[1]张欣,张小丽,马小军,等.甘肃高山细毛羊TLR2基因遗传多态性及其与乳房炎相关性[J].江苏农业学报,2015,(06):1357-1361.[doi:doi:10.3969/j.issn.1000-4440.2015.06.025]
　ZHANG Xin,ZHANG Xiao-li,MA Xiao-jun,et al.Genetic polymorphism of TLR2 gene and its correlation with mastitis in Gansu fine-wool sheep[J].,2015,(06):1357-1361.[doi:doi:10.3969/j.issn.1000-4440.2015.06.025]
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甘肃高山细毛羊TLR2基因遗传多态性及其与乳房炎相关性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1357-1361
栏目:
畜牧兽医·水产养殖
出版日期:
2015-12-31

文章信息/Info

Title:
Genetic polymorphism of TLR2 gene and its correlation with mastitis in Gansu fine-wool sheep
作者:
张欣1; 张小丽1; 马小军1; 李发弟23; 张晨1; 陈富强1; 宋晓育1
(1.甘肃农业大学动物医学院，甘肃兰州730070；2.甘肃农业大学动物科学技术学院，甘肃兰州730070；3.甘肃省肉羊繁育生物技术工程实验室,甘肃民勤733300）
Author(s):
ZHANG Xin1; ZHANG Xiao-li1; MA Xiao-jun1; LI Fa-di23; ZHANG Chen1; CHEN Fu-qiang1; SONG Xiao-yu1
(1.College of Veterinary Medicine,Gansu Agricultural University, Lanzhou 730070, China;2.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;3.Mutton Sheep Breeding Biotechnology Engineering Laboratory of Gansu province, Minqin 733300, China)
关键词:
甘肃高山细毛羊; TLR2基因; 乳房炎; PCR-SSCP; 体细胞评分 
Keywords:
Gansu alpine fine-wool sheep; TLR2 gene; mastitis; PCR-SSCP; somatic cell score
分类号:
S821.2
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.025
文献标志码:
A
摘要:
以286只甘肃高山细毛羊为研究对象，利用PCR-SSCP方法结合测序的方法进行TLR2基因多态位点检测，并通过构建一般线性模型分析其多态性与体细胞评分(SCS)的相关性，以期为甘肃高山细毛羊乳房炎抗性选育提供参考。结果显示，试验群体发现2种等位基因控制的3种基因型(AA、AB、BB)，其中B等位基因为优势等位基因，等位基因频率为0.71，而A等位基因频率则为0.29。经χ2适合性检验，突变达到Hardy-weinberg平衡状态(P>0.05)。测序结果与GenBank 中绵羊TLR2基因序列比对显示，扩增片段分有5处核苷酸多态位点，并导致了3处发生氨基酸改变。AA型个体的SCS均值显著低于AB、BB型（P<0.05），A等位基因基因型可作为乳房炎抗性的优良基因。因此，可将TLR2基因作为甘肃高山细毛羊乳房炎抗感性候选基因应用于乳房炎抗性筛选。
Abstract:
To improve mastitis resistance breeding in Gansu alpine fine-wool sheep, the polymorphic loci of 〖WTBX〗TLR2 gene in 286 Gansu fine-wool sheep were detected by PCR-SSCP and sequencing, and the correlation between polymorphism and somatic cell score (SCS) was analyzed. The results showed that two alleles, A and B, controlled three genotypes AA, AB, and BB. Allele B was dominant allele, with the allele frequency of 0.71. Chi-square test revealed the mutations reached Hardy-Weinberg equilibrium(P>0.05). Five polymorphism loci were detected in 〖WTBX〗TLR2 gene, leading to amino acid changes in three sites. The somatic cell score(SCS) of AA was significantly lower than those of AB and BB (P<0.05), indicating allele A could be used as a dominant genotype for mastitis resistance. In conclusion, 〖WTBX〗TLR2 could be regarded as candidate gene of Gansu alpine fine-wool sheep for mastitis resistance selection.

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备注/Memo

备注/Memo:
收稿日期：2015-03-19 基金项目：甘肃省农业生物技术研究与应用开发项目（GNSW-2011-22）;甘肃省高等学校基本科研业务费项目 作者简介：张欣（1988-），女，山东诸城人，硕士研究生，研究方向为动物免疫与抗病。（E-mail）zhangxinfreedom@163.com 通讯作者：张小丽,（E-mail）zhxl228@163.com
更新日期/Last Update: 2015-12-31