[1]吴健,朱海霞,赵志荀,等.山羊痘病毒Ａ１１Ｒ 基因的克隆及生物信息学分析[J].江苏农业学报,2016,(01):158-163.[doi:10.3969/j.issn.1000-4440.2016.01.024 ]
　WU Jian,ZHU Hai-xia,ZHAO Zhi-xun,et al.Cloning and bioinformatics analysis of A11R gene of Goatpox virus[J].,2016,(01):158-163.[doi:10.3969/j.issn.1000-4440.2016.01.024 ]
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山羊痘病毒Ａ１１Ｒ 基因的克隆及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年01期
页码:
158-163
栏目:
畜牧兽医·水产养殖
出版日期:
2016-01-08

文章信息/Info

Title:
Cloning and bioinformatics analysis of A11R gene of Goatpox virus
作者:
吴健12; 朱海霞1; 赵志荀1; 颜新敏1; 赵银龙3; 吴娜1; 李健1; 张志东1; 张强1; 李影2; 吴国华1
（1.中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/农业部畜禽病毒学重点开放实验室，甘肃兰州730046；2.吉林农业大学动物科学技术学院，吉林长春130118；3.西北民族大学生命科学技术学院，甘肃兰州730124）
Author(s):
WU Jian12; ZHU Hai-xia1; ZHAO Zhi-xun1; YAN Xin-min1; ZHAO Yin-long3; WU Na1; LI Jian1; ZHANG Zhi-dong1; ZHANG Qiang1; LI Ying2; WU Guo-hua
(1.State Key Laboratory of Veterinary Etiological Biology /Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China；2.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;3.Academy of Life Science and Technology, Northwest University for Nationalities, Lanzhou 730124, China)
关键词:
羊痘病毒; A11R; 基因克隆; 生物信息学
Keywords:
Capripox virus; A11R; gene clone; bioinformatics
分类号:
S827
DOI:
10.3969/j.issn.1000-4440.2016.01.024
文献标志码:
A
摘要:
为了分析绵羊痘病毒A11R蛋白质的分子特征，提取了山羊痘病毒古浪分离株(GL)的基因组DNA，设计引物进行PCR扩增，将PCR产物连接到pGEM-T Easy载体后转化至大肠杆菌DH5α感受态细胞，筛选阳性克隆进行序列测定，利用生物信息学软件对A11R基因序列进行预测分析。结果显示，A11R基因序列由954个核苷酸组成的开放阅读框，编码317个氨基酸残基组成的多肽，蛋白质分子质量的理论值为 36 091.7，理论等电点为5.14。A11R蛋白质的二级结构中，α螺旋占29.02%，β折叠占10.41%，其余60.57%为无规则卷曲。多序列比对分析显示，不同羊痘病毒分离株A11R序列高度保守，同源性在98%以上。进化树分析结果显示,GL株与NK株、TU株以及SA株在一个分支,说明它们之间具有较近的亲缘关系，上述研究结果为进一步研究A11R蛋白质的生物学功能和羊痘病毒早期蛋白质分子间相互作用奠定了基础。
Abstract:
To explore the molecular characteristics of A11R from Goatpox virus(GTPV), genomic DNA was extracted from Goatpox virus GuLang strains. The specific primers were designed and used to amplify A11R gene from genomic DNA by PCR. The PCR product was ligated into pGEM-T Easy vector. After transformation into Escherichia coli DH5α, the positive clones were sequenced and the sequences were analyzed with the bioinformatic softwares. The result showed A11R gene sequence contained an open reading frame(ORF) of 954 nucleotides and the deduced protein consisted of 317 amino acids with the theoretical molecular weight of 36 091.7 and isoelectric point of 5.14. Analysis of secondary structure of protein A11R revealed that α-helix, β-strand and loop accounted for 29.02%, 10.41% and 60.57%, respectively. Multiple sequence alignment showed A11R gene from different Capripoxvirus isolates were highly conserved with similarity as high as 98％. Phylogenetic analysis demonstrated that, GL and NK, TU and SA were grouped in one branch, indicative a close genetic relationship.

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备注/Memo

备注/Memo:
收稿日期：2015-05-13 基金项目：国家质检总局公益性行业科研专项（201310093）；国家自然科学基金项目（31201892）；甘肃省自然科学基金项目（1208RJZA101）；国家“863”计划项目（2012AA101304） 作者简介：吴健（1990-），男，吉林长春人，硕士研究生，主要从事分子免疫学研究。（Tel）13019125792;（E-mail）358496480@qq.com 通讯作者：吴国华，（Tel）18919924902；（E-mail） wuguohua@caas.cn；李影，（E-mail） thliying@163.com
更新日期/Last Update: 2017-05-08