[1]陈泽斌,李冰,王定康,等.Illumina MiSeq高通量测序分析核桃内生细菌多样性[J].江苏农业学报,2015,(05):1129-1133.[doi:doi:10.3969/j.issn.1000-4440.2015.05.029]
　CHEN Ze-bin,LI Bing,WANG Ding-kang,et al.Diversity of endophytic bacteria in walnut analyzed by Illumina MiSeq high-throughput sequencing technology[J].,2015,(05):1129-1133.[doi:doi:10.3969/j.issn.1000-4440.2015.05.029]
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Illumina MiSeq高通量测序分析核桃内生细菌多样性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1129-1133
栏目:
园艺
出版日期:
2015-10-31

文章信息/Info

Title:
Diversity of endophytic bacteria in walnut analyzed by Illumina MiSeq high-throughput sequencing technology
作者:
陈泽斌12; 李冰3; 王定康4; 余磊1; 徐胜光1; 任禛1; 靳松1; 张永福1; 彭声静1
（1.昆明学院农学院，云南昆明650214；2.云南省都市特色农业工程技术研究中心，云南昆明650214；3.中国科学院昆明动物研究所，云南昆明650223；4.昆明学院生命科学与技术系，云南昆明650214）
Author(s):
CHEN Ze-bin12; LI Bing3; WANG Ding-kang4; YU Lei1; XU Sheng-guang1; REN Zhen1; JIN Song1; ZHANG Yong-fu1; PENG Sheng-jing1
(1.College of Agriculture, Kunming University, Kunming 650214, China;2.Engineering Research Center for Characteristic Agriculture of Yunnan Province, Kunming 650214, China;3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China;4.Department of Life Science and Technology, Kunming University, Kunming 650214, China)
关键词:
Illumina MiSeq; 高通量测序; 核桃; 内生细菌; 多样性
Keywords:
high-throughput sequencing; walnut; endophytic bacterium; diversity
分类号:
S664.143.6
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.029
文献标志码:
A
摘要:
应用Illumina MiSeq高通量测序技术测定核桃内生细菌的16S rDNA-V4变异区序列，使用Uparse等软件整理和统计样品序列数目和操作分类单元（OTUs）数量，分析内生细菌的丰度和α-多样性。获得了用于分析的有效序列63 183条，OTU数为103。稀释曲线表明测序深度充分，OTU数量接近于饱和。核桃样品的Chao1指数为105.143，Shannon多样性指数为1.823。核桃内生细菌主要分布于鞘氨醇单胞菌属（Sphingomonas，27.27%）、盐单胞菌属（Halomonas，27.27%）、土壤杆菌属（Agrobacterium，45.45%），这3个属是核桃内生细菌优势菌属。
Abstract:
In this study, the species abundance and alpha diversity of walnut endophytic bacteria were analyzed by Illumina MiSeq high-throughput sequencing of the 16S rDNA-V4 region. Softwares such as Uparse, Flash, and Qiime were employed to sort and calculate the number of sequences and operational taxonomic units (OTUs). The numbers of effective sequences and OTUs for each sample were 63183 and 103, respectively. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. Majority of the endophytic bacteria belonged to Sphingomonas(27.27%), Halomonas(27.27%) and Agrobacterium(45.45%) which were therefore the dominant bacterial families in walnut. Illumina MiSeq high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria.

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备注/Memo

备注/Memo:
收稿日期：2015-04-23 基金项目：国家自然科学基金项目（41361056）；云南省教育厅科学研究项目（2014Y390）；云南省都市型休闲农业工程技术研究中心项目（XXNY1402）；昆明学院大学生科学研究项目（XJD15054）；云南省高校优势特色重点学科（生态学）建设项目 作者简介：陈泽斌（1985-），男，云南昆明人，博士，副教授，主要从事环境微生物的教学及科研工作。（Tel）18687102882；（E-mail）zbchenkmu@163.com 通讯作者：李冰,（Tel）18987895038；（E-mail）libing@mail.kiz.ac.cn
更新日期/Last Update: 2015-10-31