[1]赵胡,沈程,郝庆萍,等.香椿中3个水通道蛋白基因(TsAQP)的克隆和表达分析[J].江苏农业学报,2023,(08):1635-1645.[doi:doi:10.3969/j.issn.1000-4440.2023.08.003]
　ZHAO Hu,SHEN Cheng,HAO Qing-ping,et al.Cloning and expression analysis of three aquaporin genes (TsAQP) in Toona sinensis[J].,2023,(08):1635-1645.[doi:doi:10.3969/j.issn.1000-4440.2023.08.003]
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香椿中3个水通道蛋白基因(TsAQP)的克隆和表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年08期
页码:
1635-1645
栏目:
遗传育种·生理生化
出版日期:
2023-12-31

文章信息/Info

Title:
Cloning and expression analysis of three aquaporin genes (TsAQP) in Toona sinensis
作者:
赵胡1; 2; 沈程1; 2; 郝庆萍1; 2; 刘艳红3; 季春艳3; 石潇瀑1
(1.阜阳师范大学生物与食品工程学院,安徽阜阳236037；2.抗衰老中草药安徽省工程技术研究中心,安徽阜阳236037；3.阜阳师范大学信息工程学院,安徽阜阳236041)
Author(s):
ZHAO Hu1; 2; SHEN Cheng1; 2; HAO Qing-ping1; 2; LIU Yan-hong3; JI Chun-yan3; SHI Xiao-pu1
(1.Biology and Food Engineering College, Fuyang Normal University, Fuyang 236037, China；2.Engineering Technology Research Center of Anti-aging Chinese Herb, Fuyang 236037, China；3.College of Information Engineering, Fuyang Normal University, Fuyang 236041, China)
关键词:
香椿; 水通道蛋白; 基因克隆
Keywords:
Toona sinensis; aquaporin; gene cloning
分类号:
S644.4
DOI:
doi:10.3969/j.issn.1000-4440.2023.08.003
文献标志码:
A
摘要:
水通道蛋白(Aquaporins,AQP)能够促进水分和小分子物质在膜上的运输,维持细胞水分平衡。本研究利用香椿转录组数据,克隆到3个香椿水通道蛋白基因(TsAQP),即TsPIP1-5(GenBank登录号MT501152)、TsPIP2-5(GenBank登录号MT501153)和TsTIP2-1(GenBank登录号MT501154)。利用生物信息学和实时荧光定量PCR(qRT-PCR)技术,结合生理表型分析,研究TsPIP1-5、TsPIP2-5和TsTIP2-1蛋白性质、表达模式和在香椿芽采后贮藏中的功能。结果表明:从转录组数据中鉴定出的20个TsAQP可分为5类；TsPIP1-5、TsPIP2-5和TsTIP2-1是通过跨膜区定位于膜的疏水性稳定蛋白,含磷酸化位点,无信号肽；TsPIP1-5、TsPIP2-5和TsTIP2-1在根、茎和叶中均有表达且有一定的器官差异性；TsPIP1-5、TsPIP2-5和TsTIP2-1在香椿芽低温贮藏过程中表达量提高,暗示TsAQP在香椿芽采后脱水过程中有调控作用。本研究结果为进一步研究香椿中TsAQP基因的功能和作用机制奠定了基础。
Abstract:
Aquaporins (AQP) facilitate the transport of water and small molecules across membranes and maintain water balance of cells. Three aquaporin genes in Toona sinensis (TsAQP), TsPIP1-5(GenBank accession number MT501152), TsPIP2-5(GenBank accession number MT501153), and TsTIP2-1(GenBank accession number MT501154), were cloned based on transcriptome sequencing. Using a combination of bioinformatics and real-time fluorescence quantitative PCR (qRT-PCR), we studied the physiological phenotypes, protein properties, expression patterns, and function of TsPIP1-5, TsPIP2-5 and TsTIP2-1 during post harvest storage of Toona sinensis buds. The results indicated that 20 TsAQP proteins identified from the transcriptome data could be classified into five subfamilies. TsPIP1-5, TsPIP2-5 and TsTIP2-1 were hydrophobic stable proteins located on the membrane through the transmembrane region, containing phosphorylation sites and no signal peptide. Different expression levels of TsPIP1-5, TsPIP2-5, and TsTIP2-1 were detected in the roots, stems and leaves with certain organ differences. The expression levels of TsPIP1-5, TsPIP2-5, and TsTIP2-1 increased during cold storage of Toona sinensis buds, suggesting that they played a regulatory role in the process of post harvest dehydration. The results of this study lay a foundation for further research on function and regulatory mechanisms of TsAQPs in Toona sinensis.

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备注/Memo

备注/Memo:
收稿日期:2022-11-27基金项目:安徽省高等学校自然科学基金重点项目(KJ2021A0683)；阜阳师范大学-阜阳市2021年度市校合作科技专项(SXHZ202107)；2020年度高校优秀拔尖人才培育资助项目(gxgwfx2020049)；阜阳师范大学自然科学重点项目(2020FSKJ01ZD)作者简介:赵胡(1977-),男,安徽六安人,博士,副教授,主要从事植物分子生物学研究。(E-mail)zhaohu8196@sina.com通讯作者:石潇瀑,(E-mail)tiramisu.shi@163.com
更新日期/Last Update: 2024-01-15