[1]彭雯杰,詹伊婧,雷鹏,等.阿斯青霉菌XK-12产铁载体特性及其抑菌活性[J].江苏农业学报,2022,38(01):73-80.[doi:doi:10.3969/j.issn.1000-4440.2022.01.008]
　PENG Wen-jie,ZHAN Yi-jing,LEI Peng,et al.Characteristics of siderophores production by Penicilium asturianum XK-12 and its effect on antibacterial activity[J].,2022,38(01):73-80.[doi:doi:10.3969/j.issn.1000-4440.2022.01.008]
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阿斯青霉菌XK-12产铁载体特性及其抑菌活性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年01期
页码:
73-80
栏目:
植物保护
出版日期:
2022-02-28

文章信息/Info

Title:
Characteristics of siderophores production by Penicilium asturianum XK-12 and its effect on antibacterial activity
作者:
彭雯杰; 詹伊婧; 雷鹏; 孙涛; 钱家熠; 徐虹
（南京工业大学食品与轻工学院，江苏南京211816）
Author(s):
PENG Wen-jie; ZHAN Yi-jing; LEI Peng; SUN Tao; QIAN Jia-yi; XU Hong
(College of Food Science and Light Industry, Nanjing Tech University， Nanjing 211816, China)
关键词:
阿斯青霉; 铁载体; 发酵优化; 拮抗作用
Keywords:
Penicilium asturianum; siderophore; fermentation optimization; antagonism
分类号:
S482.2+92
DOI:
doi:10.3969/j.issn.1000-4440.2022.01.008
文献标志码:
A
摘要:
对从健康草莓根际中分离出的一株阿斯青霉菌XK-12（Penicilium asturianum），进行了产铁载体的定性和定量分析，鉴定其所产铁载体为异肟羟酸型铁载体。并对XK-12产铁载体发酵条件进行了优化，利用其发酵液测定了铁载体对病原菌的抑制效果。结果表明，阿斯青霉菌XK-12产铁载体的最佳发酵条件为：3.0% NH4Cl为氮源，2.0%葡萄糖为碳源，添加0.10% L-鸟氨酸盐酸盐，培养基中不含Fe3+，初始pH 6.0，温度28 ℃，250 ml培养瓶装液量为70 ml，发酵周期为168 h；在此条件下，XK-12产铁载体最高能达到0.173 mg/ml。在添加20% (体积分数)XK-12铁载体发酵液条件下，XK-12铁载体发酵液对草莓根腐病病原菌葡萄座腔菌(Neofusicoccum kwambonambiense XKD-1)的抑制率达到了100%。
Abstract:
The qualitative and quantitative analyses of siderophores production were carried out on Penicilium asturianum XK-12 isolated from healthy strawberry rhizosphere. The siderophores produced by XK-12 were identified as hydroxamic acid-type siderophores, and the conditions for producing siderophores were optimized. Finally, the fermentation broth of XK-12 was used to determine the inhibitory effect of siderophores on a pathogen. The results showed that the best fermentation conditions for siderophores production by XK-12 were as follows: 3.0% NH4Cl was used as nitrogen source, 2.0% glucose was used as carbon source, 0.10% of L-ornithine hydrochloride was added, there was no Fe3+ in the medium, the initial pH was 6.0, the temperature was 28 ℃, the liquid volume in 250 ml flask was 70 ml, and the fermentation period was 168 h. Under these conditions, 0.173 mg/ml siderophores could be obtained. Under the condition of adding 20% (volume fraction) XK-12 fermentation broth, the inhibition rate of fermentation broth on strawberry root rot pathogen Neofusicoccum kwambonambiense was 100%.

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备注/Memo

备注/Memo:
收稿日期：2021-04-28基金项目：江苏省重点研发计划（现代农业）项目(BE2019390)作者简介：彭雯杰(1996-)，女，湖北宜昌人，硕士，主要从事农业微生物研究。(E-mail)pwj@njtech.edu.cn通讯作者：徐虹，(E-mail) xuh@njtech.edu.cn
更新日期/Last Update: 2022-03-04