[1]张焕新,郑义,殷玲,等.白背三七黄酮类化合物的提取及抗氧化性评价[J].江苏农业学报,2019,(04):933-939.[doi:doi:10.3969/j.issn.1000-4440.2019.04.026]
　ZHANG Huan xin,ZHENG Yi,YIN Ling,et al.Extraction and ontioxidant evaluation of flavonoids from Gynura divaricata (L.) DC. Leaves[J].,2019,(04):933-939.[doi:doi:10.3969/j.issn.1000-4440.2019.04.026]
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白背三七黄酮类化合物的提取及抗氧化性评价()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年04期
页码:
933-939
栏目:
加工贮藏·质量安全
出版日期:
2019-08-31

文章信息/Info

Title:
Extraction and ontioxidant evaluation of flavonoids from Gynura divaricata (L.) DC. Leaves
作者:
张焕新; 郑义; 殷玲; 董志俭
(江苏农牧科技职业学院，江苏泰州225300)
Author(s):
ZHANG Huanxin; ZHENG Yi; YIN Ling; DONG Zhijian
(Jiangsu Agrianimal Husbandry Vocational College, Taizhou 225300, China)
关键词:
白背三七; 黄酮; 抗氧化
Keywords:
Gynura divaricate (L.) DC.; flavonoids; antioxidant activity
分类号:
S567.23+6
DOI:
doi:10.3969/j.issn.1000-4440.2019.04.026
文献标志码:
A
摘要:
采用乙醇提取法从白背三七叶中提取具有生物活性的黄酮类化合物，并对其抗氧化活性进行综合评价。通过正交试验，确定了白背三七黄酮类化合物提取的最优工艺条件：乙醇体积分数80%，料液比1∶25(质量体积比)，提取温度65 ℃，提取时间120 min。在此条件下，黄酮类化合物平均得率3.81%；抗氧化试验结果表明白背三七黄酮类化合物具有较强的抗氧化能力，且呈明显的剂量效应。对Fe3+的还原能力与VC接近（P＞005），而远高于BHT；对DPPH自由基和ABTS自由基均具有很好的清除效果，其IC50值分别为0039 mg/ml和0062 mg/ml。白背三七黄酮类化合物对DPPH自由基的清除能力极显著高于对照组BHT 和VC（P＜001），对ABTS自由基清除能力显著强于VC，但不如BHT。
Abstract:
Flavonoids with biological activity were extracted from Gynura divaricate (L.) DC. leaves by ethanol method, and their antioxidant activities were evaluated comprehensively. The extraction technique was optimized using orthogonal experiments, and the optimum extraction parameters were as follows: ethanol concentration 80%, materialliquid ratio 1∶25, extracting temperature 65 ℃, extracting time 120 min, and under these conditions, the yield of flavonoids was 381%. The results of antioxidant test showed that the flavonoids had strong antioxidant capacity and obvious dose effect ability of Fe3+ was close to that of VC (P＞005), but much higher than that of BHT. The ability of scavenging DPPH and ABTS free radical was strong, and their IC50 values were 0039 mg/ml and 0062 mg/ml, respectively. The scavenging ability of DPPH radical was significantly higher than that of BHT and VC (P＜001), and the scavenging ability of ABTS radical was significantly stronger than that of VC, but not as good as that of BHT.

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备注/Memo

备注/Memo:
收稿日期：2018-10-16 基金项目：国家自然科学青年基金项目（31502020）；江苏省“青蓝工程”项目（20160418）；泰州市“311”人才培养工程项目；江苏农牧科技职业学院院级课题(NSF201505-2) 作者简介：张焕新（1972-），男，河南叶县人，博士，教授，主要从事粮食、油脂及植物蛋白工程研究。hxinzh@hotmail.com 通讯作者：郑义，（E-mail）Zysm76@163.com
更新日期/Last Update: 2019-08-31