[1]杨恒,时海波,邹烨,等.超声波辅助酸提鸡肺胶原蛋白的持油性和乳化性[J].江苏农业学报,2019,(04):940-947.[doi:doi:10.3969/j.issn.1000-4440.2019.04.027]
 YANG Heng,SHI Hai bo,ZOU Ye,et al.The oilholding capacity and emulsifying properties of collagen from chicken lung extracted by ultrasonicassisted acid method[J].,2019,(04):940-947.[doi:doi:10.3969/j.issn.1000-4440.2019.04.027]
点击复制

超声波辅助酸提鸡肺胶原蛋白的持油性和乳化性()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年04期
页码:
940-947
栏目:
加工贮藏·质量安全
出版日期:
2019-08-31

文章信息/Info

Title:
The oilholding capacity and emulsifying properties of collagen from chicken lung extracted by ultrasonicassisted acid method
作者:
杨恒12时海波2邹烨1王道营1徐为民1李鹏鹏1张新笑1
(1.江苏省农业科学院农产品加工研究所,江苏南京210014;2.南京财经大学食品科学与工程学院,江苏南京210023)
Author(s):
YANG Heng12SHI Haibo2ZOU Ye1WANG Daoying1XU Weimin1LI Pengpeng1ZHANG Xinxiao1
(1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China)
关键词:
鸡肺胶原蛋白超声波辅助提取响应面蛋白质功能特性
Keywords:
collagen of proteins from chicken lungultrasonicassisted extractionresponse surfacefunctional properties
DOI:
doi:10.3969/j.issn.1000-4440.2019.04.027
文献标志码:
A
摘要:
以鸡肺为原料,乙酸为提取剂,采用超声波辅助酸提法提取胶原蛋白,通过BoxBehnken试验设计分析法优化提取工艺。在单因素试验的基础上,以超声波功率、料液比和提取时间为因素,胶原蛋白得率为响应值,设计响应面试验,并比较常规酸提和超声波辅助酸提胶原蛋白的疏水性能、持油性能和乳化性能。结果显示,鸡肺胶原蛋白的超声波辅助酸提最佳条件为超声波功率365 W、料液比1∶90(质量体积比)、提取时间320 min,胶原蛋白得率占除杂后冻干鸡肺(干质量)的39.55%,其得率比常规酸提的提高了371倍;超声波辅助酸提的胶原蛋白疏水性、持油性和乳化活性分别比常规酸提的提高了640%、686%和324%。
Abstract:
Using chicken lung as raw material and acetic acid as extractant, collagen was extracted by ultrasonic assisted acid method, and the extraction technology was optimized by BoxBehnken experimental design analysis. On the basis of the single factor experiment, the ultrasonic power, the ratio of material to liquid and the extraction time were taken as factors. The yield of collagen was the response value, and the response surface test was designed. The hydrophobic properties, oilholding properties and emulsifying properties of collagen by conventional extraction and ultrasonic assisted extraction were compared respectively. The results showed that the optimal extraction conditions of chicken lung collagen were ultrasonic power of 365 W, ratio of material to liquid of 1∶90, extraction time of 320 min, and the yield of collagen accounted for 39.55% of the lungs (dry weight) of freezedried chicken after decontamination. The yield of collagen by ultrasonic assisted extraction was 371 times higher than that by conventional extraction, and the hydrophobicity, oilholding and emulsifying properties of collagen by ultrasonic assisted extraction were 640%, 686% and 324% higher than those by conventional extraction, respectively.

参考文献/References:

[1]WU X, CAI L, CAO A, et al. Comparative study on acidsoluble and pepsinsoluble collagens from skin and swim bladder of grass carp (Ctenopharyngodonidella)[J]. Journal of the Science of Food & Agriculture, 2016, 96(3):815-821.
[2]SUN L, LI B, SONG W, et al. Characterization of pacific cod (Gadusmacrocephalus ) skin collagen and fabrication of collagen sponge as a good biocompatible biomedical material[J]. Process Biochemistry, 2017, 63: 229-235.
[3]PATI F, ADHIKARI B, DHARA S. Isolation and characterization of fish scale collagen of higher thermal stability[J]. Bioresource Technology, 2010, 101(10):3737-3742.
[4]江晃荣.胶原蛋白在医学中的应用[J].健康指南:中老年,2011(1):57.
[5]蔡路昀, 马帅, 曹爱玲,等. 不同部位鲽鱼皮酸溶性胶原蛋白的提取及其特性[J]. 食品科学, 2017, 38(11):52-58.
[6]杨平, 刘影, 公丽艳,等. 酸法和酶法提取草鱼鱼鳞胶原蛋白的特性分析[J]. 食品工业, 2018,39(7):129-132.
[7]CHEN J, LI L, YI R, et al. Release kinetics of Tilapia scale collagen I peptides during tryptic hydrolysis[J]. Food Hydrocolloids, 2018, 77: 931-936.
[8]王沥浩, 王文慧, 郭咏昕, 等. 胶原蛋白功能概述[J]. 黑龙江农业科学, 2014(3): 150-156.
[9]高薇薇, 邓海燕, 谢苗,等. 鸡血的利用现状与展望[J]. 肉类工业, 2004(2):42-46.
[10]晋艳曦. 肉类嫩化新技术—声波嫩化[J]. 肉品卫生, 1999(6):24-25.
[11]邹烨, 蔡盼盼, 王立,等. 超声波辅助酶法提取中华鳖裙边胶原蛋白及其热稳定性能[J]. 食品科学, 2018, 39(2):254-259.
[12]丁建英,王晓飞,张丽,等. 枇杷叶多酚超声波辅助提取工艺优化及其抗氧性分析[J]. 南方农业学报,2018,49(2):340-347.
[13]XIONG G Y, ZHANG L L, ZHANG W, et al. Influence of ultrasound and proteolytic enzyme inhibitors on muscle degradation, tenderness, and cooking loss of hens during aging[J]. Czech Journal of Food Sciences, 2012, 30(3):195-205.
[14]薛芳, 颜瑞, 王承明. 超声波辅助碱提取花生多糖的研究[J]. 食品科学, 2008, 29(8):158-163.
[15]刘俊红,袁冰倩,李爽,等.超声波碱法提取小麦麸皮蛋白质的工艺研究[J].食品工业,2018,39(9):5-8.
[16]王芳,陈晓燕.超声波辅助花生蛋白提取工艺研究[J].化工设计通讯,2017,43(12):133-134.
[17]韩丽丽,杨志伟.响应面优化超声波提取薏米蛋白工艺[J].粮食与油脂,2017,30(12):73-77.
[18]李芳, 绪建荣, 侯伟伟,等. 响应面法优化超声波辅助提取甜杏仁粕蛋白质的研究[J]. 食品工业, 2016(6):149-152.
[19]PIZZOLATO M, BOUTELIER T, DERICHE R. Perfusion deconvolution in DSCMRI with dispersioncompliant bases[J]. Medical Image Analysis, 2017, 36:197-215.
[20]王升力, 黄雪琴, 郭诗,等. 响应面法优化虾壳中虾青素提取工艺的研究[J]. 陕西农业科学, 2016, 62(2):1-5.
[21]BAO Z, CHEN Y Z, WEI X F, et al. Optimization of conditions for collagen extraction from the swim bladders of grass carp (Ctenopharyngodonidella) by response surface methodology[J]. International Journal of Food Engineering, 2010, 6(3):61-64.
[22]JIA J, MA H, ZHAO W, et al. The use of ultrasound for enzymatic preparation of ACEinhibitory peptides from wheat germ protein[J]. Food Chemistry, 2010, 119(1):336-342.
[23]万阅, 齐计英, 曾红,等. 响应面法优化香菇多糖的超声波辅助提取工艺[J]. 生物技术通报, 2015, 31(1):79-85.
[24]王立, 邹烨, 张坤,等. 响应面试验优化超声波辅助碱提鸭肝蛋白工艺及其抗氧化性能[J]. 食品科学, 2017, 38(18):222-228.
[25]曹荣锟,李佳泰,王金晶,等.大米辅料啤酒中蛋白质疏水性与蛋白质泡沫稳定性的分析[J].食品与发酵工业,2018,44(9):66-70.
[26]GU X, LYDIA J C. Influence of sugars on the characteristics of gluconodlactoneinduced soy protein isolate gels[J]. Food Hydrocolloids, 2009, 23(2):314-326.
[27]杨勇, 毕爽, 王中江,等. 超声波处理对绿豆蛋白结构及功能特性的影响[J]. 食品工业科技, 2016, 37(9):69-73.
[28]刘昕, 金明良, 覃小丽,等. 超声波处理对鹰嘴豆蛋白乳化性的影响[J]. 食品与发酵工业, 2018,44(5):142-147.
[29]孙英杰. 超声波处理对大豆分离蛋白结构和功能性质影响研究[D]. 哈尔滨:东北农业大学, 2014.
[30]谢为峰, 罗丰收, 解超男,等. 超声波处理对杏仁粕蛋白质理化和功能性质的改性研究[J]. 农产品加工, 2016(17):1-3.
[31]温青玉, 杨帆, 盛威,等. 小麦分离蛋白质理化性质及功能特性研究[J]. 河南农业科学, 2018,47(5):149-154.
[32]王笑宇, 韩东, 陈子净, 等. 超声波处理对β伴大豆球蛋白乳化性能的影响[J]. 中国食品学报, 2018,18(6):153-159.
[33]LEALCALDERON F, THIVILLIERS F, SCHMITT V. Structured emulsions[J]. Current Opinion in Colloid & Interface Science, 2007, 12(4):206-212.

相似文献/References:

[1]武中庸,热孜万古力·赛买提,车敏娜,等.响应面法优化紫色马铃薯花青素超声波提取工艺[J].江苏农业学报,2017,(06):1379.[doi:doi:10.3969/j.issn.1000-4440.2017.06.026]
 WU Zhong-yong,RE ZIWANGULI Sai-mai-ti,CHE Min-na,et al.Optimization of ultrasonic-assisted extraction process for anthocyanin from purple potato by response surface analysis[J].,2017,(04):1379.[doi:doi:10.3969/j.issn.1000-4440.2017.06.026]

备注/Memo

备注/Memo:
收稿日期:2018-12-22 基金项目:国家现代农业(肉鸡)产业技术体系建设专项(CARS-41);江苏省农业科技自主创新基金项目[CX(18)1006];江苏省自然科学基金项目(BK20180300);江苏省农业科学院基本科研业务专项资金项目[ZX(18)3009] 作者简介:杨恒(1994-),男,浙江湖州人,硕士研究生,研究方向为肉品加工与质量控制。(E-mail)heng199491@126.com 通讯作者:邹烨,(E-mail)zouye@jaas.ac.cn
更新日期/Last Update: 2019-08-31