[1]王凤婷,靳盼盼,刘芳,等.乳酸对粪肠球菌的抑菌作用及作用机制[J].江苏农业学报,2018,(01):200-206.[doi:doi:10.3969/j.issn.1000-4440.2018.01.029]
　WANG Feng-ting,JIN Pan-pan,LIU Fang,et al.Antimicrobial activity and mechanism of lactic acid on Enterococcus faecalis[J].,2018,(01):200-206.[doi:doi:10.3969/j.issn.1000-4440.2018.01.029]
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乳酸对粪肠球菌的抑菌作用及作用机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
200-206
栏目:
加工贮藏·质量安全
出版日期:
2018-02-25

文章信息/Info

Title:
Antimicrobial activity and mechanism of lactic acid on Enterococcus faecalis
作者:
王凤婷1; 2; 靳盼盼1; 2; 刘芳1; 孙芝兰1; 吴海虹1; 王道营1; 许晓曦2; 徐为民1
（1.江苏省农业科学院农产品加工研究所，江苏南京210014；2.东北农业大学食品学院，黑龙江哈尔滨150030）
Author(s):
WANG Feng-ting1; 2; JIN Pan-pan1; 2; LIU Fang1; SUN Zhi-lan1; WU Hai-hong1; WANG Dao-ying1; XU Xiao-xi2; XU Wei-min1
(1.Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Food Science, Northeast Agricultural University, Harbin 150030, China)
关键词:
粪肠球菌; 乳酸; 抑菌机理
Keywords:
Enterococcus faecalis; lactic acid; antibacterial mechanism
分类号:
TS201.3
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.029
文献标志码:
A
摘要:
目前乳酸常被用来控制食品中腐败菌的生长，但是有关其对食品优势腐败菌抑菌机制方面的研究较少。本试验以一株从肉制品中分离得到的腐败菌粪肠球菌R612-Z1为目标菌株，研究乳酸对其的杀菌效果及作用机制。结果表明，各浓度（0.25%、0.50%、1.00%）的乳酸均有杀菌效果而且作用明显，通过激光共聚焦显微镜及流式细胞仪检测发现，处理后细胞膜通透性增强。扫描电镜及腺嘌呤核苷三磷酸（ATP）浓度的测定结果说明，胞内物质流出聚集在细胞外侧，胞外ATP浓度和核酸类物质浓度明显上升，膜电势快速升高。因此，乳酸通过破坏粪肠球菌的细胞壁，增加细胞膜通透性，改变细胞内外电势，导致内容物流出，从而达到杀菌效果。
Abstract:
Lactic acid (LA) is widely used to control the growth of contaminating bacteria in food, but there are few reports about the antibacterial mechanism of it. The antimicrobial activity of LA against a dominated spoilage bacterium, Enterococcus faecalis R612-Z1, isolated from water-boiled salted duck was studied. The results showed that all the selected concentrations of LA (0.25%, 0.50%, 1.00%) had obvious bactericidal action. The permeability of cell membranes was increased for the bacterial cells treated with 1.00% LA， which was detected using the laser scanning confocal microscope and flow cytometry. The intracellular substances were leaked according to the results of scanning electron micrographs and the detection of extracellular ATP and UV-absorbing materials. The membrane potentials were found to be increased. Therefore, the antibacterial effect of LA on E. faecalis was mainly completed by the massive leakage of intracellular component, which was caused by damaging the cell membrane and membrane potential.

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备注/Memo

备注/Memo:
收稿日期：2017-06-22 基金项目：国家自然科学基金项目（31371802）；江苏省自然科学基金项目（BK20151367）；江苏省农业科技自主创新基金项目[CX(17)3015];江苏省重点研发计划项目（BE2017392） 作者简介：王凤婷（1993-），女，黑龙江哈尔滨人，硕士研究生，主要从事食品微生物研究。（E-mail）526013836@qq.com 通讯作者：刘芳，（E-mail）fangliu82@163.com
更新日期/Last Update: 2018-03-06