[1]刘赵月,李蕊彤,李晶,等.盐碱胁迫下京尼平苷对玉米种子萌发及根系AsA-GSH循环的影响[J].江苏农业学报,2020,(04):842-850.[doi:doi:10.3969/j.issn.1000-4440.2020.04.006]
　LIU Zhao-yue,LI Rui-tong,LI Jing,et al.Effects of geniposide on seed germination and AsA-GSH cycle in root of maize under saline-alkali stress[J].,2020,(04):842-850.[doi:doi:10.3969/j.issn.1000-4440.2020.04.006]
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盐碱胁迫下京尼平苷对玉米种子萌发及根系AsA-GSH循环的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年04期
页码:
842-850
栏目:
遗传育种·生理生化
出版日期:
2020-08-31

文章信息/Info

Title:
Effects of geniposide on seed germination and AsA-GSH cycle in root of maize under saline-alkali stress
作者:
刘赵月; 李蕊彤; 李晶; 顾万荣; 左师宇; 任晓松; 左月桃; 魏湜
(东北农业大学农学院，黑龙江哈尔滨150300）
Author(s):
LIU Zhao-yue; LI Rui-tong; LI Jing; GU Wan-rong; ZUO Shi-yu; REN Xiao-song; ZUO Yue-tao;  WEI Shi
(College of Agriculture， Northeast Agricultural University， Harbin 150300， China）
关键词:
盐碱胁迫; 京尼平苷; 玉米; 抗坏血酸-谷胱甘肽循环
Keywords:
saline-alkali stress; geniposide; maize; ascorbate-glutathione cycle
分类号:
S513
DOI:
doi:10.3969/j.issn.1000-4440.2020.04.006
文献标志码:
A
摘要:
为探究京尼平苷(Geniposide，GD）对盐碱胁迫下玉米种子萌发及玉米幼苗形态、生理特性变化的影响，以吉龙2(耐盐碱）和欣煊58(不耐盐碱）为材料，采用营养液水培法培养玉米幼苗，探讨150 mmol/L盐碱胁迫(NaCl∶Na2SO4∶NaHCO3∶Na2CO3=1∶9∶9∶1，摩尔浓度比）条件下，GD对玉米种子萌发、幼苗生长形态、根系抗坏血酸-谷胱甘肽循环(AsA-GSH循环）中抗氧化酶(APX、GR、MDHAR、DHAR）活性及抗氧化物(AsA、GSH）含量的影响。结果表明，在盐碱胁迫下，GD处理能有效缓解盐碱胁迫对玉米种子萌发和玉米幼苗生长的抑制程度；促进吉龙2和欣煊58根系可溶性蛋白质和可溶性糖含量增加，使渗透物质积累保持渗透调节平衡；提高抗氧化酶活性、抗氧化物含量以及AsA/DHA和GSH/GSSG。表明GD能提高植物细胞内的AsA-GSH循环运转效率和玉米幼苗的抗盐碱胁迫能力，本研究为黑龙江盐碱地种植玉米提供了理论依据。
Abstract:
To explore the effects of geniposide (GD) on the seed germination, morphological and physiological characteristics of maize seedlings under saline-alkali stress, Jilong 2 (tolerant to saline-alkali ) and Xinxuan 58 (intolerant to saline-alkali) were used as materials. The effects of GD on seed germination, seedling growth morphology, activities of antioxidant enzymes (APX, GR, MDHAR, DHAR) and contents of antioxidants (AsA, GSH) in the ascorbate-glutathione (AsA-GSH) cycle were investigated under 150 mmol/L saline-alkali stress (molar concentration ratio was NaCl∶Na2SO4∶NaHCO3∶Na2CO3=1∶9∶9∶1) by hydroponic experiment. The results showed that GD significantly promoted seed germination and the growth of maize seedlings under saline-alkali stress, promoted the increase of soluble protein and soluble sugar content in the roots of Jilong 2 and Xinxuan 58, maintained osmotic adjustment balance in osmotic material accumulation, improved antioxidant enzyme activity, the content of antioxidant, AsA/GSH and GSH/GSSG. It was found that GD could improve the efficiency of AsA/GSH cycle in plant cells and the resistance of maize seedlings under saline-alkali stress. The results of this study provide theoretical basis for maize production in saline-alkali land in Heilongjiang province.

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备注/Memo

备注/Memo:
收稿日期：2020-01-06基金项目：国家重点研发计划项目(2016YFD0300103、2017YFD0300506)；国家重点研发计划项目黑龙江省配套资金项目(GX18B029)；东北农业大学“学术骨干”基金项目(17XG23)；黑龙江省博士后科研启动基金项目(LBH-Q16031)；东北农业大学大学生SIPT计划项目作者简介：刘赵月(1995-)，女，黑龙江哈尔滨人，硕士研究生，主要从事玉米逆境生理生态及其调控技术的研究。(Tel）13339415750；(E-mail)liuzhaoyue95@163.com通讯作者：李晶，(E-mail)jingli1027@126.com；顾万荣，(E-mail)wanronggu@163.com
更新日期/Last Update: 2020-09-08