[1]黄桂丽,王毓宁,马佳佳,等.枇杷花多酚对脂肪酶的抑制作用[J].江苏农业学报,2021,(01):192-196.[doi:doi:10.3969/j.issn.1000-4440.2021.01.025]
　HUANG Gui-li,WANG Yu-ning,MA Jia-jia,et al.Inhibitory effect of loquat flowers polyphenolics on lipase activity[J].,2021,(01):192-196.[doi:doi:10.3969/j.issn.1000-4440.2021.01.025]
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枇杷花多酚对脂肪酶的抑制作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
192-196
栏目:
加工贮藏·质量安全
出版日期:
2021-02-28

文章信息/Info

Title:
Inhibitory effect of loquat flowers polyphenolics on lipase activity
作者:
黄桂丽; 王毓宁; 马佳佳; 隋思瑶; 孙灵湘
(苏州市农业科学院，江苏苏州215105)
Author(s):
HUANG Gui-li; WANG Yu-ning; MA Jia-jia; SUI Si-yao; SUN Ling-xiang
(Suzhou Academy of Agricultural Sciences, Suzhou 215105, China)
关键词:
枇杷花多酚; 脂肪酶; 抑制作用
Keywords:
loquat flowers polyphenolics; lipase; inhibitory effect
分类号:
TS201.2
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.025
文献标志码:
A
摘要:
以枇杷花为原料，提取枇杷花多酚，并通过大孔树脂AB-8纯化，得到枇杷花多酚提取物，然后从抑制率、抑制机理、抑制类型和荧光淬灭效应几个方面探究枇杷花多酚对脂肪酶的抑制作用。结果表明，枇杷花多酚对脂肪酶具有很好的抑制效果，半抑制质量浓度为(66.1±6.36) μg/ml，以可逆混合性方式抑制脂肪酶的活性。枇杷花多酚能与脂肪酶生成复合物，结合位点数为1。
Abstract:
The total polyphenolics were extracted and purified by AB-8 macroporous resin from loquat flowers. To investigate the inhibitory kinetics and mechanism of polyphenolics from loquat flowers on lipase, the inhibitory rate, inhibitory mechanism, inhibition type and fluorescence quenhing effect were explored. The results indicated that polyphenolics from loquat flowers showed a remarkable inhibitory effect on lipase activity with half-maximal inhibitory concentration (IC50) value of (66.1±6.36) μg/ml in a mixed-type manner. Loquat flowers polyphenolics can form complex with lipase, and the number binding sites is one.

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备注/Memo

备注/Memo:
收稿日期：2020-04-30基金项目：苏州市科技计划项目(SNG201909); 江苏省农业科技自主创新基金项目[CX(17)3029]作者简介：黄桂丽（1986-），女，河南潢川人，博士，助理研究员，研究方向为食品保鲜与加工。(E-mail)huanggl2015@163.com通讯作者：王毓宁, (E-mail)wyn705@163.com
更新日期/Last Update: 2021-03-15