection of T3 increased the duck embryonic breast muscle weight, along with the changes of MSTN mRNA expression level, and differential expression of MSTN mRNA may play an important role in embryonic breast muscle development.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-05-29 基金项目:国家自然科学基金项目(31172194);江苏省科技支撑计划项目(BE2014362);江苏省农业科技自主创新基金项目[CX(14)2079] 作者简介:姬改革(1985-),女,河南洛阳人,硕士,助理研究员,主要从事家禽遗传育种与资源保护研究。(E-mail)jigaige@126.com 通讯作者:李慧芳,(E-mail)lhfxf_002@aliyun.com
更新日期/Last Update: 2017-05-08