[1]林婷婷,孙芝兰,刘芳,等.微酸性电解水对产气荚膜梭菌芽孢的杀灭效果及作用机制[J].江苏农业学报,2023,(08):1755-1761.[doi:doi:10.3969/j.issn.1000-4440.2023.08.016]
　LIN Ting-ting,SUN Zhi-lan,LIU Fang,et al.Bactericidal effect and mechanism of action of slightly acidic electrolyzed water on Clostridium perfringens spores[J].,2023,(08):1755-1761.[doi:doi:10.3969/j.issn.1000-4440.2023.08.016]
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微酸性电解水对产气荚膜梭菌芽孢的杀灭效果及作用机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年08期
页码:
1755-1761
栏目:
加工贮藏·质量安全
出版日期:
2023-12-31

文章信息/Info

Title:
Bactericidal effect and mechanism of action of slightly acidic electrolyzed water on Clostridium perfringens spores
作者:
林婷婷1; 2; 孙芝兰2; 刘芳2; 吴海虹1; 张春红1; 王道营1; 2
(1.沈阳农业大学食品学院,辽宁沈阳110866；2.江苏省农业科学院农产品加工研究所,江苏南京210014)
Author(s):
LIN Ting-ting1; 2; SUN Zhi-lan2; LIU Fang2; WU Hai-hong1; ZHANG Chun-hong1; WANG Dao-ying1; 2
(1.College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;2.Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
微酸性电解水; 产气荚膜梭菌; 芽孢
Keywords:
slightly acidic electrolyzed water; Clostridium perfringens; spores
分类号:
TS201.6
DOI:
doi:10.3969/j.issn.1000-4440.2023.08.016
文献标志码:
A
摘要:
微酸性电解水(SAEW)是一种安全有效的新型杀菌剂。本研究分析了SAEW对产气荚膜梭菌芽孢的杀灭效果,并研究了其杀灭芽孢的作用机制。结果显示,10 mg/L、20 mg/L和30 mg/L SAEW处理30 min后产气荚膜梭菌芽孢存活数明显降低,SAEW杀灭效果明显。并且40 mg/L SAEW处理5 min就可以完全灭活芽孢。扫描电子显微镜观察结果表明,质量浓度为40 mg/L SAEW处理可使芽孢干瘪皱缩,失去原本饱满的芽孢形态。激光共聚焦显微镜观察结果显示,与对照组相比,40 mg/L SAEW处理过的芽孢均呈现红色,说明芽孢的外膜被SAEW破坏,并且芽孢全部死亡。相同处理时间,随着SAEW质量浓度的不断提高,芽孢的疏水性不断降低, Zeta-电位绝对值和粒径不断变小,并且芽孢内核物质DPA大量释放,这说明SAEW处理破坏了芽孢的内膜结构。因此,SEAM可通过破坏芽孢的内膜结构及外层结构达到杀灭效果。
Abstract:
Slightly acidic electrolyzed water (SAEW) is a new safe and effective bactericide. In this study, the bactericidal effect of SAEW on Clostridium perfringens spores was analyzed, and the mechanism of its action in killing the spores was studied. The results showed that the survival number of Clostridium perfringens spores decreased significantly after 30 minutes of treatment with 10 mg/L, 20 mg/L, and 30 mg/L SAEW, indicating a significant bactericidal effect. Moreover, the C. perfringens spores were completely inactivated after treatment with 40 mg/L SAEW for 5 min. Scanning electron microscopy results showed that treatment with 40 mg/L SAEW could make the spores shrivel and shrink. Confocal laser scanning microscopy showed that all the SAEW-treated spores at 40 mg/L showed red color compared with the control group, indicating that the outer membrane of the spores was destroyed by SAEW, and all the spores died. With the increasing of SAEW concentration, the hydrophobicity of spores decreased, the absolute value of zeta-potential and particle size became smaller, and the spore core substance DPA was released more and more, which indicated that SAEW treatment disrupted the inner membrane structure of the spores. Therefore, SAEM can achieve bactericidal effect by disrupting the inner membrane structure and outer structure of spores.

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备注/Memo

备注/Memo:
收稿日期:2022-10-18基金项目:江苏省农业科技自主创新基金项目[CX(22)2044]作者简介:林婷婷(1996-),女,河南信阳人,硕士研究生,研究方向为食品微生物。(E-mail)Ltt9625@163.com通讯作者:吴海虹,(E-mail) 305087638@qq.com；张春红, (E-mail)zhangchsy@163.com
更新日期/Last Update: 2024-01-15