[1]王帆,张添菊,王兴娜,等.麝香草酚对单增李斯特菌的抑制作用[J].江苏农业学报,2017,(06):1402-1407.[doi:doi:10.3969/j.issn.1000-4440.2017.06.029]
 WANG Fan,ZHANG Tian-ju,WANG Xing-na,et al.Inhibitory effect of thymol against Listeria monocytogenes[J].,2017,(06):1402-1407.[doi:doi:10.3969/j.issn.1000-4440.2017.06.029]
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麝香草酚对单增李斯特菌的抑制作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1402-1407
栏目:
加工贮藏·质量安全
出版日期:
2017-12-30

文章信息/Info

Title:
Inhibitory effect of thymol against Listeria monocytogenes
作者:
王帆1张添菊12王兴娜1周涛2李春阳1
(1.江苏省农业科学院农产品加工研究所,江苏南京210014;2.南京师范大学金陵女子学院,江苏南京210097)
Author(s):
WANG Fan1ZHANG Tian-ju12WANG Xing-na1ZHOU Tao2LI Chun-yang1
(1.Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Jinling College, Nanjing Normal University, Nanjing 210097, China)
关键词:
麝香草酚单增李斯特菌作用机制
Keywords:
thymolListeria monocytogenesmechanism
分类号:
Q935
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.029
文献标志码:
A
摘要:
为开发以麝香草酚为主要成分的新型抑菌剂。采用微量肉汤稀释法测定麝香草酚对单增李斯特菌的最小抑制浓度和最小致死浓度分别为156.25 μg/ml和312.50 μg/ml。通过扫描电镜观察发现,经过麝香草酚处理后单增李斯特菌细胞出现穿孔式膜损伤。进一步研究结果表明,麝香草酚分别处理1 h、2 h和3 h后,单增李斯特菌胞外的碱性磷酸酶、葡萄糖和核酸含量显著高于对照。SDS-PAGE电泳分析结果显示,麝香草酚处理6 h后单增李斯特菌胞内蛋白含量降低,说明麝香草酚处理使得单增李斯特菌细胞通透性增加,导致细胞内的物质流失到细胞外。碘化丙啶(PI)染色结合倒置荧光显微镜观察结果显示,麝香草酚处理造成了单增李斯特菌细胞膜的破损,并最终导致菌体细胞死亡。
Abstract:
The inhibitory effect of thymol against Listeria monocytogenes was investigated in this study. The minimum inhibitory concentration and minimal bactericidal concentration of thymol were 156.25 μg/ml and 312.50 μg/ml respectively determined by micro broth dilution method. Scanning electron microscopic observation revealed that the surface structure of thymol-treated L. monocytogenes was damaged. Extracellular contents of alkaline phosphatase, glucose and DNA from L. monocytogenes were significantly higher than those of control after treated with thymol for 1-3 h. The SDS-PAGE pattern of intracellular soluble proteins of L. monocytogenes was shallow after thymol treatment for 6 h, suggesting that thymol caused the increase of membrane permeability and the leakage of intracellular substances from L. monocytogenes. Observation under inverted fluorescence microscope after PI stain indicated that thymol induced the membrane damage and cell death of L. monocytogenes.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-04-10 基金项目:国家自然科学基金项目(NSF31401615) 作者简介:王帆(1983-),女,江苏南京人,硕士,助理研究员,研究方向为食品微生物。(E-mail) wangfan713@126.com 通讯作者:李春阳,(E-mail)lichunyang968@126.com;王兴娜,(E-mail)xingnawang.nn@163.com
更新日期/Last Update: 2018-01-03