[1]涂丽琴,吴淑华,高丹娜,等.辽宁省番茄上番茄斑驳花叶病毒的分子鉴定[J].江苏农业学报,2020,(05):1126-1132.[doi:doi:10.3969/j.issn.1000-4440.2020.05.008]
　TU Li-qin,WU Shu-hua,GAO Dan-na,et al.Molecular identification of tomato mottle mosaic virus in tomatoes from Liaoning province[J].,2020,(05):1126-1132.[doi:doi:10.3969/j.issn.1000-4440.2020.05.008]
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辽宁省番茄上番茄斑驳花叶病毒的分子鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年05期
页码:
1126-1132
栏目:
植物保护
出版日期:
2020-10-31

文章信息/Info

Title:
Molecular identification of tomato mottle mosaic virus in tomatoes from Liaoning province
作者:
涂丽琴1; 2; 吴淑华1; 高丹娜1; 2; 吉颖1; 程兆榜1; 周益军1; 朱月林2; 刘勇3; 季英华1
（1.江苏省农业科学院植物保护研究所，江苏南京210014；2.南京农业大学园艺学院，江苏南京210095；3.湖南省农业科学院植物保护研究所，湖南长沙410125）
Author(s):
TU Li-qin1; 2; WU Shu-hua1; GAO Dan-na1; 2; JI Ying1; CHENG Zhao-bang1; ZHOU Yi-jun1; ZHU Yue-lin2; LIU Yong3; JI Ying-hua1
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;3.Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 4110125, China)
关键词:
番茄斑驳花叶病毒; 分子鉴定; 番茄
Keywords:
tomato mottle mosaic virus; molecular identification; tomato
分类号:
S436.412.1+1
DOI:
doi:10.3969/j.issn.1000-4440.2020.05.008
文献标志码:
A
摘要:
为明确2015年在辽宁省调查番茄病毒病时发现的病毒类型，对田间采集的番茄病样进行了分子检测。结果显示，在用烟草花叶病毒属通用检测引物进行RT-PCR扩增时22份样品中有6份扩增到800 bp左右的目的片段，扩增产物测序后BLAST分析结果表明它与已报道的番茄斑驳花叶病毒（Tomato mottle mosaic virus，ToMMV）（KF477193）同源性最高，为99.2%。为进一步确认，针对ToMMV编码的外壳蛋白（CP）设计了一对特异性引物，对样品进行RT-PCR检测，结果显示6份阳性样品都能扩增到目的条带，对其克隆测序后发现CP基因序列全长480 bp，编码1个相对分子质量约1.77×104的蛋白质，系统进化树表明该病毒与ToMMV的同源性最高，共同聚类于一个分支。这些结果表明在辽宁省番茄病株上检测到的病毒为ToMMV的1个分离物，这是在辽宁省番茄上发现番茄斑驳花叶病毒的首次报道。
Abstract:
To clarify the types of viruses found in the process of investigation on tomato virus diseases from Liaoning province in 2015, the infected tomato samples collected in the fields were detected by molecular methods. The results showed that the target fragment of approximately 800 bp long was amplified from six samples of twenty-two samples in the process of RT-PCR amplification, using universal primers for Tobamovirus. The amplified products were sequenced and the results of BLAST analysis showed that, the sequence showed the highest homology with the reported tomato mottle mosaic virus (ToMMV) (KF477193), with a value of 99.2%. To make further confirmation of the above result, a pair of specific primers based on coat protein (CP) sequence coded by ToMMV were designed and used to do RT-PCR detection of the samples. The results showed that the target band could be amplified from six positive samples. The cloning and sequencing results of the target band revealed that the full length of CP gene was 480 bp, encoding a protein with a relative molecular weight of approximately 1.77×104. The results of the phylogenetic tree indicated that the virus sequence showed the highest homology with ToMMV and they all clustered into one branch. The above results indicate that the virus detected in infected tomatoes from Liaoning is an isolate of ToMMV, and this is the first report of ToMMV found in tomatoes from Liaoning province.

参考文献/References:

[1]HANSSEN I M, LAPIDOT M, THOMMA B P. Emerging viral diseases of tomato crops[J]. Molecular Plant-Microbe Interactions, 2010, 23: 539-548.
[2]MOUSSA A B, MAKNI M, MARRAKCHI M. Identification of the principal viruses infecting tomato crops in Tunisia[J]. European and Mediterranean Plant Protection Organization Bulletin, 2010, 30(2): 293-296.
[3]PARRELLA G, CRESCENZI A. The present status of tomato viruses in Italy[J]. Acta Horticlturae, 2005, 695: 37-42.
[4]MASSUMI H, SHAABANIAN M, POUR A H, et al. Incidence of viruses infecting tomato and their natural hosts in the southeast and central regions of Iran[J]. Plant Disease, 2009, 93(1): 67-72.
[5]LI R, GAO S, FEI Z, et al. Complete genome sequence of a new tobamovirus naturally infecting tomatoes in Mexico[J]. Genome Announcements, 2013, 1(5): e00794.
[6]AMBRS S, MARTNEZ F, IVARS P, et al. Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain[J]. European Journal of Plant Pathology, 2017, 149(2): 261-268.
[7]FILLMER K, ADKINS S, PONGAM P, et al. Complete genome sequence of a Tomato mottle mosaic virus isolate from the United States[J]. Genome Announcements, 2015, 3(2): e00167-15.
[8]PADMANABHAN C, ZHENG Y, LI R, et al. Complete genome sequence of a tomato-infecting tomato mottle mosaic virus in New York[J]. Genome Announcements, 2015, 3(6): e01523-15.
[9]TURINA M, GERAATS B P J, CIUFFO M. First report of tomato mottle mosaic virus in tomato crops in Israel[J]. New Disease Reports, 2016, 33: 1.
[10]NAGAI A, DUARTE L, CHAVES A, et al. First Complete genome sequence of an isolate of tomato mottle mosaic virus infecting plants of Solanum lycopersicum in South America.[J]. Genome Announcements, 2018, 6(19): e00427-18.
[11]LI Y Y, WANG C L, XIANG D, et al. First report of tomato mottle mosaic virus infection of pepper in China[J]. Plant Disease, 2014, 98(10): 1447.
[12]LI Y Y, WANG Y, HU J, et al. The complete genome sequence, occurrence and host range of tomato mottle mosaic virus Chinese isolate[J]. Virology Journal, 2017, 14(1): 15.
[13]CHE H Y, LUO D Q, CAO X R, et al. First report of tomato mottle mosaic virus in tomato crops in China[J]. Plant Disease, 2018, 102(10): 2051.
[14]LARTEY R T, VOSS T C, MELCHER U. Tobamovirus evolution: gene overlaps, recombination, and taxonomic implications[J]. Molecular Biology and Evolution, 1996, 13(10): 1327-1338.
[15]吴淑华,赵文浩,李廷芳,等. 南京辣椒上一种斑驳类型病毒病的分子鉴定[J]. 江苏农业学报, 2015, 31(6): 1284-1290.
[16]LETSCHERT B, ADAM G, LESEMANN D E, et al. Detection and differentiation of serologically cross-reacting tobamoviruses of economical importance by RT-PCR and RT-PCR-RFLP[J]. Journal of Virological Methods, 2002, 106(1): 1-10.
[17]ZHAN B H, CAO N, WANG K N, et al. Detection and characterization of an isolate of tomato mottle mosaic virus infecting tomato in China[J]. Journal of Integrative Agriculture, 2018, 17(5): 1207-1212.
[18]LOVELOCK D A, KINOTI W M, BOTTCHER C, et al. Tomato mottle mosaic virus intercepted by Australian biosecurity in Capsicum annuum seed[J]. Australasian Plant Disease Notes, 2020, 15(1): 8.
[19]NAGAI A, DUARTE L M L, CHAVES A L R, et al. Tomato mottle mosaic virus in Brazil and its relationship with Tm-22 gene[J]. European Journal of Plant Pathology, 2019, 155: 353-359.
[20]鲁清华，张宇，张松柏，等. 湖南省长沙市番茄黄化曲叶病毒的检测与系统发育分析[J]. 南方农业学报，2018, 49(7):1332-1337.
[21]郑雪，陈勇，郑立敏，等. 番茄环纹斑点病毒侵染辣椒挥发物成分分析及对西花蓟马行为反应影响[J].山东农业科学，2018, 50(11):111-115.
[22]姜静，王银磊，李亚茹，等. 江苏省及其他地区番茄黄化曲叶病毒的分子鉴定及序列分析[J].江苏农业学报，2018,34(1):238-240.

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备注/Memo

备注/Memo:
收稿日期：2020-03-11基金项目：国家重点研发计划项目（2018YFD0201208）；国家自然科学基金项目（31572074、31770168）；国家特色蔬菜产业技术体系项目（CARS-24-C-01）；江苏省农业自主创新基金项目[CX(18)2005]作者简介：涂丽琴（1996-），女，江西吉安人，博士研究生，研究方向为园艺蔬菜作物病毒。吴淑华为共同第一作者。通讯作者：季英华,(E-mail)jiyinghua@jaas.ac.cn；刘勇,(E-mail)haoasliu@163.com
更新日期/Last Update: 2020-11-16