[1]彭玉娇,崔学宇,邵元元,等.不同树龄沙田柚果园土壤肥力、叶片养分和土壤细菌群落的特征[J].江苏农业学报,2021,(02):348-354.[doi:doi:10.3969/j.issn.1000-4440.2021.02.010]
　PENG Yu-jiao,CUI Xue-yu,SHAO Yuan-yuan,et al.Characteristic of soil fertility, leaf mineral nutrients and bacterial community in Shatian pomelo orchards of different tree ages[J].,2021,(02):348-354.[doi:doi:10.3969/j.issn.1000-4440.2021.02.010]
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不同树龄沙田柚果园土壤肥力、叶片养分和土壤细菌群落的特征()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
348-354
栏目:
耕作栽培·资源环境
出版日期:
2021-04-30

文章信息/Info

Title:
Characteristic of soil fertility, leaf mineral nutrients and bacterial community in Shatian pomelo orchards of different tree ages
作者:
彭玉娇1; 崔学宇1; 邵元元1; 杨艳丽1; 杜潇1; 贾书刚1; 刘书田1; 区燕丽2
（1.南宁师范大学/广西地标作物大数据工程技术研究中心/北部湾环境演变与资源利用教育部重点实验室/广西地表过程与智能模拟重点实验室，广西南宁530001;2.容县土肥植保经作站,广西容县537500）
Author(s):
PENG Yu-jiao1; CUI Xue-yu1; SHAO Yuan-yuan1; YANG Yan-li1; DU Xiao1; JIA Shu-gang1; LIU Shu-tian1; OU Yan-li2
（1.Guangxi Geographical Indication Crops Research Center of Big Data Mining and Experimental Engineering Technology/Key Laboratory of Environment Change and Resources Utilization of Beibu Gulf, Ministry of Education/Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Nanning Normal University, Nanning 530001, China;2.Soil Fertilizer, Plant Protection and Economic Crops Station of Rong County, Rong County 537500, China）
关键词:
沙田柚; 树龄; 土壤; 叶片营养; 高通量测序
Keywords:
Citrus grandis var. shatinyu; tree-age; soil; leaf nutrient; high throughput sequencing
分类号:
S666.3
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.010
文献标志码:
A
摘要:
通过分析不同树龄沙田柚果园土壤肥力和果树叶片营养成分的变化，以期为沙田柚果园精准施肥管理提供理论依据。选择广西壮族自治区玉林市容县12个不同树龄的沙田柚果园，对沙田柚果园土壤肥力指标及果树叶片营养成分含量进行测定，利用高通量测序技术对土壤细菌群落进行测序。结果表明，12个沙田柚果园土壤呈酸性，pH值、有机质含量、土壤全氮含量随着沙田柚树龄的增加而增加，不同树龄沙田柚果园土壤之间的土壤有效镁含量、有效硫含量和有效硼含量没有显著差异，树龄≥21年的沙田柚果园土壤全钾含量和交换性钙含量最高；不同树龄沙田柚果树叶片氮含量无显著差异，5～10年树龄沙田柚果树叶片磷含量、钾含量、硫含量最高，≥21年树龄沙田柚果树叶片钙含量最高，11～15年树龄沙田柚果树叶片镁含量和硼含量最高。共获得15 903个操作分类单元（OTUs），沙田柚果园土壤中细菌至少涵盖31个门、42个纲、101个目、180个科、347个属、222个种。在门水平上分析可知，变形菌门、放线菌门和绿弯菌门是优势菌门，随着沙田柚栽培时间的增加，放线菌门的相对丰度持续提高，酸杆菌门、疣微菌门的相对丰度持续降低。沙田柚果园土壤肥力随树龄变化明显，树龄、土壤因子改变了沙田柚栽培果园土壤中细菌的群落结构。
Abstract:
The changes of soil fertility, leaf mineral nutrients contents in orchards of Shatian pomelo with different tree ages were analyzed to provide theoretical basis for accurate fertilizing management in orchards of Shatian pomelo. 12 Shatian pomelo orchards with different tree ages were selected in Rong County of Yuling City in Guangxi Zhuang Autonomous Region to determine the soil fertility indices and leaf nutrients contents in orchards of Shatian pomelo. Meanwhile, high-throughput sequencing technique was adopted to sequence the soil bacterial community. The results showed that, soils of 12 orchards were acidic, pH value, organic matter content and soil total N content increased with the increasing of tree ages. There were no significant differences in available Mg content, available S content and available B content between soils of Shatian pomelo orchards with different tree ages. The contents of total K and interchangeable Ca were the highest in orchards with the tree age of more than 21 years. There was no significant difference in leaf N content between Shatian pomelo trees with different ages. The contents of P, K and S in leaves of Shatian pomelo were the highest in the group with the tree ages of five to ten years, the content of Ca in leaves of Shatian pomelo was the highest in the group with more than 21 years tree age, and the contents of Mg and B in leaves of Shatian pomelo were the highest in the group with 11-15 years tree age.A total of 15 903 operational taxonomic units (OTUs) were obtained in this study, including at least 31 phyla, 42 classes, 101 orders, 180 families, 347 genera and 222 species of bacteria in soils of Shatian pomelo orchards. Proteobacteria, Actinobacteria and Chloroflexi were the dominant phyla at the phylum level. With the increase of cultivation time of Shatian pomelo, the relative abundance of Actinobacteria increased persistently, while the relative abundance of Acidobacteria and Verrucomicrobia continued to decrease. The soil fertility of Shatian pomelo orchards changed with the tree ages obviously. Therefore, the bacterial community structure of Shatian pomelo orchards was changed by tree ages and soil factors.

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备注/Memo

备注/Memo:
收稿日期：2020-08-14基金项目：广西科技重大专项(桂科AA17204077)；广西一流学科（地理学）建设项目；北部湾环境演变与资源利用教育部重点实验室系统基金项目（GTEU-KLOP-X1820、GTEU-KLOP-X1819）作者简介：彭玉娇（1991-）,女，吉林松原人，硕士，助理研究员，主要从事沙田柚品质研究。(E-mail)1356346392@qq.com通讯作者：崔学宇，(E-mail)yaoyuan200452@163.com
更新日期/Last Update: 2021-05-10