[1]王佳琦,王欣,邓小梅,等.花榈木生物碱合成关键酶基因OhpLDC和OhpCAO1的克隆与分析[J].江苏农业学报,2023,(08):1646-1657.[doi:doi:10.3969/j.issn.1000-4440.2023.08.004]
　WANG Jia-qi,WANG Xin,DENG Xiao-mei,et al.Cloning and analysis of key enzyme genes OhpLDC and OhpCAO1 for alkaloid synthesis in Ormosia henryi Prain[J].,2023,(08):1646-1657.[doi:doi:10.3969/j.issn.1000-4440.2023.08.004]
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花榈木生物碱合成关键酶基因OhpLDC和OhpCAO1的克隆与分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年08期
页码:
1646-1657
栏目:
遗传育种·生理生化
出版日期:
2023-12-31

文章信息/Info

Title:
Cloning and analysis of key enzyme genes OhpLDC and OhpCAO1 for alkaloid synthesis in Ormosia henryi Prain
作者:
王佳琦; 王欣; 邓小梅; 吴蔼民
(华南农业大学林学与风景园林学院,广东广州510000)
Author(s):
WANG Jia-qi; WANG Xin; DENG Xiao-mei; WU Ai-min
(College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510000, China)
关键词:
花榈木; 喹诺里西啶类生物碱; 赖氨酸脱羧酶; 铜胺氧化酶; 生物信息学
Keywords:
Ormosia henryi Prain; quinolizidine alkaloids; lysine decarboxylase; copper amine oxidase; bioinformatics
分类号:
S792.99
DOI:
doi:10.3969/j.issn.1000-4440.2023.08.004
文献标志码:
A
摘要:
花榈木是中国传统中药,其代谢产物常用于治疗跌打损伤,具有重要的药用价值,然而目前人们对花榈木的药用化学成分如生物碱合成并不清楚。本研究对花榈木不同组织进行了代谢组和转录组学分析,结果表明花榈木中含有的生物碱大部分属于喹诺里西啶类生物碱(Quinolizidine Alkaloids,QA)。进一步分析发现,QA生物合成的2个关键酶,赖氨酸脱羧酶(LDC) 和铜胺氧化酶(CAO) 在生物碱合成中起重要作用。结合花榈木转录组结果对其编码基因克隆,得到了1 290 bp、2 268 bp CDS序列,分别命名为OhpLDC和OhpCAO1。生物信息学分析结果表明,OhpLDC 和OhpCAO1编码的蛋白质相对分子质量分别为4.63×104和8.44×104,均无跨膜区域和信号肽。OhpLDC编码的氨基酸序列具有保守的底物结合位点Phe340,分析发现OhpLDC与豆科植物中的LDC基因在进化上具有较近的亲缘关系。OhpCAO1编码的氨基酸序列中具有保守结构域“NY-Y”,以及3个组氨酸保守位点,与狭叶羽扇豆的CAO1亲缘关系最近。本研究结果为解析花榈木QA的生物合成途径提供了重要基础。
Abstract:
Ormosia henryi Prain is a traditional Chinese medicine, and its metabolites are commonly used to treat injuries and have important medicinal value. However, the medicinal chemical components of Ormosia henryi Prain, such as alkaloid synthesis, are currently unclear. This study conducted metabolomic and transcriptomic analysis on different tissues of Ormosia henryi Prain, and the results showed that most of the alkaloids contained in Ormosia henryi Prain belonged to quinolizidine alkaloids (QA). Further analysis revealed that the two key enzymes involved in QA biosynthesis, lysine decarboxylase (LDC) and copper amine oxidase (CAO), played important roles in alkaloid synthesis. Based on the transcriptome results of Ormosia henryi Prain, the coding genes were cloned, and two CDS sequences of 1 290 bp and 2 268 bp were obtained, named OhpLDC and OhpCAO1 respectively. The bioinformatics analysis results indicated that the relative molecular weights of the proteins encoded by OhpLDC and OhpCAO1 were 4.63×104 and 8.44×104, and there were no transmembrane regions and signal peptides. The amino acid sequence encoded by OhpLDC had a conserved substrate binding site Phe340. Analysis revealed that OhpLDC had a close evolutionary relationship with LDC genes in leguminous plants. The amino acid sequence encoded by OhpCAO1 had a conserved domain "NY-Y" and three histidine conserved sites. The amino acid sequence encoded by OhpCAO1 had the closest genetic relationship with the CAO1 of narrow-leaved lupin. The results of this study provide an important basis for analyzing the biosynthetic pathway of QA in Ormosia henryi Prain.

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备注/Memo

备注/Memo:
收稿日期:2022-10-27基金项目:广东省林业科技创新项目(2017KJCX028)作者简介:王佳琦(1998-),女,黑龙江大庆人,硕士研究生,主要从事花榈木组学研究。(E-mail)wangjiaqi1990@163.com通讯作者:吴蔼民,(E-mail)wuaimin@scau.edu.cn
更新日期/Last Update: 2024-01-15