[1]李汉全,张炳火,杨建远,等.Streptomyces eurocidicus JXJ 0089对铜绿微囊藻的抑制[J].江苏农业学报,2015,(05):1037-1044.[doi:doi:10.3969/j.issn.1000-4440.2015.05.015]
　LI Han-quan,ZHANG Bing-huo,YANG Jian-yuan,et al.Cyanobacteria-lytic activity of Streptomyces eurocidicus JXJ 0089 against Microcystis aeruginosa[J].,2015,(05):1037-1044.[doi:doi:10.3969/j.issn.1000-4440.2015.05.015]
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Streptomyces eurocidicus JXJ 0089对铜绿微囊藻的抑制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1037-1044
栏目:
耕作栽培·资源环境
出版日期:
2015-10-31

文章信息/Info

Title:
Cyanobacteria-lytic activity of Streptomyces eurocidicus JXJ 0089 against Microcystis aeruginosa
作者:
李汉全; 张炳火; 杨建远; 查代明; 过七根; 石翔; 郭静
（九江学院药学与生命科学学院，江西九江332000）
Author(s):
LI Han-quan; ZHANG Bing-huo; YANG Jian-yuan; ZHA Dai-ming; GUO Qi-gen; SHI Xiang; GUO Jing
(College of Pharmacy and Life Science, Jiujiang University, Jiujiang 332000, China)
关键词:
铜绿微囊藻; Streptomyces eurocidicus; 抑制活性; 附生细菌
Keywords:
Microcystis aeruginosa; Streptomyces eurocidicus; inhibitory activity; attached bacterium
分类号:
X172
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.015
文献标志码:
A
摘要:
采用藻平板法，筛选到1株对铜绿微囊藻具有抑制活性的放线菌。根据形态鉴定和16S rRNA基因序列分析，鉴定为Streptomyces eurocidicus JXJ 0089。研究了该放线菌培养时间、活性成分的极性、孢子或菌丝体与无细菌藻或有细菌藻的相互影响、附生细菌对藻细胞和放线菌的影响。结果表明，S. eurocidicus JXJ 0089培养11 d后，抑藻活性较强，活性成分含有多种极性不同的物质；放线菌孢子对藻细胞没有抑制作用，但菌丝体显著抑制藻细胞生长，且对无细菌藻的抑制活性显著高于有细菌藻；藻细胞亦抑制放线菌，尤其是抑制放线菌孢子萌发；附生细菌对铜绿微囊藻生长有促进作用，而对放线菌有抑制作用，这是放线菌菌丝体对有细菌藻的抑制活性低于无细菌藻的重要原因，也是放线菌的孢子和菌丝体在有细菌藻液中死亡的主要原因。
Abstract:
An actinomycete strain, with inhibition activity on Microcystis aeruginosa, was screened out using algae lawn. Based on the morphology and 16S rRNA gene sequence analysis, the strain was identified as Streptomyces eurocidicus JXJ 0089. After incubated for 11 d, the culture broth of S. eurocidicus JXJ 0089 exhibited strong inhibitory activity on M. aeruginosa, and the active components contained metabolites different polarities. It was not the spores of S. eurocidicus JXJ 0089 but the mycelia yielded inhibitory activity on M. aeruginosa. and the activity was stronger on pure culture of M. aeruginosa than on the impure. M. aeruginosa inhibited S. eurocidicus JXJ 0089 as well, especially the germination of the spores. Attached bacteria of M. aeruginosa promoted the growth of M. aeruginosa, but inhibited the growth of S. eurocidicus JXJ 0089, which might be responsible for the higher inhibitory effect on pure M. aeruginosa and the death of spores and mycelia of S. eurocidicus JXJ 0089 in the culture of impure M. aeruginosa. 

参考文献/References:

[1]CHEN W M, SHEU F S, SHEU S Y. Novel L-amino acid oxidase with algicidal activity against toxic cyanobacterium Microcystis aeruginosa synthesized by a bacterium Aquimarina sp. [J]. Enzyme and Microbial Technology, 2011, 49(4): 372-379.
[2]DAGNINO D, DE ABREU MEIRELES D, DE AQUINO ALMEIDA J C. Growth of nutrient-replete Microcystis PCC 7806 cultures is inhibited by an extracellular signal produced by chlorotic cultures [J]. Environmental Microbiology, 2006, 8(1): 30-36.
[3]GUO L. Doing battle with the green monster of Taihu Lake [J]. Science, 2007, 317(5842): 1166.
[4]WU X G, JOYCE E M, MASON T J. The effects of ultrasound on cyanobacteria [J]. Harmful Algae, 2011, 10(6): 738-743.
[5]AZEVEDO S M, CARMICHAEL W W, JOCHIMSEN E M, et al. Human intoxication by microcystins during renal dialysis treatment in Caruaru-Brazil [J]. Toxicology, 2002, 181-182: 441-446.
[6]MIZUNO C S, SCHRADER K K, RIMANDO A M. Algicidal activity of stilbene analogues [J]. Journal of Agricultural and Food Chemistry, 2008, 56(19): 9140-9145.
[7]SCHRADER K K, NANAYAKKARA N P, TUCKER C S, et al. Novel derivatives of 9, 10-anthraquinone are selective algicides against the musty-odor cyanobacterium Oscillatoria perornata [J]. Applied and Environmental Microbiology, 2003, 69(9): 5319-5327.
[8]DAVIS T W, BERRY D L, BOYER G L， et al. The effects of temperature and nutrients on the growth a dynamics of toxic and non-toxic strains of Microcystis during cyanobacterial blooms [J]. Harmful Algae, 2009, 8(5): 715-725.
[9]JAN〖KG-*4〗C〖DD(-*2〗ˇ〖DD)〗ULA D, DRBKOVM, C〖DD(-*2〗ˇ〖DD)〗ERN〖KG-*4〗Y〖DD(-*2〗｀〖DD)〗 J, et al. Algicidal activity of phthalocyanines-screening of 31 compounds [J]. Environmental Toxicology, 2008, 23(2): 218-223.
[10]刘恩生. 生物操纵与非经典生物操纵的应用分析及对策探讨[J]. 湖泊科学, 2010, 22(3): 307-314.
[11]PAN G, ZHANG M M, CHEN H, et al. Removal of cyanobacterial blooms in Taihu Lake using local soils. I. Equilibrium and kinetic screening on the flocculation of Microcystis aeruginosa using commercially available clays and minerals [J]. Environmental Pollution, 2006, 141(2): 195-200.
[12]XU Y F, YANG J, WANG Y L, et al. The effects of jet cavitation on the growth of Microcystis Aeruginosa [J]. Journal of Environmental Science and Health, Part A, 2006, 41(10): 2345-2358.
[13]YAN R, WU Y H, JI H L, et al. The decoction of Radix astragali inhibits the growth of Microcystis aeruginosa [J]. Ecotoxicology and Environmental Safety, 2011, 74(4): 1006-1010.
[14]YAMAMOTO Y, KOUCHIWA T， HODOKI Y. Distribution and identification of actinomycetes lysing cyanobacteria in a eutrotrophic lake [J]. Journal of Applied Phycology, 1998, 10(4): 391-397.
[15]KUMAR H D. Streptomycin and penicillin-induced inhibition of growth and pigment production in blue-green algae and production of strains of Anacystis nidulana resistant to these antibiotics [J]. Journal of Experimental Botany, 1964, 15(2):232-250.
[16]QIAN H, LI J J, PAN X J, et al. Effects of streptomycin on growth of algae Chlorella vulgaris and Microcystis aeruginosa [J]. Environmental Toxicology, 2012, 27(4): 229-237.
[17]EI-SHERBINY S A, GHALY M F, EI-AYOTY Y M， et al. Niromycin A: an antialgal substance produced by Streptomyces endus N40 [J]. Research Journal of Microbiology, 2007, 2(8): 606-618.
[18]EI-SHERBINY S A, EI-AYOTY Y M, GHALY M F, et al. Evaluation for the production of antialgal substance from Streptomyces neyagawaensis [J]. Biotechnology, 2009, 8(4): 405-415.
[19]SCHRADER K K, RIMANDO A M, DUKE S O. Natural compounds for the management of undesirable freshwater phytoplankton blooms[J]. Studies in Natural Products Chemistry, 2002, 26: 351-389.
[20]FENG Y, CHANG X X, ZHAO L X, et al. Nanaomycin a methyl ester, an actinomycete metabolite: algicidal activity and the physiological response of Microcystis aeruginosa [J]. Ecological Engineering, 2013, 53: 306-312.
[21]LUO J F, WANG Y, TANG S S, et al. Isolation and identification of algicidal compound from Streptomyces and algicidal mechanism to Microcystis aeruginosa [J]. Plos One, 2013, 8(10): 1-14.
[22]HEHMANN A, KAYA K， WATANABE M M. Selective control of Microcystis using an amino acid——a laboratory assay [J]. Journal of Applied Phycology, 2002, 14(2): 85-89.
[23]PARK M H, CHUNG I M, AHMAD A, et al. Growth inhibition of unicellular and colonial Microcystis strains (Cyanophyceae) by compounds isolated from rice (Oryza sativa) hulls [J]. Aquatic Botany, 2009, 90(4): 309-314.
[24]GUMBO J R, CLOETE T E, VAN ZYL G J J, et al. The viability assessment of Microcystis aeruginosa cells after co-culturing with Bacillus mycoides B16 using flow cytometry [J]. Physics and Chemistry of the Earth, 2014, 72-75: 24-33.
[25]DAWSON R M. The toxicology of microcystins [J]. Toxicon, 1998, 36(7): 953-962.
[26]余甜甜,张炳火,李汉全,等. 一株放线菌对铜绿微囊藻的抑藻活性 [J]. 生态与农村环境学报, 2011, 27(2): 58-63.
[27]徐丽华,李文均,刘志恒,等. 放线菌系统学-原理、方法及实践 [M]. 北京：科学出版社, 2007.
[28]陈宇炜,陈开宁,胡耀辉. 浮游植物叶绿素a测定的“热乙醇法”及其测定误差的探讨 [J]. 湖泊科学, 2006, 18(5): 550-552.
[29]张炳火,张玉琴,李汉全,等. 链霉菌JXJ-402产生的抗生素JXJ-402-1的研究 [J]. 中国抗生素杂志, 2007, 32(5): 280-281, 299.
[30]HATANO K, NISHII T, KASAI H. Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. Rev [J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(5): 1519-1529.
[31]SAFFERMAN R S, MORRIS M. Evaluation of natural products for algicidal properties [J]. Applied Microbiology, 1962, 10: 289-292.
[32]WHYTE L G, MAULE A, CULLIMORE D. Method for isolating cyanobacterial-lysing Streptomycetes from soil [J]. J Appl Bacteriol, 1985, 58: 195-197.
[33]ZHANG B H, CHENG J, LI L, et al. Streptomyces jiujiangensis sp. nov., isolated from soil in South China [J]. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2014, 105(4): 763-770.
[34]ZHANG B H, CHENG J, CHEN W, et al. Streptomyces lushanensis sp. nov., a novel actinomycete with anti-cyanobacterial activity[J]. The Journal of Antibiotics, 2015, 68(1): 5-8.
[35]SIGEE D C, GLENN R, ANDREWS M J, et al. Biological control of cyanobacteria: principles and possibilities [J]. Hydrobiologia, 1999, 395-396: 161-172.
[36]CHOI H J, KIM B H, KIM J D, et al. Streptomyces neyagawaensis as a control for the hazardous biomass of Microcystis aeruginosa (Cyanobacteria) in eutrophic freshwaters [J]. Biological Control, 2005, 33(3): 335-343.
[37]OSATO T, UEDA M, FUKUYAMA S, et al. Production of tertiomycin (a new antibiotic substance), azomycin and eurocidin by S. eurocidicus [J]. The Journal of Antibiotics, 1955, 8(4): 105-109.
[38]TAKAMURA Y, YAMADA T, KIMOTO A, et al. Growth inhibition of Microcystis cyanobacteria by L-lysine and disappearance of natural Microcystis blooms with spraying [J]. Microbes and Environments, 2004, 19(1): 31-39.
[39]杨柳燕,肖琳. 湖泊蓝藻水华爆发、危害与控制 [M]. 北京: 科学出版社，2011.
[40]董娟,何晓红,李大平,等. 太湖梅梁湾附生细菌和游离细菌群落结构分析 [J]. 应用与环境生物学报, 2011, 17(1): 104-108.
[41]SHI L M, CAI Y F, KONG F X, et al. Specific association between bacteria and buoyant Microcystis colonies compared with other bulk bacterial communities in the eutrophic Lake Taihu, China[J]. Environmental Microbiology Reports, 2012, 4(6): 669-678.
[42]SHI L M, CAI Y F, YANG H L, et al. Phylogenetic diversity and specificity of bacteria associated with Microcystis aeruginosa and other cyanobacteria [J]. Journal of Environmental Sciences, 2009, 21(11): 1581-1590.
[43]DZIALLAS C， GROSSART H P. Temperature and biotic factors influence bacterial communities associated with the cyanobacterium Microcystis sp [J]. Environmental Microbiology, 2011, 13(3): 1632-1641.
[44]PARVEEN B, RAVET V, DJEDIAT C, et al. Bacterial communities associated with Microcystis colonies differ from free-living communities living in the same ecosystem [J]. Environmental Microbiology Reports, 2013, 5(5): 716-724.〖ZK）〗〖FL）〗

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备注/Memo

备注/Memo:
收稿日期：2015-03-30 基金项目：国家自然科学基金项目（31060010）；江西省科技计划项目（20111BBG70012-4）；江西省环保厅科技项目（JXHBKJ2013-14）；江西省教育厅科技项目（GJJ10619） 作者简介：李汉全（1962-），男，江西九江人，本科，副教授，主要研究方向为微生物资源。（E-mail）lihanquan62@126.com 通讯作者：张炳火，（E-mail）binghuozh@126.com
更新日期/Last Update: 2015-10-31