[1]杨海峰,惠林冲,潘美红,等.洋葱黄色条纹突变体的特征特性[J].江苏农业学报,2019,(01):157-165.[doi:doi:10.3969/j.issn.1000-4440.2019.01.023]
　YANG Hai-feng,HUI Lin-chong,PAN Mei-hong,et al.The characteristics of yellow stripe mutant in onion (Allium cepa L.)[J].,2019,(01):157-165.[doi:doi:10.3969/j.issn.1000-4440.2019.01.023]
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洋葱黄色条纹突变体的特征特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年01期
页码:
157-165
栏目:
园艺
出版日期:
2019-02-26

文章信息/Info

Title:
The characteristics of yellow stripe mutant in onion (Allium cepa L.)
作者:
杨海峰; 惠林冲; 潘美红; 陈微; 何林玉; 王江英; 缪美华; 陈振泰
（连云港市农业科学院，江苏连云港222000）
Author(s):
YANG Hai-feng; HUI Lin-chong; PAN Mei-hong; CHEN Wei; HE Lin-yu; WANG Jiang-ying; MIAO Mei-hua; CHEN Zhen-tai
（Lianyungang Academy of Agricultural Sciences， Lianyungang 222000, China）
关键词:
洋葱; 黄色条纹; 突变体
Keywords:
onion (Allium cepa L.); yellow stripe; mutant
分类号:
S633.2
DOI:
doi:10.3969/j.issn.1000-4440.2019.01.023
文献标志码:
A
摘要:
对母系遗传洋葱叶片黄色条纹突变体材料进行田间表型特征观察，叶绿素含量测定和显微结构观察，并利用jnurf13分子标记鉴定保持株。结果表明，洋葱突变体为亮绿黄色条纹。黄皮、白皮和紫皮洋葱中均发现黄色条纹突变株，黄化程度越高植株长势越弱；整个生育期黄色条纹一直存在于管状叶、花薹、花苞、伞状花序中花梗、花瓣、雌蕊、雄蕊等部位，直至植株枯萎也不返绿。单株自交或开放式自然授粉F1中出现全黄株、黄绿条纹株和绿株3种类型，无固定分离比，全黄株苗期死亡。洋葱黄色条纹突变体黄色组织的总叶绿素、叶绿素a和叶绿素b含量显著低于绿色组织；石蜡切片观察结果显示黄色和绿色组织的形态结构无差异，但绿色组织的叶绿体总数高于黄色组织；通过超显微结构观察发现，黄色条纹突变体绿色组织中叶绿体结构与正常株无差异，而黄色组织中类囊体、基粒等结构降解，嗜饿颗粒数量多且集中，影响光合作用。另外，利用jnurf13分子标记从12株可育突变体中筛选出5株保持株，可作为形态学标记用于洋葱细胞质雄性杂交制种。
Abstract:
In this study, field phenotypic characteristics of maternally inherited onion leaf stripe mutants, chlorophyll content and microstructure were observed. In addition, jnurf13 molecular markers were used to identify the maintained strains. The results showed that the onion mutants were bright green-yellow stripes and were found in yellow, white and purple onions. The higher the degree of yellowing, the weaker the growth of the plants. Yellow stripes have been accompanied by tubular leaves, calyx, peduncles, petals, pistils, stamens, until withered and not green. Single-plant self-cross or open natural pollination F1 appeared in all three types of whole yellow, yellow stripes and green strains, no fixed separation ratio and the whole yellow plant died at seedling stage. The contents of total chlorophyll, chlorophyll a and chlorophyll b in yellow tissue of onion yellow stripe mutant were significantly lower than those in green tissue. The results of paraffin sections observation showed that there was no difference in morphological structure between green and yellow tissues, but the total number of chloroplasts in green tissues was higher than that in yellow tissues. Ultrastructural observation revealed that there was no difference in chloroplast structure between the green mutant tissue and the normal strain, whereas thylakoid and grana in yellow tissue were degraded, and the number of hungry particles was numerous and concentrated, affecting photosynthesis. In addition, five conserved strains were screened out from 12 fertile mutants using the jnurf13 molecular marker and used as a morphological marker for onion cytoplasmic male hybrid production.

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备注/Memo

备注/Memo:
收稿日期：2018-05-16 基金项目：江苏省农业科技自主创新基金项目[CX(17)2020]；连云港市财政专项（QNJJ1706）；连云港市“521”项目[连人才办(2017)4号] 作者简介：杨海峰（1978-），男，贵州黄平人，本科，副研究员，从事洋葱育种、栽培及分子生物学研究。(E-mail)13775492959@163.com 通讯作者：陈振泰，(E-mail)13812349618@163.com
更新日期/Last Update: 2019-02-27