[1]张家旭,王信,叶倩女,等.松针多酚提取工艺优化及其抗氧化、酶活性抑制特征[J].江苏农业学报,2023,(07):1593-1605.[doi:doi:10.3969/j.issn.1000-4440.2023.07.016]
　ZHANG Jia-xu,WANG Xin,YE Qian-nü,et al.Optimization of extraction process of polyphenols from pine needles and its antioxidant and enzyme activity inhibitory characteristics[J].,2023,(07):1593-1605.[doi:doi:10.3969/j.issn.1000-4440.2023.07.016]
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松针多酚提取工艺优化及其抗氧化、酶活性抑制特征()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年07期
页码:
1593-1605
栏目:
加工贮藏·质量安全
出版日期:
2023-10-31

文章信息/Info

Title:
Optimization of extraction process of polyphenols from pine needles and its antioxidant and enzyme activity inhibitory characteristics
作者:
张家旭1; 2; 王信1; 2; 叶倩女1; 2; 董学凤1; 2; 郭玉儿2; 3; 彭腾腾1; 2; 尹盼盼1; 2; 李海燕1; 2; 石晓峰1; 2; 3
(1.甘肃中医药大学药学院，甘肃兰州730030；2.甘肃省医学科学研究院，甘肃兰州730050；3.兰州理工大学，甘肃兰州730050)
Author(s):
ZHANG Jia-xu1; 2; WANG Xin1; 2; YE Qian-nü1; 2; DONG Xue-feng1; 2; GUO Yu-er2; 3; PENG Teng-teng1; 2; YIN Pan-pan1; 2; LI Hai-yan1; 2; SHI Xiao-feng1; 2; 3
(1.College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730030, China;2.Gansu Provincial Academic Institute for Medical Research, Lanzhou 730050, China; 3. Lanzhou University of Technology, Lanzhou 730050, China)
关键词:
松针; 多酚; 提取工艺; 抗氧化; 酶活性抑制
Keywords:
pine needles; polyphenols; extraction process; antioxidant; enzyme activity inhibition
分类号:
TS201.4
DOI:
doi:10.3969/j.issn.1000-4440.2023.07.016
文献标志码:
A
摘要:
为优化松针多酚的提取工艺，并考察其体外抗氧化作用以及对α-葡萄糖苷酶、α-淀粉酶、酪氨酸酶活性的抑制作用，本研究利用超声辅助提取法，在提取温度、提取时间、乙醇体积分数、液料比等要素对松针多酚提取单因素试验的基础上，利用响应面法优化松针多酚提取工艺；并利用优化工艺条件，测定了6种松科植物松针的多酚提取量。进一步利用不同多酚含量的松针提取液，分析松针多酚对1,1-二苯基-2-苦基肼自由基(DPPH)、2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸自由基(ABTS+)、羟基自由基(·OH)的清除能力及其对α-葡萄糖苷酶、α-淀粉酶、酪氨酸酶活性的抑制能力。结果表明，松针多酚的最佳提取工艺为提取温度60 ℃、提取时间28 min、乙醇体积分数60%、料液比1 g∶25 ml；在此条件下，6种松科植物松针多酚提取量为19.97～53.41 mg/g。6种松科植物松针多酚对DPPH自由基清除的IC50值范围为22.76～159.90 μg/ml，对ABTS+自由基清除的IC50值范围为0.092～0.184 mg/ml，对·OH自由基清除的IC50值范围为2.176～8.134 mg/ml，对α-葡萄糖苷酶活性的抑制IC50值范围为6.421～18.120 μg/ml，对α-淀粉酶活性的抑制IC50值范围为0.399～10.570 mg/ml，对酪氨酸酶活性的抑制IC50值范围为0.121～3.725 mg/ml。总之，松针具有较高的多酚含量，明显的抗氧化活性，对α-葡萄糖苷酶、α-淀粉酶、酪氨酸酶活性有较强的抑制作用，在天然抗氧化剂、降糖药的研发中有广泛的应用前景。
Abstract:
In order to optimize the extraction process of pine needle polyphenols, and to investigate its antioxidant effect in vitro and its inhibitory effect on the activities of α-glucosidase, α-amylase and tyrosinase, based on the single factor test of extraction temperature, extraction time, ethanol volume fraction and liquid-to-material ratio on the content of polyphenols in pine needle extract, the extraction process of pine needle polyphenols based on ultrasonic-assisted extraction was optimized by response surface method. The extraction of polyphenols from pine needles of six pine plants was determined by using the optimized process conditions. The scavenging ability of pine needle polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,2 ′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical (ABTS+) and hydroxyl radical (·OH) and their inhibitory ability on the activities of α-glucosidase, α-amylase and tyrosinase were further analyzed by using pine needle extracts with different polyphenol contents. The results showed that the optimum extraction conditions were as follows: extraction temperature 60 ℃, extraction time 28 min, ethanol volume fraction 60%, liquid-solid ratio 1∶25 (g∶ml). Under these conditions, the extraction amount of polyphenols from pine needles of six pine plants ranged from 19.97 mg/g to 53.41 mg/g. The IC50 values of DPPH free radical, ABTS+ free radical, ·OH free radical were 22.76-159.90 μg/ml, 0.092-0.184 mg/ml, 2.176-8.134 mg/ml, respectively, and the IC50 values of α-glucosidase, α-amylase and tyrosinase were 6.421-18.120 μg/ml, 0.399-10.570 mg/ml and 0.121-3.725 mg/ml, respectively. In summary, pine needles have high polyphenol content, obvious antioxidant activity, strong inhibitory effects on the activities of α-glucosidase, α-amylase and tyrosinase, and have broad application prospects in the development of natural antioxidants and hypoglycemic drugs.

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备注/Memo

备注/Memo:
收稿日期：2022-09-25基金项目：甘肃省中医药重大科研项目(GZKZ-2020-6)；兰州市科技计划项目(2020-ZD-125)作者简介：张家旭(1998-)，男，陕西商洛人，硕士研究生，主要从事中药物质基础及质量控制研究。(E-mail)jiaxu.zhang@outlook.com通讯作者：石晓峰，(E-mail)shixiaofeng2005@sina.com
更新日期/Last Update: 2023-11-17